PPM1M and PRICKLE2 are potential tumor suppressor genes in human clear-cell renal cell carcinoma
Aim. To investigate the expression levels of PPM1M and PRICKLE2 in clear-cell renal cell carcinomas (ccRCC) and propose a mechanism leading to the expression changes in tumor. Methods. Analysis of GEO data, quantitative PCR (Q-PCR), bisulfite sequencing, methylation-specific PCR, deletion search. Re...
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| Date: | 2014 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Published: |
Інститут молекулярної біології і генетики НАН України
2014
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| Series: | Вiopolymers and Cell |
| Subjects: | |
| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/154299 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | PPM1M and PRICKLE2 are potential tumor suppressor genes in human clear-cell renal cell carcinoma / E.E. Rudenko, G.V. Gerashchenko, Y.V. Lapska, S.O. Vozianov, Y.M. Zgonnyk, V.I. Kashuba // Вiopolymers and Cell. — 2014. — Т. 30, № 3. — С. 229-233. — Бібліогр.: 11 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| Summary: | Aim. To investigate the expression levels of PPM1M and PRICKLE2 in clear-cell renal cell carcinomas (ccRCC) and propose a mechanism leading to the expression changes in tumor. Methods. Analysis of GEO data, quantitative PCR (Q-PCR), bisulfite sequencing, methylation-specific PCR, deletion search. Results. We found that the PRICKLE2 expression was down-regulated in 83 % of samples. Decreased expression of PPM1M was shown in 33.3 % of ccRCC samples. The promoters of PPM1M and PRICKLE2 were not methylated, and no deletions were found in their sequences. Conclusions. Our data suggest that PRICKLE2 and PPM1M might be candidates for the tumor suppressor genes in ccRCC. |
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