Practical approach to quantification of mRNA abundance using RT-qPCR, normalization of experimental data and MIQE

• Published in: Вiopolymers and Cell
• Date: 2016
• Main Authors: Obolenskaya, M.Yu., Kuklin, A.V., Rodrigez, R.R., Martsenyuk, O.P., Korneyeva, K.L., Docenko, V.A., Draguschenko, O.O.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2016
• Subjects: Reviews
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/152828
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Practical approach to quantification of mRNA abundance using RT-qPCR, normalization of experimental data and MIQE / M.Yu. Obolenskaya, A.V. Kuklin, R.R. Rodrigez, O.P. Martsenyuk, K.L. Korneyeva, V.A. Docenko, O.O. Draguschenko // Вiopolymers and Cell. — 2016. — Т. 32, № 3. — С. 161-172. — Бібліогр.: 46 назв. — англ.

Reverse transcription and quantitative polymerase chain reaction (RT-qPCR) has become the most common method for characterizing expression patterns of individual mRNAs due to a large dynamic range of linear quantification, high speed, sensitivity, resolution and cost-effectiveness. However, the comp...