Modelling and measurements of fibrinogen adsorption on positively charged microspheres
Adsorption of fibrinogen on positively charged microspheres was theoretically and experimentally studied. The structure of monolayers and the maximum coverage were determined by applying the experimental measurements at pH = 3.5 and 9.7 for NaCl concentration in the range of 10⁻³ - 0.15 M. The maxim...
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| Veröffentlicht in: | Condensed Matter Physics |
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| Datum: | 2016 |
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | Englisch |
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Інститут фізики конденсованих систем НАН України
2016
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| Online Zugang: | https://nasplib.isofts.kiev.ua/handle/123456789/155785 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Modelling and measurements of fibrinogen adsorption on positively charged microspheres / P. Zeliszewska, A. Bratek-Skicki, Z. Adamczyk, M. Ciesla // Condensed Matter Physics. — 2016. — Т. 19, № 1. — С. 13801: 1–14. — Бібліогр.: 42 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862739189414494208 |
|---|---|
| author | Zeliszewska, P. Bratek-Skicki, A. Adamczyk, Z. Ciesla, M. |
| author_facet | Zeliszewska, P. Bratek-Skicki, A. Adamczyk, Z. Ciesla, M. |
| citation_txt | Modelling and measurements of fibrinogen adsorption on positively charged microspheres / P. Zeliszewska, A. Bratek-Skicki, Z. Adamczyk, M. Ciesla // Condensed Matter Physics. — 2016. — Т. 19, № 1. — С. 13801: 1–14. — Бібліогр.: 42 назв. — англ. |
| collection | DSpace DC |
| container_title | Condensed Matter Physics |
| description | Adsorption of fibrinogen on positively charged microspheres was theoretically and experimentally studied. The structure of monolayers and the maximum coverage were determined by applying the experimental measurements at pH = 3.5 and 9.7 for NaCl concentration in the range of 10⁻³ - 0.15 M. The maximum coverage of fibrinogen on latex particles was precisely determined by the AFM method. Unexpectedly, at pH = 3.5, where both fibrinogen molecule and the latex particles were positively charged, the maximum coverage varied between 0.9 mg m⁻² and 1.1 mg m⁻² for 10⁻² and 0.15 M NaCl, respectively. On the other hand, at pH = 9.7, the maximum coverage of fibrinogen was larger, varying between 1.8 mg m⁻² and 3.4 mg m⁻² for 10⁻² and 0.15 M NaCl, respectively. The experimental results were quantitatively interpreted by the numerical simulations⁻² and 3.4 mg m⁻² for 10⁻² and 0.15 M NaCl, respectively. The experimental results were quantitatively interpreted by the numerical simulations.
Теоретично та експериментально дослiджено адсорбцiю фiбриногену на позитивно заряджених мiкросферах. Структура моношарiв i максимальне покриття були визначенi експериментально при pH = 3.5
i 9.7 для концентрацiї NaCl в дiапазонi 10⁻³ − 0.15 M. Максимальне покриття фiбриногену на частинках латексу було визначене прецизiйно методом мiкроскопу атомної сили (AFM). Несподiвано, що при
pH = 3.5, коли i фiбриноген i частинки латексу зарядженi позитивно, максимальне покриття змiнюється
мiж 0.9 мг м⁻²
i 1.1 мг м⁻²
для 10⁻²
i 0.15 M NaCl, вiдповiдно. При pH = 9.7 максимальне покриття фiбриногену є бiльшим i змiнюється вiд 1.8 мг м⁻²
до 3.4 мг м⁻²
для 10⁻²
i 0.15 M NaCl, вiдповiдно. Експериментальнi результати пiдтвердженi чисельним моделюванням.
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| first_indexed | 2025-12-07T20:07:43Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-155785 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1607-324X |
| language | English |
| last_indexed | 2025-12-07T20:07:43Z |
| publishDate | 2016 |
| publisher | Інститут фізики конденсованих систем НАН України |
| record_format | dspace |
| spelling | Zeliszewska, P. Bratek-Skicki, A. Adamczyk, Z. Ciesla, M. 2019-06-17T12:31:01Z 2019-06-17T12:31:01Z 2016 Modelling and measurements of fibrinogen adsorption on positively charged microspheres / P. Zeliszewska, A. Bratek-Skicki, Z. Adamczyk, M. Ciesla // Condensed Matter Physics. — 2016. — Т. 19, № 1. — С. 13801: 1–14. — Бібліогр.: 42 назв. — англ. 1607-324X DOI:10.5488/CMP.19.13801 arXiv:1603.02437 PACS: 82.70.Dd, 87.15A, 87.14.E https://nasplib.isofts.kiev.ua/handle/123456789/155785 Adsorption of fibrinogen on positively charged microspheres was theoretically and experimentally studied. The structure of monolayers and the maximum coverage were determined by applying the experimental measurements at pH = 3.5 and 9.7 for NaCl concentration in the range of 10⁻³ - 0.15 M. The maximum coverage of fibrinogen on latex particles was precisely determined by the AFM method. Unexpectedly, at pH = 3.5, where both fibrinogen molecule and the latex particles were positively charged, the maximum coverage varied between 0.9 mg m⁻² and 1.1 mg m⁻² for 10⁻² and 0.15 M NaCl, respectively. On the other hand, at pH = 9.7, the maximum coverage of fibrinogen was larger, varying between 1.8 mg m⁻² and 3.4 mg m⁻² for 10⁻² and 0.15 M NaCl, respectively. The experimental results were quantitatively interpreted by the numerical simulations⁻² and 3.4 mg m⁻² for 10⁻² and 0.15 M NaCl, respectively. The experimental results were quantitatively interpreted by the numerical simulations. Теоретично та експериментально дослiджено адсорбцiю фiбриногену на позитивно заряджених мiкросферах. Структура моношарiв i максимальне покриття були визначенi експериментально при pH = 3.5
 i 9.7 для концентрацiї NaCl в дiапазонi 10⁻³ − 0.15 M. Максимальне покриття фiбриногену на частинках латексу було визначене прецизiйно методом мiкроскопу атомної сили (AFM). Несподiвано, що при
 pH = 3.5, коли i фiбриноген i частинки латексу зарядженi позитивно, максимальне покриття змiнюється
 мiж 0.9 мг м⁻²
 i 1.1 мг м⁻²
 для 10⁻²
 i 0.15 M NaCl, вiдповiдно. При pH = 9.7 максимальне покриття фiбриногену є бiльшим i змiнюється вiд 1.8 мг м⁻²
 до 3.4 мг м⁻²
 для 10⁻²
 i 0.15 M NaCl, вiдповiдно. Експериментальнi результати пiдтвердженi чисельним моделюванням. en Інститут фізики конденсованих систем НАН України Condensed Matter Physics Modelling and measurements of fibrinogen adsorption on positively charged microspheres Моделювання та вимiрювання фiбриногенної адсорбцiї на додатньо заряджених мiкросферах Article published earlier |
| spellingShingle | Modelling and measurements of fibrinogen adsorption on positively charged microspheres Zeliszewska, P. Bratek-Skicki, A. Adamczyk, Z. Ciesla, M. |
| title | Modelling and measurements of fibrinogen adsorption on positively charged microspheres |
| title_alt | Моделювання та вимiрювання фiбриногенної адсорбцiї на додатньо заряджених мiкросферах |
| title_full | Modelling and measurements of fibrinogen adsorption on positively charged microspheres |
| title_fullStr | Modelling and measurements of fibrinogen adsorption on positively charged microspheres |
| title_full_unstemmed | Modelling and measurements of fibrinogen adsorption on positively charged microspheres |
| title_short | Modelling and measurements of fibrinogen adsorption on positively charged microspheres |
| title_sort | modelling and measurements of fibrinogen adsorption on positively charged microspheres |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/155785 |
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