Generation of monoclonal antibodies specific to ribosomal protein S6 kinase 1

• Veröffentlicht in: Вiopolymers and Cell
• Datum: 2012
• Hauptverfasser: Savinska, L.O., Klipa, O.M., Demchuk, N.O., Ovcharenko, G.V., Malanchuk, O.M., Tykhonkova, I.O., Palchevskyy, S.S., Filonenko, V.V.
• Format: Artikel
• Sprache: Englisch
• Veröffentlicht: Інститут молекулярної біології і генетики НАН України 2012
• Schlagworte: Structure and Function of Biopolymers
• Online Zugang: https://nasplib.isofts.kiev.ua/handle/123456789/156733
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Zitieren: Generation of monoclonal antibodies specific
 to ribosomal protein S6 kinase 1
 / L.O. Savinska, O.M. Klipa, N.O. Demchuk, G.V. Ovcharenko, O.M. Malanchuk, I.O. Tykhonkova, S.S. Palchevskyy, V.V. Filonenko // Вiopolymers and Cell. — 2012. — Т. 28, № 6. — С. 424-428. — Бібліогр.: 22 назв. — англ.

The aim of this study was to produce monoclonal antibodies directed to the N-terminal regulatory region of S6K1,
 which shows very low homology to S6K2. Methods. Hybridoma technology, ELISA, Western blot, Immunoprecipitation. Results. Three hybridoma clones (A1, A2 and A3) producing mAbs spe...