A PCR-mediated method for discrimination of Klebsiella oxytoca between closely related bacteria in environmental and clinical specimens

• Published in: Біополімери і клітина
• Date: 2003
• Main Authors: Kovtunovych, G.L., Lytvynenko, T.L., Negrutska, V.V., Lar, O.V., Koltukova, N.V, Kozyrovska, N.O.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2003
• Subjects: Молекулярна та клітинна біотехнології
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/156684
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: A PCR-mediated method for discrimination of Klebsiella oxytoca between closely related bacteria in environmental and clinical specimens / G.L. Kovtunovych, T. L. Litvinenko, V.V. Negrutska, O.V. Lar, N.V. Koltukova, N.O. Kozyrovska // Вiopolymers and Cell. — 2003. — Т. 19, № 6. — С. 520-524. — Бібліогр.: 21 назв. — англ.

A specific detection method was developed to discriminate Klebsiella oxytoca between other species of the genus Klebsiella on the basis of PCR amplification of the unique DNA sequences within the polygalacturonase-encoding (pehX) gene. Four primers have been designed for performing PCRs gaining ampl...