Caffeine Suppresses GABA Receptor-Mediated Current in Rat Primary Sensory Neurons Via Inhibition of Intracellular Phosphodiesterase

• Date: 2015
• Main Authors: Yang, J.Y., Yang, G., Ren, J., Zhao, J., Li Sh.
• Format: Article
• Language: English
• Published: Інститут фізіології ім. О.О. Богомольця НАН України 2015
• Series: Нейрофизиология
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/148186
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Caffeine Suppresses GABA Receptor-Mediated Current in Rat Primary Sensory Neurons Via Inhibition of Intracellular Phosphodiesterase / J.Y. Yang, G. Yang, J. Ren, J. Zhao, Sh. Li // Нейрофизиология. — 2015. — Т. 47, № 2. — С. 129-135. — Бібліогр.: 25 назв. — англ.

In acutely isolated rat primary sensory neurons, the effects of caffeine on GABA receptormediated current (IGABA) were investigated using a whole-cell patch clamp technique. We found that applications of GABA (10-1000 µM) induced inward currents in a concentration-dependent manner; the currents manifested obvious desensitization. Pretreatment with caffeine (0.01- 100 µM) suppressed IGABA in a noncompetitive manner; caffeine shifted the concentration – response curve for GABA downwards compared to the control. Theophylline showed a similar and stronger inhibitory effect on IGABA. Isolated application of 1 µM diazepam enhanced IGABA, while pretreatment with 10 µM caffeine and 1 µM diazepam suppressed this current. Intracellular application of the protein kinase A inhibitor H-8 dramatically weakened the inhibitory effect of caffeine on IGABA. Because primary afferent depolarization is related to GABAA receptors, our results suggest that caffeine might antagonize presynaptic inhibitory effects of primary afferents, probably via inhibition of intracellular phosphodiesterase.