Construction, expression, functional characterization and practical application of fusion protein SPA-BAPmut
Aim. The creation of genetically engineered fusion protein SPA-BAPmut and its application as a secondary immunoreagent in immunoassays. Methods. Gene cloning, PCR, electrophoresis, DNA sequencing, bacteria cells culturing, protein expression and purification, ELISA, Western-blotting were used. Resul...
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| Veröffentlicht in: | Вiopolymers and Cell |
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| Datum: | 2013 |
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | English |
| Veröffentlicht: |
Інститут молекулярної біології і генетики НАН України
2013
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| Schlagworte: | |
| Online Zugang: | https://nasplib.isofts.kiev.ua/handle/123456789/152487 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Construction, expression, functional characterization and practical application of fusion protein SPA-BAPmut / O.B. Gorbatiuk, O.V. Okunev, Yu.S. Nikolaev, O.V. Svyatenko, V.A. Kordium // Вiopolymers and Cell. — 2013. — Т. 29, №. 1. — С. 49-54. — Бібліогр.: 12 назв. — англ, укр. |