Expression and purification of full-length Alanyl-tRNA-synthetase from Thermus thermophilus HB27

• Published in: Вiopolymers and Cell
• Date: 2018
• Main Authors: Rybak, M.Yu., Priss, A.E., Gudzera, O.I., Kovalchuk, A.O., Kryklyvyi, I.A., Tukalo, M.A.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2018
• Subjects: Structure and Function of Biopolymers
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/154370
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Expression and purification of full-length Alanyl-tRNA-synthetase from Thermus thermophilus HB27 / M.Yu. Rybak, A.E. Priss, O.I. Gudzera, A.O. Kovalchuk, I.A. Kryklyvyi, M.A. Tukalo // Вiopolymers and Cell. — 2018. — Т. 34, № 6. — С. 435-444. — Бібліогр.: 29 назв. — англ.

Aim. To gain insight into structural and functional properties of alanyl-tRNA,synthetase (AlaRS), we genetically engineered constructs for expression and purification of full-length AlaRS and checked its activity in aminoacylation assays. Methods. The genomic DNA for the AlaS gene from the T. thermo...