A role of expression level of reference and investigated genes in prostate tumors for qPCR analysis

• Published in: Вiopolymers and Cell
• Date: 2018
• Main Authors: Gerashchenko, G.V., Stakhovsky, E.O., Chashchina, L.I., Gryzodub, O.P., Kashuba, V.I.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2018
• Subjects: Genomics, Transcriptomics and Proteomics
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/154280
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: A role of expression level of reference and investigated genes in prostate tumors for qPCR analysis / G.V. Gerashchenko, E.O. Stakhovsky, L.I. Chashchina, O.P. Gryzodub, V.I. Kashuba // Вiopolymers and Cell. — 2018. — Т. 34, № 2. — С. 85-96. — Бібліогр.: 19 назв. — англ.

Aim. To determine the expression profiles of a set of cancer-associated genes in prostate tumors, using various normalization protocols (with 1, 2 and 4 reference genes) and to optimize a combination of reference genes to calculate the relative expression (RE) of the investigated genes in prostate c...