Interaction of different tRNAs with translation elongation factors 1A from lower and higher eukaryotes

• Published in: Вiopolymers and Cell
• Date: 2009
• Main Authors: Futernyk, P.V., Negrutskii, B.S., El'skaya, A.V.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2009
• Subjects: Структура та функції біополімерів
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/153843
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Interaction of different tRNAs with translation elongation factors 1A from lower and higher eukaryotes / P.V. Futernyk. B.S. Negrutskii, A.V. El'skaya // Вiopolymers and Cell. — 2009. — Т. 25, № 6. — С. 457-465. — Бібліогр.: 43 назв. — англ.

The work is aimed at confirmation of earlier assumed mechanism of tRNA channeling. Methods. The methods of band shift assay and Forsters resonance energy transfer were used. Results. The affinities of mammalian tRNAs for two tissue specific isoforms of elongator factor eEF1A1 and eEF1A2 were compare...