Identification of a novel S6K1 splice variant coding for the p60-S6K1 isoform

• Published in: Вiopolymers and Cell
• Date: 2019
• Main Authors: Zaiets, I.V., Holiar, V.V., Filonenko, V.V.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2019
• Subjects: Structure and Function of Biopolymers
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/154396
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Identification of a novel S6K1 splice variant coding for the p60-S6K1 isoform / I.V. Zaiets, V.V. Holiar, V.V. Filonenko // Вiopolymers and Cell. — 2019. — Т. 35, № 2. — С. 99-106. — Бібліогр.: 24 назв. — англ.

Aim. To identify a splicing mRNA of S6K1 kinase specific for the p60-S6K1 isoform alone. Methods. RT-PCR, DNA sequencing, Western blotting. Results. RT-PCR analysis of total RNA extracted from MCF-7 cells and subsequent DNA sequencing revealed a novel S6K1 splice variant that possesses additional ex...