Overexpression of adaptor protein Ruk/CIN85 in mouse breast adenocarcinoma 4T1 cells induces an increased migration rate and invasion potential

• Дата: 2018
• Автори: Horak, I.R., Drobot, L.B., Borsig, L., Knopfova, L., Smarda, J.
• Формат: Стаття
• Мова: English
• Опубліковано: 2018
• Назва видання: Інститут молекулярної біології і генетики НАН України
• Теми: Structure and Function of Biopolymers
• Онлайн доступ: https://nasplib.isofts.kiev.ua/handle/123456789/154276
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Overexpression of adaptor protein Ruk/CIN85 in mouse breast adenocarcinoma 4T1 cells induces an increased migration rate and invasion potential / I.R. Horak, L.B. Drobot, L. Borsig, L. Knopfova, J. Smarda // Вiopolymers and Cell. — 2018. — Т. 34, № 4. — С. 284-291. — Бібліогр.: 33 назв. — англ.

Aim. To study the effect of adaptor protein Ruk/CIN85 overexpression on the dynamics of migration and Matrigel invasion as well as transendothelial migration of murine 4T1 breast adenocarcinoma cells. Methods. Dynamics of 4T1 cells migration/invasion was monitored in real time using the xCELLigence Real-Time Cell Analyzer (RTCA) DP Instrument equipped with a CIM-plate 16. Transendothelial migration (TEM) of 4T1 cells was performed through the layer of primary mouse lung endothelial cells seeded on gelatin-coated 24-well transwell inserts (8-μm pores).The two-tailed Student’s t-test for unequal variances was used for statistical analysis. Results. Ruk/CIN85-overexpression in 4T1 cells are indices a significantly increased motility, Matrigel invasiveness and migration through endothelial cells layer. Conclusions. The Ruk/CIN85 adaptor protein may play a potential role in the control of metastasis in vivo.