Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats
Aim: To investigate the influence of exogenous lactoferrin (LF) on tumor growth, energy and lipid metabolism of Walker-256 carcinosarcoma and to assess genotoxic effects of LF. Materials and Methods: The study was performed on Walker-256 tumor-bearing rats. Total lipids and phospholipids were determ...
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| Published in: | Experimental Oncology |
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| Date: | 2018 |
| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2018
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/145710 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats / V.F. Chekhun, D.M. Storchai, I.N. Todor, T.V. Borikun, N.Yu. Lukianova // Experimental Oncology. — 2018 — Т. 40, № 3. — С. 200–204. — Бібліогр.: 28 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862637294800863232 |
|---|---|
| author | Chekhun, V.F. Storchai, D.M. Todor, I.N. Borikun, T.V. Lukianova, N.Yu. |
| author_facet | Chekhun, V.F. Storchai, D.M. Todor, I.N. Borikun, T.V. Lukianova, N.Yu. |
| citation_txt | Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats / V.F. Chekhun, D.M. Storchai, I.N. Todor, T.V. Borikun, N.Yu. Lukianova // Experimental Oncology. — 2018 — Т. 40, № 3. — С. 200–204. — Бібліогр.: 28 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: To investigate the influence of exogenous lactoferrin (LF) on tumor growth, energy and lipid metabolism of Walker-256 carcinosarcoma and to assess genotoxic effects of LF. Materials and Methods: The study was performed on Walker-256 tumor-bearing rats. Total lipids and phospholipids were determined by thin-layer chromatography. Comet assay was used to investigate the genotoxic effects of LF. Results: Daily i.p. administrations of exogenous LF at concentrations of 1 mg/kg and 10 mg/kg starting from the 4th day after tumor transplantation suppressed growth of Walker-256 carcinosarcoma by almost 44%. After treatment with recombinant LF in both doses, the phospholipid composition of Walker-256 carcinosarcoma cells was changed (3-fold increase of phosphatidylethanolamine, 3.4-fold increase of phosphatidylcholine, and 1.8-fold increase of sphingomyelin, while the cardiolipin content decreased by 67%. Exogenous LF was not genotoxic for bone marrow cells (as assessed by the ratio of PCE/NCE, number of micronuclei) and peripheral blood lymphocytes (percentage of DNA in the tail of a comet) in Walker-256 carcinosarcoma-bearing rats. Conclusion: Exogenous LF caused the inhibition of Walker-256 carcinosarcoma growth and a decrease in the microviscosity of plasma cell membranes, and exerted no genotoxicity toward bone marrow cells and peripheral blood of experimental animals. Key Words: lactoferrin, breast cancer, Walker-256 carcinosarcoma, phospholipid content, genotoxicity.
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| first_indexed | 2025-11-30T22:44:02Z |
| format | Article |
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| id | nasplib_isofts_kiev_ua-123456789-145710 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-11-30T22:44:02Z |
| publishDate | 2018 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Chekhun, V.F. Storchai, D.M. Todor, I.N. Borikun, T.V. Lukianova, N.Yu. 2019-01-26T21:05:52Z 2019-01-26T21:05:52Z 2018 Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats / V.F. Chekhun, D.M. Storchai, I.N. Todor, T.V. Borikun, N.Yu. Lukianova // Experimental Oncology. — 2018 — Т. 40, № 3. — С. 200–204. — Бібліогр.: 28 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/145710 Aim: To investigate the influence of exogenous lactoferrin (LF) on tumor growth, energy and lipid metabolism of Walker-256 carcinosarcoma and to assess genotoxic effects of LF. Materials and Methods: The study was performed on Walker-256 tumor-bearing rats. Total lipids and phospholipids were determined by thin-layer chromatography. Comet assay was used to investigate the genotoxic effects of LF. Results: Daily i.p. administrations of exogenous LF at concentrations of 1 mg/kg and 10 mg/kg starting from the 4th day after tumor transplantation suppressed growth of Walker-256 carcinosarcoma by almost 44%. After treatment with recombinant LF in both doses, the phospholipid composition of Walker-256 carcinosarcoma cells was changed (3-fold increase of phosphatidylethanolamine, 3.4-fold increase of phosphatidylcholine, and 1.8-fold increase of sphingomyelin, while the cardiolipin content decreased by 67%. Exogenous LF was not genotoxic for bone marrow cells (as assessed by the ratio of PCE/NCE, number of micronuclei) and peripheral blood lymphocytes (percentage of DNA in the tail of a comet) in Walker-256 carcinosarcoma-bearing rats. Conclusion: Exogenous LF caused the inhibition of Walker-256 carcinosarcoma growth and a decrease in the microviscosity of plasma cell membranes, and exerted no genotoxicity toward bone marrow cells and peripheral blood of experimental animals. Key Words: lactoferrin, breast cancer, Walker-256 carcinosarcoma, phospholipid content, genotoxicity. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats Article published earlier |
| spellingShingle | Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats Chekhun, V.F. Storchai, D.M. Todor, I.N. Borikun, T.V. Lukianova, N.Yu. Original contributions |
| title | Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats |
| title_full | Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats |
| title_fullStr | Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats |
| title_full_unstemmed | Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats |
| title_short | Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats |
| title_sort | antitumor and genotoxic effects of lactoferrin in walker-256 tumor-bearing rats |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/145710 |
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