Cloning, expression and purification of D-Tyr-tRNATyr-deacylase from Thermus thermophilus

• Published in: Вiopolymers and Cell
• Date: 2015
• Main Authors: Rybak, M.Yu., Kovalenko, O.P., Kryklyvyi, I.A., Tukalo, M.A.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2015
• Subjects: Structure and Function of Biopolymers
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/156342
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Cloning, expression and purification of D-Tyr-tRNATyr-deacylase from Thermus thermophilus / M.Yu. Rybak, O.P. Kovalenko, I.A. Kryklyvyi, M.A. Tukalo // Вiopolymers and Cell. — 2015. — Т. 31, № 3. — С. 179-186. — Бібліогр.: 24 назв. — англ.

D-Tyr-tRNATyr-deacylase (DTD) is a conservative enzyme, found in all domains of life, which ensures an additional checkpoint in the recycling of misaminoacylated D-Tyr-tRNATyr. DTD is capable of accelerating the hydrolysis of the ester linkage of D-Tyr-tRNATyr producing a free tRNA and D-tyrosine, t...