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Practical approach to quantification of mRNA abundance using RT-qPCR, normalization of experimental data and MIQE

Reverse transcription and quantitative polymerase chain reaction (RT-qPCR) has become the most common method for characterizing expression patterns of individual mRNAs due to a large dynamic range of linear quantification, high speed, sensitivity, resolution and cost-effectiveness. However, the comp...

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Veröffentlicht in:	Вiopolymers and Cell
Datum:	2016
Hauptverfasser:	Obolenskaya, M.Yu., Kuklin, A.V., Rodrigez, R.R., Martsenyuk, O.P., Korneyeva, K.L., Docenko, V.A., Draguschenko, O.O.
Format:	Artikel
Sprache:	Englisch
Veröffentlicht:	Інститут молекулярної біології і генетики НАН України 2016
Schlagworte:	Reviews
Online Zugang:	https://nasplib.isofts.kiev.ua/handle/123456789/152828
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!
Назва журналу:	Digital Library of Periodicals of National Academy of Sciences of Ukraine
Zitieren:	Practical approach to quantification of mRNA abundance using RT-qPCR, normalization of experimental data and MIQE / M.Yu. Obolenskaya, A.V. Kuklin, R.R. Rodrigez, O.P. Martsenyuk, K.L. Korneyeva, V.A. Docenko, O.O. Draguschenko // Вiopolymers and Cell. — 2016. — Т. 32, № 3. — С. 161-172. — Бібліогр.: 46 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine

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