Preparation of bifunctional silica polymer support for the synthesis of 3'-labeled oligonucleotides

• Published in: Біополімери і клітина
• Date: 2005
• Main Authors: Dubey, L.V., Dubey, I.Ya.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2005
• Subjects: Методи
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/155925
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Preparation of bifunctional silica polymer support for the synthesis of 3'-labeled oligonucleotides / L.V. Dubey, I.Ya. Dubey // Біополімери і клітина. — 2005. — Т. 21, № 4. — С. 365-369. — Бібліогр.: 27 назв. — англ.

Preparation of a silica polymer support with a linker arm containing protected amino and hydroxy groups is described. O-Benzotriazol-1-yl-N,N,N'‚N'-tetramethyluronium hexafluorophosphate (HBTU) in the presence of 1-hydroxybenzotriazole (HOBT) was used as an efficient activating reagent at...