Transfer of gene conferring herbicide bialaphos resistance into buckwheat plants

• Date: 1997
• Main Authors: Rubtsova, M.A., Levenko, B.A., Taranenko, L.K., Stekhin, I.N., Sayaz, L.I.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 1997
• Series: Биополимеры и клетка
• Subjects: Генно-инженерная биотехнология
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/155645
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Transfer of gene conferring herbicide bialaphos resistance into buckwheat plants / M.A. Rubtsova, B.A. Levenko, L.K. Taranenko, I.N. Stekhin, A.I. Sayaz // Биополимеры и клетка. — 1997. — Т. 13, № 5. — С. 416-418. — Бібліогр.: 14 назв. — англ.

Bar gene conferring resistance to herbicide bialaphos (phmphinothricin) was cloned from Streptomyces hygroscopicus. Bar gene under 35S promoter of cauliflower mosaic virus was introduced in binary pBin19 vector and constructed plasmid was transferred into Agrcbacterium tumefaciens strain. Conditions of genetic transformation of cultivated buckwlwat and interspecific hybrid Fagopirum esculentum X F. tataricum were worked out. Buckwheat explants were inoculated by the strain with a plasmid carrying bar gene nearby NPTII gene. Molecular analysis of 8 regenereted plants that were selected for kanamycin resistance was performed. 5 plants gave positive signal by dol- and Southern hybridization using as a probe DNA fragment with bar gene that is an evidence of integration of bar gene into plant genome. Transformed plants grow and rooted at Basta concentrations in the medium, that totally inhibited nontransformed plants.