Stress-Induced Increases in the Levels of Caspases in the Prefrontal Cortex in a Rat Model of PTSD

We investigated the levels of expression of caspase-3 and 9 in the medial prefrontal cortex (mPFC) of rats subjected to single prolonged stress (SPS), trying to provide a novel insight into the mechanism of how this cortical region is related to post-traumatic stress disorder (PTSD). Fifty male W...

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Bibliographic Details
Date:2016
Main Authors: Zhang, J.H., Li, M., Han, F., Yu X. Shi
Format: Article
Language:English
Published: Інститут фізіології ім. О.О. Богомольця НАН України 2016
Series:Нейрофизиология
Online Access:https://nasplib.isofts.kiev.ua/handle/123456789/148322
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Journal Title:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Cite this:Stress-Induced Increases in the Levels of Caspases in the Prefrontal Cortex in a Rat Model of PTSD / J.H. Zhang, M. Li, F. Han, Yu.X. Shi // Нейрофизиология. — 2016. — Т. 48, № 1. — С. 14-19. — Бібліогр.: 23 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
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Summary:We investigated the levels of expression of caspase-3 and 9 in the medial prefrontal cortex (mPFC) of rats subjected to single prolonged stress (SPS), trying to provide a novel insight into the mechanism of how this cortical region is related to post-traumatic stress disorder (PTSD). Fifty male Wistar rats were divided into the control group and four SPS groups examined at days 1,4,7, and 14 after treatment. Expression of caspase-3 in SPS groups was significantly greater when compared with the control group (P < 0.05) and peaked at day 7 after exposure to SPS. In the control group, the intensity of fluorescence of caspase-9- positive cells was low, while that in the SPS groups was significantly higher (P < 0.01) and peaked at day 4 after exposure to SPS. After SPS episodes, levels of mRNA of caspase-3 and caspase-9, compared with those in the control group, gradually increased and peaked at days 7 and 4, respectively (P < 0.01). Therefore, changes of expression of caspase-9 and caspase-3 may play an important role in the pathogenesis of PTSD.