Conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination
The characteristics of conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination have been studied. Urease has been immobilized by cross-linking with bovine serum albumin using glutaraldehyde. The resulting conductivity changes are produced by enzymatic...
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| Published in: | Биополимеры и клетка |
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| Date: | 1996 |
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| Format: | Article |
| Language: | English |
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Інститут молекулярної біології і генетики НАН України
1996
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/153663 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination / S.V. Dzyadevich, G.A. Zhylyak, A.P. Soldatkin // Биополимеры и клетка. — 1996. — Т. 12, № 1. — С. 53-57. — Бібліогр.: 9 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| id |
nasplib_isofts_kiev_ua-123456789-153663 |
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Dzyadevich, S.V. Zhylyak, G.A. Soldatkin, A.P. 2019-06-14T12:12:07Z 2019-06-14T12:12:07Z 1996 Conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination / S.V. Dzyadevich, G.A. Zhylyak, A.P. Soldatkin // Биополимеры и клетка. — 1996. — Т. 12, № 1. — С. 53-57. — Бібліогр.: 9 назв. — англ. 0233-7657 https://nasplib.isofts.kiev.ua/handle/123456789/153663 577.15+573.6 The characteristics of conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination have been studied. Urease has been immobilized by cross-linking with bovine serum albumin using glutaraldehyde. The resulting conductivity changes are produced by enzymatically catalyzed hydrolysis of urea. This process for both immobilized enzyme and soluble one is described by the classic laws of enzymatic kinetics. The influence of ionic strength, pH and buffer capacity of the samples on the biosensor response has been thoroughly tested. The results have been discussed concerning urea concentration analysis in human blood. A part of this work was supported by a grant from State Committee of Ukraine on Science and Technology (grant No. 5.3/21). en Інститут молекулярної біології і генетики НАН України Биополимеры и клетка Conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination Кондуктометричний уреазний мікробіосенсор для визначення сечовини на основі тонкошіівкових гребінчастих електродів Кондуктометрический уреазный микробиосенсор для определения мочевины на основе тонкопленочных гребенчатых электродов Article published earlier |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| title |
Conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination |
| spellingShingle |
Conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination Dzyadevich, S.V. Zhylyak, G.A. Soldatkin, A.P. |
| title_short |
Conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination |
| title_full |
Conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination |
| title_fullStr |
Conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination |
| title_full_unstemmed |
Conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination |
| title_sort |
conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination |
| author |
Dzyadevich, S.V. Zhylyak, G.A. Soldatkin, A.P. |
| author_facet |
Dzyadevich, S.V. Zhylyak, G.A. Soldatkin, A.P. |
| publishDate |
1996 |
| language |
English |
| container_title |
Биополимеры и клетка |
| publisher |
Інститут молекулярної біології і генетики НАН України |
| format |
Article |
| title_alt |
Кондуктометричний уреазний мікробіосенсор для визначення сечовини на основі тонкошіівкових гребінчастих електродів Кондуктометрический уреазный микробиосенсор для определения мочевины на основе тонкопленочных гребенчатых электродов |
| description |
The characteristics of conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination have been studied. Urease has been immobilized by cross-linking with bovine serum albumin using glutaraldehyde. The resulting conductivity changes are produced by enzymatically catalyzed hydrolysis of urea. This process for both immobilized enzyme and soluble one is described by the classic laws of enzymatic kinetics. The influence of ionic strength, pH and buffer capacity of the samples on the biosensor response has been thoroughly tested. The results have been discussed concerning urea concentration analysis in human blood.
|
| issn |
0233-7657 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/153663 |
| citation_txt |
Conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination / S.V. Dzyadevich, G.A. Zhylyak, A.P. Soldatkin // Биополимеры и клетка. — 1996. — Т. 12, № 1. — С. 53-57. — Бібліогр.: 9 назв. — англ. |
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2025-12-07T16:31:35Z |
| last_indexed |
2025-12-07T16:31:35Z |
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