Toxic effect of C₆₀ fullerene-doxorubicin complex towards tumor and normal cells in vitro

• Дата: 2014
• Автори: Prylutska, S.V., Didenko, G.V., Potebnya, G.P., Bogutska, K.I., Prylutskyy, Yu.I., Ritter, U., Scharff, P.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2014
• Назва видання: Вiopolymers and Cell
• Теми: Bioorganic Chemistry
• Онлайн доступ: https://nasplib.isofts.kiev.ua/handle/123456789/154553
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Toxic effect of C₆₀ fullerene-doxorubicin complex towards tumor and normal cells in vitro / S.V. Prylutska, G.V. Didenko, G.P. Potebnya, K.I. Bogutska, Yu.I. Prylutskyy, U. Ritter, P. Scharff // Вiopolymers and Cell. — 2014. — Т. 30, № 5. — С. 372-376. — Бібліогр.: 25 назв. — англ.

Creation of new nanostructures possessing high antitumor activity is an important problem of modern biotechnology. Aim. To evaluate cytotoxicity of created complex of pristine C₆₀ fullerene with the anthracycline antibiotic doxorubicin (Dox), as well as of free C₆₀ fullerene and Dox, towards different cell types – tumor, normal immunocompetent and hepatocytes. Methods. Measurement of size distribution for particles in C₆₀ + Dox mixture was performed by a dynamic light scattering (DLS) technique. Toxic effect of C₆₀+ Dox complex in vitro towards tumor and normal cells was studied using the MTT assay. Results. DLS experiment demonstrated that the main fraction of the particles in C₆₀+ Dox mixture had a diameter in the range of about 132 nm. The toxic effect of C₆₀ + Dox complex towards normal (lymphocytes, macrophages, hepatocytes) and tumor (Ehrlich ascites carcinoma, leukemia L1210, Lewis lung carcinoma) cells was decreased by ~10–16 % and ~7–9 %, accordingly, compared with the same effect of free Dox. Conclusions. The created C₆₀ + Dox composite may be considered as a new pharmacological agent that kills effectively tumor cells in vitro and simultaneously prevents a toxic effect of the free form of Dox on normal cells.