Interaction of cyanine dyes with nucleic acids. 4. Efficient 5-fluorescent labelling of oligonucleotides with monomethyne pyrylium cyanine dye, Cyan 39

• Veröffentlicht in: Биополимеры и клетка
• Datum: 1998
• Hauptverfasser: Yarmoluk, S.M., Kostenko, A.M., Kornushyna, O.S., Dubey, I.Y.
• Format: Artikel
• Sprache: Englisch
• Veröffentlicht: Інститут молекулярної біології і генетики НАН України 1998
• Schlagworte: Методы
• Online Zugang: https://nasplib.isofts.kiev.ua/handle/123456789/154137
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Zitieren: Interaction of cyanine dyes with nucleic acids. 4. Efficient 5-fluorescent labelling of oligonucleotides with monomethyne pyrylium cyanine dye, Cyan 39 / S.M. Yarmoluk, A.M. Kostenko, O.S. Kornushyna, I.Y. Dubey // Биополимеры и клетка. — 1998. — Т. 14, № 1. — С. 82-86. — Бібліогр.: 16 назв. — англ.

A new system for fluorescent 5'-end labelling of oligonucleotides with cyanine dyes is proposed. Reaction of primary amine with pyrylium salt was used for the efficient conjugation of monomethyne pyrylium cyanine dye with 5'-aminoalkyl oligonucleotide with simultaneous conversion of the «b...