Expression and secretion of human recombinant LIF by genetically modified mammalian cells

• Veröffentlicht in: Вiopolymers and Cell
• Datum: 2011
• Hauptverfasser: Rymar, S.Yu., Ruban, T.A., Irodov, D.M., Kordium, V.A.
• Format: Artikel
• Sprache: English
• Veröffentlicht: Інститут молекулярної біології і генетики НАН України 2011
• Schlagworte: Molecular and Cell Biotechnologies
• Online Zugang: https://nasplib.isofts.kiev.ua/handle/123456789/156336
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Zitieren: Expression and secretion of human recombinant LIF by genetically modified mammalian cells / S.Yu. Rymar, T.A. Ruban, D.M. Irodov, V.A. Kordium // Вiopolymers and Cell. — 2011. — Т. 27, № 1. — С. 53-58. — Бібліогр.: 29 назв. — англ., рос.

Aim. The aim of this work was to express the human LIF gene in mammalian cells and to study the secretion of recombinant LIF into culture medium. Methods. Recombinant LIF was detected by Western blot analysis and immunoprecipitation in culture medium of CHO-K1, L-M (TK– ) (ins+ ), 293Ò cells, tr...