Methylene blue: an alternative, multi-purpose stain for detection, analysis and isolation of nucleic acids

• Published in: Биополимеры и клетка
• Date: 1997
• Main Authors: Johnson, K.M., Hanekamp, T., Stayton, M.M.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 1997
• Subjects: Методы
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/155205
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Methylene blue: an alternative, multi-purpose stain for detection, analysis and isolation of nucleic acids / К.M. Johnson, T. Hanekamp, M.M. Stayton // Биополимеры и клетка. — 1997. — Т. 13, № 3. — С. 250-253. — Бібліогр.: 8 назв. — англ.

A series of experiments was performed utilizing Methylene Blue (MeB) in place of the intercalating dyes ethidium bromide (EtBr) and acridine orange (AO) to stain, visualize, and isolate DNA and RNA. MeB proved to be superior to the other dyes for several purposes: 1) visualization of glyoxalated (ch...