Recombinant Staphylococcal protein A with cysteine residue for preparation of affinity chromatography stationary phase and immunosensor applications

• Published in: Вiopolymers and Cell
• Date: 2015
• Main Authors: Gorbatiuk, O.B., Bakhmachuk, A.O., Dubey, L.V., Usenko, M.O., Irodov, D.M., Okunev, O.V., Kostenko, O.M., Rachkov, A.E., Kordium, V.A.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2015
• Subjects: Molecular and Cell Biotechnologies
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/152442
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Recombinant Staphylococcal protein A with cysteine residue for preparation of affinity chromatography stationary phase and immunosensor applications / O.B. Gorbatiuk, A.O. Bakhmachuk, L.V. Dubey, M.O. Usenko, D.M. Irodov, O.V. Okunev, O.M. Kostenko, A.E. Rachkov, V.A. Kordium // Вiopolymers and Cell. — 2015. — Т. 31, № 2. — С. 115-122. — Бібліогр.: 22 назв. — англ.

Aim. Engineering of recombinant Staphylococcal protein A with cysteine residue (SPA-Cys) for preparation of affinity chromatography stationary phase and formation of bioselective element of immunosensor. Methods. DNA sequences encoding IgG-binding region of SPA, His-tag and cysteine were genetically...