Intranuclear localization of transcription factories and immunoglobulin heavy chain gene alleles during human B-cell maturation

• Published in: Вiopolymers and Cell
• Date: 2016
• Main Authors: Pichugin, A.V., Iarovaia, O.V., Sklyar, I.V., Lacombe, G., Razin, S.V., Fest, T., Lipinski, M., Vassetzky, Y.S.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2016
• Subjects: Structure and Function of Biopolymers
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/152827
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Intranuclear localization of transcription factories and immunoglobulin heavy chain gene alleles during human B-cell maturation / A.V. Pichugin, O.V. Iarovaia, I.V. Sklyar, G. Lacombe, S.V. Razin, T. Fest, M. Lipinski, Y.S. Vassetzky // Вiopolymers and Cell. — 2016. — Т. 32, № 3. — С. 179-183. — Бібліогр.: 18 назв. — англ.

Immunoglobulin heavy chain (IGH) gene locus is expressed monoallelically in human B cells. Aim. To study the role of nuclear organization in regulation of the IGH expression during B-cell differentiation. Methods. Immunofluorescence in situ hybridization on 3D-preserved nuclei (3D immuno-FISH). Resu...