Identification of Ca²⁺/calmodulin-dependent phosphorylation sites of endocytic scaffold ITSN1 by tandem mass spectrometry

• Published in: Вiopolymers and Cell
• Date: 2015
• Main Authors: Morderer, D.Ye., Nikolaienko, O.V., Rynditch, A.V.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2015
• Subjects: Structure and Function of Biopolymers
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/152592
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Identification of Ca²⁺/calmodulin-dependent phosphorylation sites of endocytic scaffold ITSN1 by tandem mass spectrometry / D.Ye. Morderer, O.V. Nikolaienko, A.V. Rynditch // Вiopolymers and Cell. — 2015. — Т. 31, № 5. — С. 338-344. — Бібліогр.: 28 назв. — англ.

ITSN1 is a scaffold protein involved in endocytosis, signal transduction and cytoskeleton regulation. It has been previously shown that ITSN1 undergoes Ca²⁺/calmodulin-dependent phosphorylation in vitro. Aim. We intend to identify these phosphorylation sites. Methods. In vitro kinase reaction; liqui...