Expression and secretion of human recombinant LIF by genetically modified mammalian cells

• Published in: Вiopolymers and Cell
• Date: 2011
• Main Authors: Rymar, S.Yu., Ruban, T.A., Irodov, D.M., Kordium, V.A.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2011
• Subjects: Molecular and Cell Biotechnologies
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/156336
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Expression and secretion of human recombinant LIF by genetically modified mammalian cells / S.Yu. Rymar, T.A. Ruban, D.M. Irodov, V.A. Kordium // Вiopolymers and Cell. — 2011. — Т. 27, № 1. — С. 53-58. — Бібліогр.: 29 назв. — англ., рос.

Aim. The aim of this work was to express the human LIF gene in mammalian cells and to study the secretion
 of recombinant LIF into culture medium. Methods. Recombinant LIF was detected by Western blot analysis
 and immunoprecipitation in culture medium of CHO-K1, L-M (TK–
 )...