Some properties of MS2-induced bacterial mutants;
Some genotype and phenotype properties of MS2-induced being granular and lysing Escherichia coli mutants are described –form of colonies, cells form and size, susceptibility to lytic agents, carbohydrates utilization, antigenic properties, Gram staining, patterns of growth and division. The numerous...
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| Дата: | 1998 |
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Інститут молекулярної біології і генетики НАН України
1998
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| Цитувати: | Some properties of MS2-induced bacterial mutants; / T.P. Pererva // Биополимеры и клетка. — 1998. — Т. 14, № 3. — С. 231-237. — Бібліогр.: 29 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1859789531153170432 |
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| author | Pererva, T.P. |
| author_facet | Pererva, T.P. |
| citation_txt | Some properties of MS2-induced bacterial mutants; / T.P. Pererva // Биополимеры и клетка. — 1998. — Т. 14, № 3. — С. 231-237. — Бібліогр.: 29 назв. — англ. |
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| container_title | Биополимеры и клетка |
| description | Some genotype and phenotype properties of MS2-induced being granular and lysing Escherichia coli mutants are described –form of colonies, cells form and size, susceptibility to lytic agents, carbohydrates utilization, antigenic properties, Gram staining, patterns of growth and division. The numerous aberrations from wild type bacteria found in these mutants are supposed to be due not to responsible loci mutations but to accompany structural rearrangements in cell membrane components.
Описано деякі генотипові та фенотипові властивості MS2-індукованих мутантів Е. coli зернистого та лізуючого типів – форма колоній, форма, та об'єм клітин, чутливість до літичних агентів, утилізація вуглеводів, антигенні властивості, забарвлення по Грому, специфіка росту та ділення. Припускається, що численні відхилення від властивостей бактерій дикого типу зумовлені не мутаціями у відповідних генах, а пов'язані зі структурною перебудовою компонентів клітинної мембрани.
Описаны некоторые генетические и фенотипические свойства М82-индуцирова.нных мутантов Е. coli зернистого и лизирующего типов – форма колоний, форма и объем клеток, чувствительность к литическим агентам, утилизация углеводов, антигенные свойства, окраска по Граму, тип роста и деления. Предполагается, что многочисленные отклонения от свойств бактерий дикого типа обусловлены не мутациями в соответствующих генах, а связаны со структурными перестройками компонентов клеточных мембран.
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I S S N 0233-7657, Биополимеры и клетка. 1998. Т. 14. № 3
В И Р У С Ы И К Л Е Т К А
Some properties of MS2-induced bacterial mutants;
Tamara P. Pererva
Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine
150 Zabolotnogo str., 252143 , Kyiv, Ukraine
Some genotype and phenotype properties of MS2-induced being granular and lysing Escherichia coli
mutants are described — form of colonies, cells form and size, susceptibility to lytic agents, carbohydrates
utilization, antigenic properties, Gram staining, patterns of growth and division. The numerous aberrations
from wild type bacteria found in these mutants are supposed to be due not to responsible loci mutations
but to accompany structural rearrangements in cell membrane components.
Introduction. We have earlier described the induction
of MS2-resistant mutants developing in the offspring
of an Escherichia coli AB259 Hfr3000 (E. coli 3000)
cell following this phage infection [1 ]. Such phage-
induced mutants keep in generat ions the MS2-re-
sistance marker accompanied, however, by segre
gation of new mutant types; some of them belong to
intermediate forms, two of them being final ones [2].
T h e segregation of some new forms from a
primary mutant strain suggests the presence of in
stability in a mutant cells, such a fact being a striking
feature of such mutants comparing to spontaneous
ones and suggesting these forms to be phage-induced
ones. Having cloned the chromosomal DNA fragment
of one of these mutan ts on a non-replicating Ap-
fragment f3 ] we were able to suppose that obtained
experimental system may be used for s tudying of
RNA phages-host intractions and possibility of some
type of integration of these phages RNA or its pari:
with E. coli DNA. These investigations have been also
continued using hybridizat ion experiments with a
preparation of MS2 cDNA | 4 ] and by a genetic
transfer of the mutant properties into the A-phage
chromosome [5 ] .
This paper describes some properties of cells of
two final MS2-induced mutants demonstra t ing that
their numerous aberra t ions from wild type bacteria
are not due to mutat ions having place in corres
ponding genes but may be connected with some
structural disturbances of mutan t cell wall.
Materials and Methods . Bacterial strains. E. coli
© T. P. PERERVA, 1998
strains used in this s tudy a re AB259 Hfr3000 (E. coli
3000) (Institute of Molecular Biology, Russian Aca
demy of Sciences, Moscow), K12 (A) and CR63
(Insti tute of Microbial Biochemistry and Physiology,
Russian Academy of Sciences, Pushchino-on-Oka) ; E.
coli CR63 Ah r has been obtained in our laboratory by
selection of cells resis tant against h -mutan t of A-
phage.
Bacteriophages. In our exper iments we worked
with such phages as MS2 (Insti tute of Molecular
Biology, Russian Academy of Sciences, Moscow), A
(Insti tute of Microbial Biochemistry and Physiology,
Russian Academy of Sciences, Pushchino-on-Oka) ,
0 x 2 , O x 2 h l 0 , O x 2 h l 2 and T u l b [6] kindly provided
by Prof. U. Henning; h - and hh-A-mutants were
obtained in our laboratory by spontaneous mutants
selection in E. coli CR63 and E. coli CR63 Ah r cells,
respectively. A wild type P I s train as well as T7
phage were taken from the collection of our Institute.
Culture media. Both liquid (LB) and agar-con-
taining (LA) (0.8 and 1.8 %) L-medium was used for
bacteria growth. In some exper iments our strains grew
also in a liquid medium and on an agar medium
containing aminopeptide (APB and АРА, respec
tively) .
Assay methods. An ant iphage serum was obtained
as described by Adams [7 ] . Electron microscopic
preparat ions were prepared and investigated accor
ding to Bespalova et ah [8 J.
Results. MS2-induced mutan t s , both a granular
and a lysing ones, used in this investigation have
been compared to an original s t ra in , E. coli 3000; we
have studied several markers such as forms of
231
PERERVA Г. p.
colonies, cells forms and sizes, their susceptibility to
lysing agents, ability to carbohydrates utilization,
inactivation by a serum against heated wild type
bacteria, Gram staining. The data obtained have been
interpretated taking into consideration two mutants
growth in liquid and solid media as well as their
thermosusceptibility and auxotrophy.
Forms of colonies. G r a n u l a r t y p e . The
colonics of this type have often more dense centers
arid t ransparant wavy edges (Fig. 1). When such
colonies are kept at Petri dishes during a ra ther long
time they grow extensively and become branching, a
part of cells growing into the agar medium. Contrary
to wild type «homogenous» colonies (Fig. 2) , these
ones are of granular s t ructure.
L y s i n g t y p e . T h e cells of this type mutant
give usually colonies sur rounded by lysis zone on
confluent lawns formed by granular type mutan t
bacteria (Fig. 3) . When growing on lawns of wild type
bacteria lysing ones form separate colonies causing,
however, no lysis. The lysing bacteria put on a usual
solid medium without preformed lawn form colonies
of ra ther contrast edges being denser comparing to
wild type colonies. These colonies are homogenous;
however, during further passages a part of colonies
began to form large branches of creeping growth (Fig.
4) . Using the lens it becomes evident that the
structure of these colonies, especially of young ones,
is a little granular; however, such a granular ap
pearance is seen only on margins of colonies, their
granularity being denser comparing to granular mu
tants one.
Mutant cells form and size. G r a n u l a r t y
p e c e l l s . Electron microscopic investigations
detect that granular type colonies (Fig. 5) contain a
mixture of alive cells and of dead ones filled with
l u m p s . A pa r t of d e a d cells a re non-d iv ided
filamentous forms, another cells of the same colony
are markedly shortened comparing to wild type ones.
A lot of cells are of curved shapes and possess
spherical areas including a part of individual cell
length. Contrary to wild type bacteria the granular
cells possess no F-pili but keep yet their flageila.
L y s i n g t y p e c e l l s . Any lysing type
colony contains no dead cells contrary to granular
type one. So it contains no curved forms and in
flations. Its cells are several times larger comparing to
wild type ones, their sizes being dependent on culture
age and its cultivation conditions. Old culture without
aeration contain longer non-divided forms; at the
same time, young cells being in active division process
present shorter forms and show a marked tendency to
form chains (Fig. 6) . A striking feature of lysing type
cells growth is cells stiking by their side surfaces
followed by conglomerations formation (Fig. 7) being
evidently the cause of pellet appearance on the bottom
of liquid cultures. Electron microscopic study of lysing
type cells does not usually detect both F-pili and
flageila; however, some cells restore their flageila
after numerous passages. Another interesting marker
Fig. 1. Colonies of a granular type mutant
Fig. 2. Colonies of wild type cells
232
SOME P R O P E R T I E S O F M S 2 - I N D U C E D BACTERIAL MUTANTS
Fig. 3. Colonies of a lysing type mutant on a lawn developed by granular type cells
Fig. 4. Colonies of a lysing хуэе mutant forming some branches of creeping growth
Fig. 5. Cells of a granular type mutant
Fig. 6. Chains of cells formed by a lysing type mutant
found in these cells is a specific structure similar to
nucleoid in actively dividing E. coli cells (Fig. 8 ) .
Mutant cells reproduction in the liquid medium.
G r a n u l a r t y p e c e l l s . While cultivating in
the liquid medium the granular type cells are in
retard comparing to wild type ones in their growth
rate and optical density level. Such a difference
strikes especially if the the APB is used for bacteria
cultivation, the LB medium increasing this mutant
division rate and its optical density values.
L y s i n g t y p e . Cont rary to the granular type
mutant , the lysing type one possesses growth advan
tages comparing to wild type bacteria. During the
lag-period the mutant culture optical density values in
liquid medium increase more intensively comparing to
wild type cultures, the mutan ts transit ion to the
stat ionary phase having place also at higher culture
density than such a transition by any wild type
culture. Besides, the lysing type cells inoculated
together with wild type ones form separate colonies on
233
PERERVA Т. F.
Fig. 7. Cell agglomerations of a lysing type ramant
Fig. 8. Nucleoids in lysing mutant cells
a confluent lawn of wild type cells. Such a peculiarity
is a very interesting one because any other E. coll
mutants in mixed inocula with wild type cells do not
form separate colonies. T h e lysing mutant selective
advantage comparing to wild type bacteria correlates
with its growth rate and its other growth characters
in the liquid medium.
Susceptibility to lysing agents. G r a n u l a r
m u t a n t с e 1 1 s. As we have already mentioned
above this mutant cells are susceptible to a lytical
agent containing by a secret of the lysing mutant . The
susceptibility marker correlates with higher sensitivity
to chloroform and lysozyme. T h e mutant cells do not
grow on solid media in the area of a spot containing
0.2 % chloroform. Another lysing agent, lysozyme.
causes the complete cell lysis when used in concen
trations necessary to obtain E. с oil spheroplasts
preparation: 0.02 ml of 0.2 % enzyme solution
supplemented with 0.04 ml of 4 % EDTA are enough
for the complete degradat ion of 10 ml of mutant cells
suspension, the cells concentration being 1 • 1 0 9 / m l .
Besides, a part of mutan t cells are lysed spon
taneously while keeping in the liquid medium. The
lawns formed by such mutants on the solid medium
are often covered by small plaques containing no
infectious phage particles.
L y s i n g m u t a n t c e l l s . These mutant
cells demonstra te no increased sensitivity to lytical
substances; however, some autolysis pat terns have
been demonstrated in cultures having been kept too
long on Petri dishes; such a phenomenon is seen
because of the bacterial lawn center becoming clearer.
Carbohydrates utilization. G r a n u l a r t y
p e c e l l s. Such cells do not decompose lactose,
mannitol , maltose, xylose, dulcitol, rhamnose , sorbi
tol, and arabinose; they, however, ferment glucose up
to acid product and cause also a faint sucrose
fermentation.
L y s i n g m u t a n t c e l l s . Thei r cultures
are active glucose decomposers forming the acid
product during 24 hours and also of maltose and
arabinose, the acid appear ing on the fourth day of
fermentation. All the other carbohydrates named for
the granular type cells are not decomposed by the
lysing mutant .
G r a m staining. Both granular and lysing mutant
cultures a re Gram-s ta ined in a mosaic manner .
Antigenic properties. G r a n u l a r t y p e
c e l l s . These cells a re not agglutinated by a rabbit
ant iserum raised against hea ted wild type cells, the
original culture is agglutinated by the same serum
diluted up to 1 : 800—1 : 1600.
L y s i n g t y p e c e l l s . The lysing type
cells are agglutinated by the serum mentioned above
diluted to 1 : 50—1 : 100.
Mutants susceptibility to some coliphages. The
mutants susceptibility to some E. coli infecting phages
has been determined by the spot-test method using
infectious inocula containing 1 0 9 — 1 0 I J part icles/ml.
Both mutants have been demonst ra ted to be sensitive
to such phages as 0 x 2 , O x 2 h l 0 , O x 2 h l 2 , T u l b and
PI and not sensitive to A and T7 phages. Both
MS2-induced E. coli mutants are also sensitive to h-
and hh-mutants of the A-phage.
234
Presence of conditionally lethal mutations. Both
granular and lysing mutants do not demonst ra te any
auxotrophy or thermolability marker and form their
colonies in salts-containing minimal medium and at
42 °С,
Rev er lability. No mutant form has reverted to the
original morphologic type presented by smooth non
granular colonies without lysis zones possessing stan
dard morphology including original antigenic s t ruc
ture, susceptibility to coliphages, and carbohydrates
utilization; sometimes, however, the sporadic flageila
restoration has place by a lysing type mutant .
Discussion. The phenotypic markers of MS2-
induced granular and lysing type mutants described
in this paper may be divided in two groups. T h e first
group of markers includes Gram staining, suscep
tibility to lysing agents and coliphages, carbohydrate
utilization, pooverty of ant igens composition, complete
F-pili loss and partial loss of flageila, form and size
of mutant cells and of colonies formed by them. T h e
second markers group concerns mutant cells growth
and division properties. T h e analysis of both mutant
groups permits to found interrelations between these
two groups as well as between separate odd-looking
properties of MS2-induced mutan ts .
Among the first group mutan t characters only the
loss of F-pili described in our previous papers [1 , 2 |
is seeming to be due to mutat ional events in the
region of chromosome-integrated F-factor. Develop
ment of the other mutant characters including mul
tiple disturbance of carbohydrates utilizations can not
be explained by the F-factor mutat ions and is pro
bably the result of some other events.
Having used LPS-specific phages T7 and P I we
demonstrate the both mutants — the granular mutant
and the lysing one — are resistant against T 7 being.,
however, PI-suscept ible bacteria. T h e development of
T7~resistance suggests the dis turbance of LPS struc
ture by both mutants [9—11 ], at least of that part of
LPS which is included into forming of phage receptors
on the surface of bacterial cell. As to P I phage it is
known to possess a wide host range, its bacterial
receptor being a core oligosaccharide; this s tructure is
shown to be relatively invariable by Enterobacteria.
Disturbances of LPS structure like the carbo
hydra te chains shortening may result in partial sub
stitution of LPS by phospholipids; such a substi tution
increases the cell permeability for hydrophobic sub
stances accompanied by defective cell membrane com
ponents rear rangement (12—13] . Some holes may
appear in this membrane dependent on the properties
of such rearrangements causing mosaic Gram-sta in ing
and increased susceptibility to lysing agents . Mo
reover some LPS-defects such as the partial absence
S O M E PROPERTIES' OF M S 2 - I N D U C E D BACTERIAL MUTANTS
of O-side chains causes cell deformations, developing
of cell projections [14 ] and forming of R- type colonies
[15—17] as well as antigenic s t ructure poorness.
T h e list of markers developing in MS2-induced
mutants parallely with dis turbed LPS forming may be
supplemented also by the flageila absence in the
lysing type mutant . The i r sporadical restoration follo
wed by the development of creeping growth branches
seems to correlate with slight restorat ion of maltose
and arabinose fermentation by this mutant ; such a
fact is in good agreement with a point of view [18]
that the phosphotransferase system (PTS) responsible
for the carbohydrates t ranspor t regulates also bac
terial flageila proteins synthesis controlling the mo
tility expression.
Analysing the loss of the carbohydrates utili
zation markers by both mutan t s we must take into
consideration that this phenomenon is of the multiple
na ture ; so it can be likely due to mutat ions having
pleiotropic effect r a the r than to mutat ions in the genes
encoding individual permeases and scuttered along
the whole E. coli chromosome [19] .
One of the systems theoretically capable to block
the carbohydrates t ransport is presented by porins
being the outer membrane proteins [20 J.
In our experimental system at least two main
proteins of our granular and lysing mutant outer
membrane — OmpA a n d OmpC — keep their pre
sence and function; such a reality is suggested by
mutants susceptibility to phages 0 x 2 (OmpA-specific
receptor) , Ox2hlO and O x 2 h l 2 (specific for OmpA
and OmpC proteins) as well as to T u l b (specific for
OmpC) [6 ]. Simultaneously, the LamB function of
mutan t cells is damaged (absence of maltose uti
lization by the granular mutan t , its very faint fer
mentat ion by the lysing mutan t and absence of A
susceptibility for both mu tan t s ) . However, this protein
is evidently present in the outer membrane structure
of both mutants judging by their susceptibility to h-
and hh-hos t range A mutants . In such a way, the
preferencial keeping of principal outer membrane
proteins activities as well as scattered distribution of
their coding genes along the E. coli chromosome do
not permit to think these genes to be responsible for
the mass loss of ca rbohydra te utilization in MS2-
induced mutants .
T h e most marked pleiotropic effect influencing
the bacterial carbohydra tes metabolism is known to
occur due to some defects of the carbohydra te t rans
fer by PTS [18, 2 1 ] , but in our case such a
presumption is little possible because of several rea
sons.
First of all, in a n y case of PTS mutations
accompanied by the pleiotropic effect the complete
235
PERERVA Т. P.
loss of the glucose utilization marker is seen, the
glucose fermentation being kept by the granular and
lysing type mutan t s . Second, the glucose fermentation
observed in our granular mutan t has been never
found in any E. coli PTS mutant .
So the analysis of the cell damages and of the
carbohydrate utilization disturbancies by MS2-in-
duced mutants made above does not permit to connect
them with some mutat ions in the genes controlling
surface binding proteins, outer membrane proteins,
and bacterial P T S . However, the main objection
against the presence of mutat ions in all these three
genes groups is the absence of auxotrophy as well as
of thermosensibili ty in both granular and lysing type
mutants . First, such a fact does not correlate with
typical properties of mutan ts with damaged car
bohydrate t ransport and utilization [21 ]. Second, the
so-called «membrane» E. coli mutants are well-known
to be thermosensitive (ts) ones and vice versa about
80 % of E, coli ts mutants a re membrane ones [13] ,
Taking all these da ta into account it is reasonable to
discuss also other markers of the MS2-induced mu
tants and their possible dependence on cell functional
damages.
The MS2-induced mutan ts studied here present a
complete system of mutants segregated from a pri
mary MS2-resistant mutan t through a series of inter
mediate forms [1 , 2 ] . T h e change of F-pili state
beginning from the function loss by the pr imary-
resistant mutant up to their structural disappearance
by the granular and lysing mutan t s accompanied by
the absence of reversions in segregants permit to
suggest the segregation of ail the derivative forms to
be due to deletions occurred in the mutant region
localized in the F-factor. T h e aggravation of the
primary mutation localized there is followed by seg
regants growth function damage becoming more faible
in the granular type mutan t and markedly accelerated
in the lysing type mutant . T h e cell division process is
damaged by both mutan ts ; however, the culture of the
granular type mutant contains a lot of dead fila
mentous cells; the culture of the lysing type mutant
is, on the contrary, presented by larger cells being
alive, homogenous, and containing more than one
nucleoid; such cells form often chains and conglo
merations. T h e bacterial growth ra te and division are
known to be dependent on their DNA replication
controlled on the initiation level [22] . All the mutants
of our experimental system originate from an E. coll
Hfr3000 cell carrying the F-factor integrated into the
cellular DNA. In such a case the cell replication may
be initiated both from the chromosomal oriC and from
the oriF [23] . All the mutant events being deeply
connected with the F-factor functions, it is possible to
assert with a ra ther high probability the cellular DNA
replication to be initiated from the oriF. According to
the data concerning the levels of growth and division
damages in the granular mutan t as well as the
nucleoids number and size in the lysing one pos
sessing the immense DNA replication rate accom
panied by cell division re tardat ion, the damages of
oriF s tructure and functions a re very considerable.
During last two decades a lot of information was
accumulated concerning the cell wall role in the DNA
replication and the replicon association with mem
brane proteins [24—26] . T h e molecular masses of
these proteins are 55—80 kDa according to different
authors [27, 2 8 ] , their principal function being the
bridging between DNA and outer membrane through
the peptidoglycan layer. Dur ing such a bridging a new
structure appears unit ing the outer cell membrane ,
mureine and replication origin site; such a structure
regulates the DNA replication and its correlation with
cell division. It is demonst ra ted with the E. coli oriC
model the initiation region is to possess a specific
sequence as long as 463 b . p. to bind the membrane
protein [28] .
So the replication origin s t ructure is of the
greatest importance for the DNA-membrane complex
formation, that is why all the mutat ions and struc
tural changes occurred in the origin region of the
MS2-induced mutants make such a process more
difficult. The overcoming of these difficulties may, in
its turn , be accompanied by damage and rear
rangements of cell membranes components due to
irregular localization of the large protein molecule
penetrat ing all the cell membrane layers . T h e systems
assuring the t ransport of differently directed streams
of subst ra ta and of cell metabolic products are stron
gly dependent on s tructure and function state of the
cell membrane . Its defects develop a lot of aberrations
similar to these ones observed for MS2-induced
mutants . So the mutants described in this paper are
an exellent and perspective model permitting the
deepest investigations concerning the effect of the
initiation region on the cell wall, cell growth and
division, and total cell metabolism. Such a t rend
might have elaborated a new approach allowing to
unders tand neoplastic transformation mechanism and
to extend these processes modelling from lower euka-
ryotic organisms [29 ] up to prokaryotes .
Acknowledgements. T h e au thor is indebted to Dr
S. Bobrovnik (Volodymyr Palladin Insti tute of Bio
chemistry, National Academy of Sciences of Ukraine)
for electron microscopic photoes and to Dr Ella
Zherebtsova having obtained a serum against E. coli
cells and helped me to prepare this manuscript for
publication.
236
S O M E P R O P E R T I E S O F M S 2 - I N D U C E D BACTERIAL MUTANTS
Т. П. Перерва
Деякі властивості М82-індукованих бактеріальних мутантів
Резюме
Описано деякі генотипові та фенотипові властивості MS2-
індукованих мутантів Е. coli зернистого та лізуючого ти
пів — форма колоній, форма, та об'єм клітин, чутливість до
літичних агентів, утилізація вуглеводів, антигенні власти
вості, забарвлення по Грому, специфіка росту та ділення.
Припускається, що численні відхилення від властивостей бак
терій дикого типу зумовлені не мутаціями у відповідних
генах, а пов'язані зі структурною перебудовою компонентів
іслітинної мембрани.
Т. 77. Перерва
Некоторые свойства М82-инцуцированных бактериальных
мутантов
Резюме
Описаны некоторые генетические и фенотипические свойства
М82-индуцирова.нных мутантов Е. coli зернистого и лизирую-
щего типов — форма колоний, форма и объем клеток, чувст
вительность к литическим агентам, утилизация углеводов,
антигенные свойства, окраска по Граму, тип роста и деления.
Предполагается, что многочисленные отклонения от свойств
бактерий дикого типа обусловлены не мутациями в соответ
ствующих генах, а связаны со структурными перестройками
компонентов клеточных мембран.
REFERENCES
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237
http://Chem.-1976.-251
|
| id | nasplib_isofts_kiev_ua-123456789-155384 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 0233-7657 |
| language | English |
| last_indexed | 2025-12-02T10:59:44Z |
| publishDate | 1998 |
| publisher | Інститут молекулярної біології і генетики НАН України |
| record_format | dspace |
| spelling | Pererva, T.P. 2019-06-16T17:14:21Z 2019-06-16T17:14:21Z 1998 Some properties of MS2-induced bacterial mutants; / T.P. Pererva // Биополимеры и клетка. — 1998. — Т. 14, № 3. — С. 231-237. — Бібліогр.: 29 назв. — англ. 0233-7657 DOI: http://dx.doi.org/10.7124/bc.0004D2 https://nasplib.isofts.kiev.ua/handle/123456789/155384 Some genotype and phenotype properties of MS2-induced being granular and lysing Escherichia coli mutants are described –form of colonies, cells form and size, susceptibility to lytic agents, carbohydrates utilization, antigenic properties, Gram staining, patterns of growth and division. The numerous aberrations from wild type bacteria found in these mutants are supposed to be due not to responsible loci mutations but to accompany structural rearrangements in cell membrane components. Описано деякі генотипові та фенотипові властивості MS2-індукованих мутантів Е. coli зернистого та лізуючого типів – форма колоній, форма, та об'єм клітин, чутливість до літичних агентів, утилізація вуглеводів, антигенні властивості, забарвлення по Грому, специфіка росту та ділення. Припускається, що численні відхилення від властивостей бактерій дикого типу зумовлені не мутаціями у відповідних генах, а пов'язані зі структурною перебудовою компонентів клітинної мембрани. Описаны некоторые генетические и фенотипические свойства М82-индуцирова.нных мутантов Е. coli зернистого и лизирующего типов – форма колоний, форма и объем клеток, чувствительность к литическим агентам, утилизация углеводов, антигенные свойства, окраска по Граму, тип роста и деления. Предполагается, что многочисленные отклонения от свойств бактерий дикого типа обусловлены не мутациями в соответствующих генах, а связаны со структурными перестройками компонентов клеточных мембран. The author is indebted to Dr S. Bobrovnik (Volodymyr Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine) for electron microscopic photoes and to Dr Ella Zherebtsova having obtained a serum against E. coli cells and helped me to prepare this manuscript for publication. en Інститут молекулярної біології і генетики НАН України Биополимеры и клетка Вирусы и клетка Some properties of MS2-induced bacterial mutants; Деякі властивості М82-індукованих бактеріальних мутантів Некоторые свойства М82-инцуцированных бактериальных мутантов Article published earlier |
| spellingShingle | Some properties of MS2-induced bacterial mutants; Pererva, T.P. Вирусы и клетка |
| title | Some properties of MS2-induced bacterial mutants; |
| title_alt | Деякі властивості М82-індукованих бактеріальних мутантів Некоторые свойства М82-инцуцированных бактериальных мутантов |
| title_full | Some properties of MS2-induced bacterial mutants; |
| title_fullStr | Some properties of MS2-induced bacterial mutants; |
| title_full_unstemmed | Some properties of MS2-induced bacterial mutants; |
| title_short | Some properties of MS2-induced bacterial mutants; |
| title_sort | some properties of ms2-induced bacterial mutants; |
| topic | Вирусы и клетка |
| topic_facet | Вирусы и клетка |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/155384 |
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