Клонирование и экспрессия гена SPAА Еrysipelothrix rhusiopathiae в Еscherichia coli
Цель. Клонировать ген SPAА – поверхностный антиген Er. rhusiopathiae – в плазмидный вектор и экспрессировать белок SpaА в E. coli. Методы. Расчет и синтез олигонуклеотидных праймеров, полиме -
 разная цепная реакция, клонирование, экспрессия рекомбинантных белков в E. coli, электрофорез в&am...
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| Published in: | Вiopolymers and Cell |
|---|---|
| Date: | 2011 |
| Main Authors: | Дерябин, О.Н., Дерябина, Е.Г., Гильчук, П.В. |
| Format: | Article |
| Language: | Russian |
| Published: |
Інститут молекулярної біології і генетики НАН України
2011
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| Subjects: | |
| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/156390 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Клонирование и экспрессия гена SPAА Еrysipelothrix rhusiopathiae в Еscherichia coli / О.Н. Дерябин, Е.Г. Дерябина, П.В. Гильчук // Вiopolymers and Cell. — 2011. — Т. 27, № 4. — С. 310-313. — Бібліогр.: 11 назв. — рос. |
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