Prolyl-tRNA synthetase from Thermus thermophilus is eukaryotic-like but aminoacylates prokaryotic tRNAPro

• Published in: Вiopolymers and Cell
• Date: 2012
• Main Authors: Yaremchuk, A.D., Boyarshin, K.S., Tukalo, M.A.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2012
• Subjects: Structure and Function of Biopolymers
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/156728
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Prolyl-tRNA synthetase from Thermus thermophilus is eukaryotic-like but aminoacylates prokaryotic tRNAPro / A.D. Yaremchuk, K.S. Boyarshin, M.A. Tukalo // Вiopolymers and Cell. — 2012. — Т. 28, № 6. — С. 434-440. — Бібліогр.: 22 назв. — англ.

Aim. Cloning, sequencing and expression of the gene encoding prolyl-tRNA synthetase, a class IIa enzyme, from the extreme thermophile T. thermophilus HB8 (ProRSTT). Methods. Search for the ProRSTT gene was performed by Southern blot hybridization with chromosomal DNA, the digoxigenin-labeled PCR fr...