Elaboration of new method of enzyme adsorption on silicalite and nano beta zeolite for amperometric biosensor creation

• Datum: 2014
• Hauptverfasser: Soldatkin, O.O., Ozansoy Kasap, B., Akata Kurc, B., Soldatkin, A.P., Dzyadevych, S.V., El’skaya, A.V.
• Format: Artikel
• Sprache: English
• Veröffentlicht: Інститут молекулярної біології і генетики НАН України 2014
• Schriftenreihe: Вiopolymers and Cell
• Schlagworte: Molecular and Cell Biotechnologies
• Online Zugang: https://nasplib.isofts.kiev.ua/handle/123456789/154426
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Zitieren: Elaboration of new method of enzyme adsorption on silicalite and nano beta zeolite for amperometric biosensor creation / O.O. Soldatkin, B. Ozansoy Kasap, B. Akata Kurc, A.P. Soldatkin, S.V. Dzyadevych, A.V. El’skaya // Вiopolymers and Cell. — 2014. — Т. 30, № 4. — С. 291-298. — Бібліогр.: 24 назв. — англ.

Aim. Optimization of a new method of enzyme immobilization for amperometric biosensor creation. Methods. The amperometric biosensor with glucose oxidase immobilized on zeolites as bioselective elements and platinum disk electrode as transducers of biochemical signal into the electric one was used in the work. Results. The biosensors based on glucose oxidase adsorbed on zeolites were characterized by a higher sensitivity to glucose and a better inter-reproducibility. The best analytical characteristics were obtained for the biosensors based on nano beta zeolite. It has been found that an increase in the amount of zeolite on the surface of amperometric transducer may change such biosensor parameters as sensitivity to the substrate and duration of the analysis. Conclusions. The proposed method of enzyme immobilization by adsorption on zeolites is shown to be quite promising in the development of amperometric biosensors and therefore should be further investigated.