Construction, expression, functional characterization and practical application of fusion protein SPA-BAPmut

• Published in: Вiopolymers and Cell
• Date: 2013
• Main Authors: Gorbatiuk, O.B., Okunev, O.V., Nikolaev, Yu.S., Svyatenko, O.V., Kordium, V.A.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2013
• Subjects: Molecular and Cell Biotechnologies
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/152487
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Construction, expression, functional characterization and practical application of fusion protein SPA-BAPmut / O.B. Gorbatiuk, O.V. Okunev, Yu.S. Nikolaev, O.V. Svyatenko, V.A. Kordium // Вiopolymers and Cell. — 2013. — Т. 29, №. 1. — С. 49-54. — Бібліогр.: 12 назв. — англ, укр.

Aim. The creation of genetically engineered fusion protein SPA-BAPmut and its application as a secondary immunoreagent in immunoassays. Methods. Gene cloning, PCR, electrophoresis, DNA sequencing, bacteria cells culturing, protein expression and purification, ELISA, Western-blotting were used. Resul...