Comparative analysis of human and mammalias genes of protein ortologs of cytochrome P450 2E1
Aim. To carry out a comparison analysis of nucleotide sequences for the cytochrome P450 2E1 protein ortologs genes and reestablish the connection of gene evolution with nucleotide content. Methods. In silico: BLAST, ClustalW, MEGA4, PHP. Results. A general phylogeny of CYP2E1 genes is described. The...
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| Опубліковано в: : | Вiopolymers and Cell |
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| Дата: | 2010 |
| Автори: | , , , |
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| Мова: | Англійська |
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Інститут молекулярної біології і генетики НАН України
2010
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Comparative analysis of human and mammalias genes of protein ortologs of cytochrome P450 2E1 / D.R. Duplij, O.V. Maksymchuk, V.P. Duplij, N.A. Chaschin // Вiopolymers and Cell. — 2010. — Т. 26, № 4. — С. 322-326. — Бібліогр.: 13 назв. — англ, рос. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1860224909903396864 |
|---|---|
| author | Duplij, D.R. Maksymchuk, O.V. Duplij, V.P. Chaschin, N.A. |
| author_facet | Duplij, D.R. Maksymchuk, O.V. Duplij, V.P. Chaschin, N.A. |
| citation_txt | Comparative analysis of human and mammalias genes of protein ortologs of cytochrome P450 2E1 / D.R. Duplij, O.V. Maksymchuk, V.P. Duplij, N.A. Chaschin // Вiopolymers and Cell. — 2010. — Т. 26, № 4. — С. 322-326. — Бібліогр.: 13 назв. — англ, рос. |
| collection | DSpace DC |
| container_title | Вiopolymers and Cell |
| description | Aim. To carry out a comparison analysis of nucleotide sequences for the cytochrome P450 2E1 protein ortologs genes and reestablish the connection of gene evolution with nucleotide content. Methods. In silico: BLAST, ClustalW, MEGA4, PHP. Results. A general phylogeny of CYP2E1 genes is described. The most affinity is found for human translated sequences with Pan troglodytes. The transition C > T is the most often, it occurs in introns by 2.6 times more than in exons. The correlation of keto-amino skew of CYP2E1 genes and evolution age of species is stated. Conclusions. The analysis carried out in the paper allows us to assume that a common ancestor of the CYP2E1 protein isoform lived before the divergence between rodent and Primates orders, i.e. 70 million years ago. The single nucleotide substitution is accumulated in introns during evolution.
Цель. Провести сравнительный анализ нуклеотидных последовательностей генов ортологичных белков цитохрома Р450 2Е1 и установить связь эволюции гена с нуклеотидным составом. Методы. In silico: BLAST, ClustalW, MEGA4, PHP. Результаты. Изучены особенности общей филогении генов CYP2E1. Наибольшее родство транслируемой последовательности гена CYP2E1 Homo sapiеns обнаружено с Pan troglodytes. Выявлено, что транзиция С Т встречается в интронах в 2,6 раза чаще, чем в экзонах. Установлена связь между кето-аминовой асимметрией генов CYP2E1 и эволюционным возрастом вида. Выводы. На примере CYP2E1 показано, что в течение эволюции интронный состав генов изменяется в сторону увеличения количества гуанина и тимина. Таким образом, величину кето-аминовой асимметрии можно использовать как дополнительный критерий эволюционного анализа.
Мета. Провести порівняльний аналіз нуклеотидних послідовностей генів ортологічних білків цитохрому Р450 2Е1 і встановити зв’язок еволюції гена з нуклеотидним складом. Методи. In silico: BLAST, ClustalW, MEGA4, PHP. Результати. Описано особливості загальної філогенії генів CYP2E1. Найбільшу спорідненість трансльованої послідовності гена CYP2E1 Homo sapiеns виявлено з Pan troglodytes. Визначено, що транзиція С Т зустрічається в інтронах у 2,6 разу частіше, ніж в экзонах. Встановлено зв’язок між кето-аміновою асиметрією генів CYP2E1 і еволюційним віком виду. Висновки. На прикладі CYP2E1 показано, що впродовж еволюції нуклеотидний склад інтронів змінюється у напрямку збільшення кількості гуаніну і тиміну. Таким чином, величину кето-аміновой асиметрії можна використовувати як додатковий критерій еволюційного аналізу.
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| first_indexed | 2025-12-07T18:19:30Z |
| format | Article |
| fulltext |
Comparative analysis of human and mammalias genes of
protein ortologs of cytochrome P450 2E1
D. R. Duplij, Î. V. Maksymchuk, V. P. Duplij1, N. À. Chaschin
Institute of Molecular Biology and Genetics
150 Academician Zabolotny Street, Kiev, Ukraine, 03143
1Institute of Cell Biology and Genetic Engineering
148 Academician Zabolotny Street, Kiev, Ukraine, 03680
duplijv@icbge.org.ua; duplijd@gmail.com
Aim. To carry out a comparison analysis of nucleotide sequences for the cytochrome P450 2E1 protein
ortologs genes and re- establish the connection of gene evolution with nucleotide con- tent. Methods. In
silico: BLAST, ClustalW, MEGA4, PHP. Results. A general phylogeny of CYP2E1 genes is described. The
most affinity is found for human translated sequences with Pan troglo- dytes. The transition Ñ > T is the
most often, it occurs in introns by 2.6 times more than in exons. The correlation of keto-amino skew of
CYP2E1 genes and evolution age of species is stated. Conclusions. The analysis carried out in the paper
allows us to assume that a common ancestor of the CYP2E1 protein isoform lived before the divergence
between rodent and Primates orders, i.e. 70 million years ago. The single nucleotide substitution is
accumulated in int- rons during evolution.
Keywords: cytochrome Ð450 2Å1, CYP2E1, transition, phylogeny, nucleotide composition skew.
Introduction. The multigen family of cytochrome
P450 is one of the most intensively studied issues,
because it plays an important role in metabolism of
endo- and exogenous substratum. The ethanol
inducible isoform of P450 2E1 cytochrome (CYP2E1)
is of a special interest [1].
The induction of Cyp2E1 expression leads to an
increase in the level of oxygen radicals generated by
CYP2E1, which are able to initiate the
NADPH-depended lipid peroxidation. Because of this
the cell acid-base balance is hereupon violated, and the
oxidative stress develops [2]. The interaction of the
genetic and environmental factors results in the
tissue-specific changes of synthesis and activity of
322
ISSN 1993-6842. Biopolymers and cell. 2010. vol. 26. N 4. Translated from Ukrainian
Ó Institute of Molecular Biology and Genetics NAS of Ukraine, 2010
Cyp2E1, which brings in a certain contribution to the
pathogeny of liver, pancreas diseases and other. [3, 4, 5].
There are some data about the impact of exogenous
factors on expression of Cyp2E1 [6], while the
influence of genetic factors on expression of enzyme
has not been studied enough. From the evolutional
viewpoint, the cytochrome P450 is a unique protein [7],
which survived from the primitive aerobes-prokaryotes
to the human. This allows us to believe that most
modern genes of cytochromes had a general
predecessor, existed about 2 billions of years ago [8].
An opinion exists, that the primary evolutional
function of cytochromes P450 in a cell was connected
with its participation in the plastic and power
metabolisms. The cytochrome P450 got the main
specialization as xenobiotic biotransformator about
800 million years ago, which coincides with time of
animals appearance [8].
In this paper, a comparative analysis of nucleotide
sequences of mammalian and human genes of the
Cyp2E1 orthology proteins is made for the restoration
of evolutional history of the gene and mutational
events.
Materials and methods The genomic sequences
CYP2E1 of human and seven mammals (Pan
troglodytes XM_508139.2, Bos taurus
NM_174530.2, Canis lupus NM_001003339.1,
Equus caballus NM_001111303.1, Mus musculus
NM_021282.2, Rattus norvegicus NM_031543.1, Sus
scrofa NM_214421 [9] ) were used in the
investigation.
The ini tial align ment of nu cle o tide se quences was
re al ized by BLASTN [10] and mul ti ple align ment was
ex e cuted by ClustalW [11]. The phylo gen etic anal y sis
was car ried out by the MEGA4 packet [12]. The anal -
y sis of sin gle nu cle o tide sub sti tu tions was ful filled by
us ing PHP com puter lan guage [13]. The value of
keto-amino skew (Kskew) was cal cu lated us ing the for -
mula Kskew= (NG+NT -NA -NC)/L, where NA, NG,
NT, NC are ab so lute quan ti ties of cor re spond ing nu -
cleo tides in a frag ment of length (L = NA + NG + NT
+ NC) [14]. The ar ith met i cal mean val ues of Kskew for
exons and introns se quences were cal cu lated, and the
stan dard er ror of sam ple was found us ing the for mula
STD_ERR=RMSD/vN, where N is the size of a sam -
ple [15].
Results and discussions The structure of the
Cyp2E1 genes coincides for the majority of studied
mammals. It consists of nine exons and eight introns. A
unique variant of mRNA Cyp2E1 is described for all
species, in which the translated sequence of gene
begins in the first exon and terminates in the last one. In
spite of identical number of exons, the length of
Cyp2E1 differs for different species due to the varied
introns length.
Thus, Homo sapiens has the longest sixth intron
(due to the repetitive GGG sequence of 576 b.p.), and
Mus musculus has the longest second intron. The
structural likeness of the intron parts of CYP2E from
Rattus norvegicus and Mus musculus is of special
interest. They have around 86% similarity between the
sequences of second and sixth introns, and 93% –
between the fourth introns.
To reconstruct the evolutional history of the P450
2E1 cytochrome gene the multiple alignments of the
translated sequences were executed and a phylogenetic
tree was built (Fig.1).
It follows from Fig.1, that the evolution of CYP2E1
gene undergoes a few divergences; in particular, there
are independent ways of rodents, primates and other
mammalian genes development. It is possible that the
analyzed CYP2E1 genes had a common ancestor
before the division of rodents and primates that is about
70 millions years ago.
The pairwise alignments of the CYP2E1 exonic and
intronic sequences between each species and human
were executed and frequencies of single nucleotide
323
COMPARATIVE ANALYSIS OF GENES OF PROTEIN ORTOLOGS OF CYTOCHROME P450 2E1
Rattus norvegicus
Bos taurus
Sus scrofa
Canis lupus
Equus cabbalus
Homo sapiens
Pan troglodytes
Mus musculus
0,00 óñë. åä.0,020,040,060,080,100,12
Fig. 1. Phylogenetic tree of the P450 2E1 cytochrome genes for
human and seven mammals. Numerals mark evolutional distances
in the reference units.
substitutions were analyzed in homologous sites to
establish the nucleotide composition changes of the
examined genes during their evolution.
Twelve possible types of single nucleotide
substitutions were estimated. It should be noted that the
substantial distinctions in frequencies of individual
types of replacements in exonic and inronic parts of
genes were found out. In particular, the A - G
transitions were observed more frequent, than the T- C
ones, and the G-A transitions were observed more
frequent than the C-T ones. The final results are
presented in Table. The substitutions are found to be
more frequent in introns, than in exons. At the same
time the transitions in exons are by 23% more frequent,
than transversions.
Rather small predominance (about 5%). of
transversions was observed in introns due to the
transition G>C, which, possibly, related to the species
features of CpG rich areas, where Cyp2E1 is located.
This fact is of special interest and requires a more
thorough research.
The fixed distinctions between nucleotide ratios in
different functional portions of genes were shown in
our previous work [14]. Indeed, the high values of the
keto-amine skew of nucleotide composition (Kskew)
were registered in introns. This reflects predominance
of the most frequent types of substituted nucleotides
during the spontaneous mutagenesis. In order to check
changes of Kskew value in evolution, we calculated the
value Kskew for introns and exons of Cyp2E1 in each
species and compared it with early obtained data [14].
The changes in the Kskew value depending on the
evolutional affinity of nucleotide sequences is
presented in Fig.2.
Fig.2A shows that the introns Kskew values at
evolution remote from Homo sapiens are negative due
to the predominance of A and C. During evolution the
Kskew value increases from -0,041±0,021 (Mus
DUPLIJ D. R. ET AL.
324
Name of species
compared
with Homo sapiens
Frequencies of single nucleotide substitutions
Transi tions Trans versions Total Insertions Deletions
Exons
Pan troglodytes 0,014 0,011 0,025 0,067 0,003
Sus scrofa 0,111 0,092 0,203 0,035 0,000
Bos taurus 0,107 0,087 0,194 0,108 0,000
Canis upus 0,119 0,098 0,217 0,045 0,004
Equus cabbalus 0,110 0,081 0,192 0,045 0,035
Mus musculus 0,132 0,095 0,227 0,054 0,001
Rattus norvegicus 0,128 0,088 0,217 0,000 0,017
Introns
Pan troglodytes 0,025 0,024 0,049 0,081 0,001
Sus scrofa 0,211 0,235 0,446 0,078 0,029
Bos taurus 0,200 0,196 0,396 0,017 0,143
Canis lupus 0,198 0,213 0,411 0,010 0,185
Equus cabbalus 0,208 0,223 0,431 0,037 0,048
Mus musculus 0,236 0,265 0,501 0,015 0,074
Rattus norvegicus 0,198 0,187 0,386 0,013 0,142
Total frequencies of mutational events in gene CYP2E1
musculus ) and -0,042±0,032 (Bos taurus ) to
0,004±0,016 (Pan troglodytes), and for Homo sapiens
it arrives 0,017±0,027. On the contrary, all the exons
Kskew values are negative (Fig.2B) and similar for eight
mammalian species. This fact can be explained by the
accumulation of thymine and guanine in the loci of the
least selection pressure.
Thus, the Kskew value can be of particular interest for
further research, because it can serve as an additional
criterion for the estimation of nucleotide sequence
evolutional age. The presented numerical analysis of
the gene sequences of P450 2E1 cythochrome
orthologous proteins allowed us to describe general
regularities of the Cyp2E1 phylogeny. We have shown
that the frequencies of single nucleotide substitutions
in the Cyp2E1 introns are by 2.62 times higher in
comparison with the exons .The most frequent
mutational events are transitions which come to 48% of
all single nucleotide substitutions.
Ä. Ð. Äóï ëèé, Î. Â. Ìàê ñèì ÷óê, Â. Ï. Äóï ëèé, Í. À. ×à ùèí
Ñðàâ íè òåëü íûé àíà ëèç ãå íîâ îðòî ëî ãè÷ íûõ áåë êîâ öè òîõ ðî ìà
Ð450 2Å1 ÷å ëî âå êà è ìëå êî ïè òà þ ùèõ
Ðå çþ ìå
Öåëü. Ïðî âåñ òè ñðàâ íè òåëü íûé àíà ëèç íóê ëå î òèä íûõ ïî ñëå äî -
âà òåëü íîñ òåé ãå íîâ îðòî ëî ãè÷ íûõ áåë êîâ öè òîõ ðî ìà Ð450
2Å1 è óñòà íî âèòü ñâÿçü ýâî ëþ öèè ãåíà ñ íóê ëå î òèä íûì ñî ñòà -
âîì. Ìå òî äû. In silico: BLAST, ClustalW, MEGA4, PHP. Ðå çóëü -
òà òû. Èçó ÷å íû îñî áåí íîñ òè îá ùåé ôè ëî ãå íèè ãå íîâ CYP2E1.
Íà è áîëü øåå ðî äñòâî òðàíñ ëè ðó å ìîé ïî ñëå äî âà òåëü íîñ òè
ãåíà CYP2E1 Homo sapiåns îá íà ðó æå íî ñ Pan troglodytes. Âû ÿâ -
ëå íî, ÷òî òðàí çè öèÿ Ñ ®Ò âñòðå ÷à åò ñÿ â èí òðî íàõ â 2,6 ðàçà
÷àùå, ÷åì â ýê çî íàõ. Óñòà íîâ ëå íà ñâÿçü ìåæ äó êåòî-àìè íî âîé
àñèì ìåò ðè åé ãå íîâ CYP2E1 è ýâî ëþ öè îí íûì âîç ðàñ òîì âèäà.
Âû âî äû. Íà ïðè ìå ðå CYP2E1 ïî êà çà íî, ÷òî â òå ÷å íèå ýâî ëþ -
öèè èí òðîí íûé ñî ñòàâ ãå íîâ èç ìå íÿ åò ñÿ â ñòî ðî íó óâå ëè ÷å íèÿ
êî ëè ÷åñ òâà ãó à íè íà è òè ìè íà. Òà êèì îá ðà çîì, âå ëè ÷è íó
êåòî-àìè íî âîé àñèì ìåò ðèè ìîæ íî èñ ïîëü çî âàòü êàê äî ïîë -
íè òåëü íûé êðè òå ðèé ýâî ëþ öè îí íî ãî àíà ëè çà.
Êëþ ÷å âûå ñëî âà: öè òîõ ðîì Ð450 2Å1, CYP2E1, òðàí çè öèè,
ôè ëî ãå íèÿ, àñèì ìåò ðèè íóê ëå î òèä íî ãî ñî ñòà âà.
COMPARATIVE ANALYSIS OF GENES OF PROTEIN ORTOLOGS OF CYTOCHROME P450 2E1
325
–0,11
–0,09
–0,07
–0,05
–0,03
–0,01
0,01
0,03
0,05
1 2 3 4 5 6 7 8
à
1 2 3 4 5 6 7 8
á
Fig. 2. Values of Kskew in introns (a) and exons (b) of the CYP2E1 genes for eight mammals. The biological species are located on abscises in
order of their affinity to the CYP2E1 gene: 1 – Mus musculus; 2 – Rattus norvegicus; 3 – Pan troglodytes; 4 – Homo sapiens; 5 – Equus
caballus; 6 – Canis lupus; 7 – Sus scrofa; 8 – Bos taurus. The proper averages of Kskew are indicated on ordinate for introns (A) and exons (B).
The dotted line marks the average Kskew value for 10839 human genes [14].
–0,11
–0,09
–0,07
–0,05
–0,03
–0,01
0,01
0,03
0,05
70 70 45 30 30 23 13 5
Fig. 3. Values Ks CYP2E1 introns of eight mammalian. The
divergention time (MY) of species is denoted at abscissa axis [16]:
70-Mus musculus, Rattus norvegicus; 45- Equus caballus; 30 -Bos
taurus; Canis lupus; 23- Sus scrofa; 13-Pan troglodytes; 5-Homo
sapiens. The corresponding mean Ks values with standard errors are
depicted at ordinate axis.
Ä. Ð. Äóïë³é, Î. Â. Ìàê ñèì ÷óê, Â. Ï. Äóïë³é, M. O. ×à ùèí
Ïîð³âíÿëü íèé àíàë³ç ãåí³â îðòî ëîã³÷íèõ á³ëê³â öè òîõ ðî ìó Ð450
2Å1 ëþ äè íè ³ ññàâö³â
Ðå çþ ìå
Ìåòà. Ïðî âåñ òè ïîð³âíÿëü íèé àíàë³ç íóê ëå î òèä íèõ ïîñë³äîâ -
íîñ òåé ãåí³â îðòî ëîã³÷íèõ á³ëê³â öè òîõ ðî ìó Ð450 2Å1 ³ âñòà -
íî âè òè çâ’ÿ çîê åâî ëþö³¿ ãåíà ç íóê ëå î òèä íèì ñêëà äîì.
Ìå òî äè. In silico: BLAST, ClustalW, MEGA4, PHP. Ðå çóëü òà -
òè. Îïè ñàíî îñîá ëè âîñò³ çà ãàëü íî¿ ô³ëî ãåí³¿ ãåí³â CYP2E1.
Íàéá³ëüøó ñïîð³äíåí³ñòü òðàíñ ëüî âà íî¿ ïîñë³äîâ íîñò³ ãåíà
CYP2E1 Homo sapiåns âè ÿâ ëåíî ç Pan troglodytes. Âèç íà ÷å íî, ùî
òðàí çèö³ÿ Ñ ®Ò çóñòð³÷àºòüñÿ â ³íòðî íàõ ó 2,6 ðàçó ÷àñò³øå,
í³æ â ýê çî íàõ. Âñòà íîâ ëå íî çâ’ÿ çîê ì³æ êåòî-àì³íî âîþ àñè -
ìåòð³ºþ ãåí³â CYP2E1 ³ åâî ëþö³éíèì â³êîì âèäó. Âèñ íîâ êè. Íà
ïðè êëàä³ CYP2E1 ïî êà çà íî, ùî âïðî äîâæ åâî ëþö³¿ íóê ëå î òèä -
íèé ñêëàä ³íòðîí³â çì³íþºòüñÿ ó íàïðÿìêó çá³ëüøåí íÿ ê³ëü-
êîñò³ ãóàí³íó ³ òèì³íó. Òà êèì ÷è íîì, âå ëè ÷è íó êåòî-àì³íî âîé
àñè ìåò𳿠ìîæ íà âè êî ðèñ òî âó âà òè ÿê äî äàò êî âèé êðè òåð³é
åâî ëþö³éíî ãî àíàë³çó.
Êëþ ÷îâ³ ñëî âà: öè òîõ ðîì Ð450 2Å1, CYP2E1, òðàí çèö³¿,
ô³ëî ãåí³ÿ, àñè ìåò𳿠íóê ëå î òèä íî ãî ñêëà äó.
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DUPLIJ D. R. ET AL.
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| id | nasplib_isofts_kiev_ua-123456789-154131 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 0233-7657 |
| language | English |
| last_indexed | 2025-12-07T18:19:30Z |
| publishDate | 2010 |
| publisher | Інститут молекулярної біології і генетики НАН України |
| record_format | dspace |
| spelling | Duplij, D.R. Maksymchuk, O.V. Duplij, V.P. Chaschin, N.A. 2019-06-15T08:45:52Z 2019-06-15T08:45:52Z 2010 Comparative analysis of human and mammalias genes of protein ortologs of cytochrome P450 2E1 / D.R. Duplij, O.V. Maksymchuk, V.P. Duplij, N.A. Chaschin // Вiopolymers and Cell. — 2010. — Т. 26, № 4. — С. 322-326. — Бібліогр.: 13 назв. — англ, рос. 0233-7657 DOI: http://dx.doi.org/10.7124/bc.000167 https://nasplib.isofts.kiev.ua/handle/123456789/154131 577.3 Aim. To carry out a comparison analysis of nucleotide sequences for the cytochrome P450 2E1 protein ortologs genes and reestablish the connection of gene evolution with nucleotide content. Methods. In silico: BLAST, ClustalW, MEGA4, PHP. Results. A general phylogeny of CYP2E1 genes is described. The most affinity is found for human translated sequences with Pan troglodytes. The transition C > T is the most often, it occurs in introns by 2.6 times more than in exons. The correlation of keto-amino skew of CYP2E1 genes and evolution age of species is stated. Conclusions. The analysis carried out in the paper allows us to assume that a common ancestor of the CYP2E1 protein isoform lived before the divergence between rodent and Primates orders, i.e. 70 million years ago. The single nucleotide substitution is accumulated in introns during evolution. Цель. Провести сравнительный анализ нуклеотидных последовательностей генов ортологичных белков цитохрома Р450 2Е1 и установить связь эволюции гена с нуклеотидным составом. Методы. In silico: BLAST, ClustalW, MEGA4, PHP. Результаты. Изучены особенности общей филогении генов CYP2E1. Наибольшее родство транслируемой последовательности гена CYP2E1 Homo sapiеns обнаружено с Pan troglodytes. Выявлено, что транзиция С Т встречается в интронах в 2,6 раза чаще, чем в экзонах. Установлена связь между кето-аминовой асимметрией генов CYP2E1 и эволюционным возрастом вида. Выводы. На примере CYP2E1 показано, что в течение эволюции интронный состав генов изменяется в сторону увеличения количества гуанина и тимина. Таким образом, величину кето-аминовой асимметрии можно использовать как дополнительный критерий эволюционного анализа. Мета. Провести порівняльний аналіз нуклеотидних послідовностей генів ортологічних білків цитохрому Р450 2Е1 і встановити зв’язок еволюції гена з нуклеотидним складом. Методи. In silico: BLAST, ClustalW, MEGA4, PHP. Результати. Описано особливості загальної філогенії генів CYP2E1. Найбільшу спорідненість трансльованої послідовності гена CYP2E1 Homo sapiеns виявлено з Pan troglodytes. Визначено, що транзиція С Т зустрічається в інтронах у 2,6 разу частіше, ніж в экзонах. Встановлено зв’язок між кето-аміновою асиметрією генів CYP2E1 і еволюційним віком виду. Висновки. На прикладі CYP2E1 показано, що впродовж еволюції нуклеотидний склад інтронів змінюється у напрямку збільшення кількості гуаніну і тиміну. Таким чином, величину кето-аміновой асиметрії можна використовувати як додатковий критерій еволюційного аналізу. en Інститут молекулярної біології і генетики НАН України Вiopolymers and Cell Short Communications Comparative analysis of human and mammalias genes of protein ortologs of cytochrome P450 2E1 Порівняльний аналіз генів ортологічних білків цитохрому Р450 2Е1 людини і ссавців Сравнительный анализ генов ортологичных белков цитохрома Р450 2Е1 человека и млекопитающих Article published earlier |
| spellingShingle | Comparative analysis of human and mammalias genes of protein ortologs of cytochrome P450 2E1 Duplij, D.R. Maksymchuk, O.V. Duplij, V.P. Chaschin, N.A. Short Communications |
| title | Comparative analysis of human and mammalias genes of protein ortologs of cytochrome P450 2E1 |
| title_alt | Порівняльний аналіз генів ортологічних білків цитохрому Р450 2Е1 людини і ссавців Сравнительный анализ генов ортологичных белков цитохрома Р450 2Е1 человека и млекопитающих |
| title_full | Comparative analysis of human and mammalias genes of protein ortologs of cytochrome P450 2E1 |
| title_fullStr | Comparative analysis of human and mammalias genes of protein ortologs of cytochrome P450 2E1 |
| title_full_unstemmed | Comparative analysis of human and mammalias genes of protein ortologs of cytochrome P450 2E1 |
| title_short | Comparative analysis of human and mammalias genes of protein ortologs of cytochrome P450 2E1 |
| title_sort | comparative analysis of human and mammalias genes of protein ortologs of cytochrome p450 2e1 |
| topic | Short Communications |
| topic_facet | Short Communications |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/154131 |
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