Implementation of the quantitative Real-Time PCR for the molecular-genetic diagnostics of spinal muscular atrophy

• Veröffentlicht in: Вiopolymers and Cell
• Datum: 2010
• Hauptverfasser: Soloviov, O.O., Livshits, G.B., Podlesnaya, S.S., Livshits, L.A.
• Format: Artikel
• Sprache: Englisch
• Veröffentlicht: Інститут молекулярної біології і генетики НАН України 2010
• Schlagworte: Genomics, Transcriptomics and Proteomics
• Online Zugang: https://nasplib.isofts.kiev.ua/handle/123456789/153859
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Zitieren: Implementation of the quantitative Real-Time PCR for the molecular-genetic diagnostics of spinal muscular atrophy / O.O. Soloviov, G.B. Livshits, S.S. Podlesnaya, L.A. Livshits, // Вiopolymers and Cell. — 2010. — Т. 26, № 1. — С. 51-55. — Бібліогр.: 14 назв. — англ.

Aim. To develop an easy and reliable assay for quantitative analysis of the SMN1 gene exon 7 copy number with Real-Time PCR and a SYBR Green dye which can be used as a test-system for spinal muscular atrophy (SMA) diagnostics. Methods. For the quantification the SMN1 gene exon 7 copies we have used...