Efficiency of application of different DNA probes in identifying marker chromosomes

• Date: 2016
• Main Authors: Tavokina, L.V., Brovko, A.O., Baronova, E.V., Moskalenko, E.P., Gorovenko, N.G.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2016
• Series: Вiopolymers and Cell
• Subjects: Biomedicine
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/152767
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Efficiency of application of different DNA probes in identifying marker chromosomes / L.V. Tavokina, A.O. Brovko, E.V. Baronova, E.P. Moskalenko, N.G. Gorovenko // Вiopolymers and Cell. — 2016. — Т. 32, № 1. — С. 49-53. — Бібліогр.: 9 назв. — англ.

The presence of marker chromosomes in the human karyotype always requires a special diagnostic approach. Determination of the marker chromosome type and structure is of great diagnostic and prognostic importance. There are several methods of marker chromosomes identification, which differ in their informative value. The paper presents the results of cytogenetic and FISH diagnostics of supernumerical marker chromosomes (SMC) cases in patients’ karyotype. Aim. To analyze the results of the cytogenetic and molecular cytogenetic diagnostics for patients with marker chromosomes, and to evaluate and compare the efficiency of the methods used. Methods. Karyotyping was done according to the standard methods. GTG, CBG, QFQ and NOR-Ag methods of differential staining were used. FISH was performed according to the manufacturer’s instructions for CEP, LSI and WCP DNA-probes. Results. Marker chromosome was found in 15 of 7989 patients. Application of standard staining methods was effective in 66.6 % of cases. Combination of differential staining and FISH allowed identifying a marker chromosome in 83.3 %. 90 % of all marker chromosomes were identified as isochromosomes and 60 % of them were derivative from chromosome 15. Conclusions. The use of WCP probes is a main step in the marker chromosome identification with further application of CEP/LSI probes. If a marker chromosome has nonspecific DNA sequences more sensitive methods should be use.