Екзогенні ДНК можуть впливати на регуляторні системи рослин, відповідальні за адаптацію до змін у довкіллі
Розроблено оригінальну технологію отримання рослин тютюну з комплексом селекційно цінних ознак (прискорений розвиток, висока продуктивність та стійкість до комплексного засолення грунтів) за допомогою препаратів екзогенної ДНК (е-ДНК), вивчено фізіолого-біохімічні особливості таких рослин та їхнє с...
Saved in:
| Published in: | Біополімери і клітина |
|---|---|
| Date: | 2006 |
| Main Authors: | , |
| Format: | Article |
| Language: | Ukrainian |
| Published: |
Інститут молекулярної біології і генетики НАН України
2006
|
| Subjects: | |
| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/156776 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Екзогенні ДНК можуть впливати на регуляторні системи рослин, відповідальні за адаптацію до змін у довкіллі / В.А. Кацан, А.І. Потопальський // Біополімери і клітина. — 2006. — Т. 22, № 4. — С. 307-316. — Бібліогр.: 61 назв. — укр., англ. |
Institution
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1859936416220315648 |
|---|---|
| author | Кацан, В.А. Потопальський, А.І. |
| author_facet | Кацан, В.А. Потопальський, А.І. |
| citation_txt | Екзогенні ДНК можуть впливати на регуляторні системи рослин, відповідальні за адаптацію до змін у довкіллі / В.А. Кацан, А.І. Потопальський // Біополімери і клітина. — 2006. — Т. 22, № 4. — С. 307-316. — Бібліогр.: 61 назв. — укр., англ. |
| collection | DSpace DC |
| container_title | Біополімери і клітина |
| description | Розроблено оригінальну технологію отримання рослин тютюну з комплексом селекційно цінних ознак (прискорений розвиток, висока продуктивність та стійкість до комплексного засолення грунтів) за допомогою препаратів екзогенної ДНК (е-ДНК), вивчено фізіолого-біохімічні особливості таких рослин та їхнє спадкування. Для індукування бажаних змін у жовтолистого сорту тютюну Крупнолистный 20 (КР20) використано ДНК солестійкої форми пасльону чорного Solanum nigrum L. і плазмід pCAMVNEO та рТі8628. Важлива її перевага — забезпечення ширшого спектра змін і більшого виходу змінених життєздатних рослин. На основі аналізу модифікацій, виявлених у тютюну та рослин модельної системи, використаних при розробці технології, запропоновано гіпотетичний механізм впливу е-ДНК на спадковість рослин.
Разработана оригинальная технология получения растений табака, обладающих комплексом селекционно ценных призна ков (ускоренное развитие, повышенная продуктивность, устойчивость к комплексному засолению почвы) при помощи препаратов экзогенных ДНК (э-ДНК), изучены физиолого-биохимические особенности таких растений и их наследование. Для индуцирования желаемых изменений у желтолистного сорта табака Крупнолистный 20 использована ДНК солеустойчивой формы паслена черного Solanum nigrum L., а также ДНК плазмид pCAMVNEO и pTi8628. Важное преимущество разработанной технологии — обеспечение более широкого спектра изменений и более значительный выход измененных жизнеспособных растений. На основе анализа модификаций, обнаруженных у табака и растений модельной системы, использованных при разработке технологии, предложен гипотетический механизм влияния э-ДНК на наследтвенные признаки растений.
The original technology of obtaining the tobacco plants possessing the complex of selectively useful features (accelerated development, high productivity, and resistance to complex salinization) has been elaborated, and inheritance of the physiological and biochemical peculiarities of such plants have been investigated. To get the important and selective changes of yellow-leaved tobacco Krupnolistny 20 (KR 20, Large-leaved 20) cultivar, the native and alkylated by thiophosphamide DNA of salt-tolerant nightshade (Solanum nigrum L.) and DNA of pCAMVNEO and pTi8628 plasmids have been used. The valuable advantage of such technology is the provision of a wider range of changes and larger output of changed viable plants. The changes obtained by DNA action on tobacco and other plants exploited in technology elaboration have been analysed, and possible mechanism of exogenic DNAs influence on plant heredity has been proposed.
|
| first_indexed | 2025-12-07T16:09:35Z |
| format | Article |
| fulltext |
Exogenic DNAs May Influence Plant Adaptation
Reactions to Changed Environment
V.A. Katsan, A.I. Potopalsky
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine
150, Zabolotny Str., Kyiv, 03143, Ukraine
e-mail: potopalsky@imbg.org.ua
The original technology of obtaining the tobacco plants possessing the complex of selectively useful features
(accelerated development, high productivity, and resistance to complex salinization) has been elaborated, and
inheritance of the physiologic and biochemic peculiarities of such plants has been investigated. To get the
important and selective changes of yellow-leaved tobacco Krupnolistny 20 (KR 20, Large-leaved 20) cultivar, the
native and alkylated by thiophosphamide DNA of salt-tolerant nightshade (Solanum nigrum L.) and DNA of
pCAMVNEO and pTi8628 plasmids have been used. Its useful advantage is the provision of a wider change range
and larger output of changed viable plants. The changes obtained by DNA action on tobacco and other plants
exploited in technology elaboration have been analysed, and possible mechanism of plant heredity change by
exogenic DNAs has been proposed.
Keywords: plants from solanaceous family, exogenous DNA, alkylated by thiophosphamide DNA, chlorophylls,
carotene, xanthophylls, violaxanthine, luteine, salt resistance
Introduction The problem of breeding plant cultivars,
capable of not only surviving in changed environment,
but also of having proper productivity level, is as actual
as never before. The problem of salinization is of
special importance as in the world scale the area of
salinized soil amounts to 900 million hectares and is
constantly growing due to the usage of intensive
agrarian technologies, artificial watering, as well as the
introduction of soils, which are already salinized due to
natural conditions, into usage [1, 2]. The salinization of
agricultural fields is a problem of more than 100
countries of the world, including the states that were a
part of the Soviet Union, where, according to the data of
the previous century end, it was about 10% on average,
while in the countries of Middle Asia it reached 50-80%
[3]. Another important problem is climate changes and
environment pollution by xenobiotics, which have both
toxic and mutagenic influence on plants.
To obtain new forms of plants with a complex of
selectively useful features, it is still more important to
search the ways of variability expanding and these
features transfer from wild plants.
The resistance to unfavourable conditions of
environment, as well as quantitative features,
determining the productivity and crop structure of
plants, are polygenic. The genes, controlling the
mentioned features, are disposed in different linkage
groups,, therefore, there are difficulties in their transfer
by the methods of gene engineering. For the first time
the mutagenic action of exogenous DNAs was shown
67 years ago in the studies of Tarnavsky [4, 5] and
Gershenson [6]. Gershenson et al. established the
general features of action of natural and synthetic
307
ISSN 0233-7657. Biopolymers and cell. 2006. Vol. 22. ISS 4. Translated from Ukrainia.
ãV.A. KATSAN, A.I. POTOPALSKY, 2006
GENOME AND ITS REGULATION
polynucleotides on living organisms [7, 8], and in the
70-80s of the previous century the possibility of
obtaining selectively useful features of plants using
DNA preparations was proved [9-12]. The mechanisms
of exogenous DNAs (e-DNA) influence on heredity is a
subject for discussions even nowadays, therefore, the
investigation in this direction is not intensive enough.
As a result of investigations, which were conducted
for more than thirty years, the methodology of
changing plant heredity information using e-DNA
preparations was elaborated in our laboratory, the
important features of it are DNA selection as a donor of
desired features or a mutagene (depending on
taxonomic affinity of both the donor and the recipient),
obtaining DNA preparations of the donor and their
alkylation using tri-functioning alkylating agent
thiophosphamide DNA (e-DNT), and the influence of
mentioned preparations on germinating seeds of the
recipient. The usage of these approaches allowed
obtaining more than 40 new forms and cultivars of
plants with desired features, among which there are the
ones, which passed the State approbation of cultivars,
are registered in Ukraine and recommended for
practical application [9]. DNA concentration, the stage
of seed germination, and the conditions of its
infiltration with DNA solutions were selected
individually for each plant cultivar in the experimental
way.
The goal of this work is to elaborate the technology
of obtaining new forms of tobacco plants with the
complex of selectively useful features, among which
are salt resistance, early maturation, high productivity,
which could be used for breeding of modern cultivars,
as well as to analyze e-DNA induced changes in plants.
Materials and Methods The tobacco cultivar,
selected as a donor. The investigation was performed
on the pure line of tobacco Krupnolistny 20 (KR 20)
cultivar (the author O.P. Grebyonkin, NPO Tabak,
Russia), the seeds of which were kindly given to us by
B.O. Levenko (Gryshko National Botanical Gardens,
NAS of Ukraine). KR 20 cultivar is distinguished by
early apoptosis of industrial layer leaves. The plants
acquire characteristic phenotype with light yellow
colouring of middle layers leaves and green colouring
of only plant tops on the primary stages of floral shoot
growing. This phenotype is conditioned by the
dominant mutation White, obtained by
Kosmodemyansky using the method of chemical
mutagenesis in 1965 [13]. Mutants, similar in
phenotype, occur spontaneously as well, they are
known for practically all agroecological tobacco
groups and can be a consequence of mutations of both
dominant and recessive genes [14]. The selectionists’
attention was attracted to yellow-leaved mutations due
to the fact that one of important indicators of raw
material quality in tobacco production is the colour of
leaves after their breaking off, withering, and drying –
the leaves, getting even golden-brown colouring are
considered of high quality, and it is yellow-leaved
tobacco cultivars that give the best output of highly
qualitative raw material by colour feature [15].
However, all the mutations, known nowadays, which
condition early apoptosis of industrial layer leaves of
tobacco, are undoubtedly harmful, as they decrease
plant viability and the content of economically useful
substances in leaves which results in general quality
loss of raw material.
The basis of the study on interconnection of
biochemical indicators with the raw material quality for
tobacco production was laid by A.A. Shmuk: first of all,
besides absence of chlorophylls, the raw material of
high quality is characterized by high content of
carbohydrates, and optimal correlation among
carbohydrates and proteins (Shmuk’s number) [16].
The correlation of chlorophylls content to biochemical
features of raw material quality in Krupnolistny
tobacco cultivars was studied before [17].
The plants, used in a model system. New
approaches were used while elaborating the technology
of changing tobacco heredity features, therefore, the
action of exogenous DNA was studied on the model
system using KR 20 tobacco as well as wild plants from
solanaceous family namely, stramonium Datura
stramonium L., and cultivated plants of other families –
sorghum Horizont cultivar, millet Chervona Vatra
cultivar, corn Delikatesna cultivar, poppy somniferous
Novynka cultivar. Pure lines of these plants were
obtained in our laboratory..
The plant – donor of selectively useful features.
Salt-tolerant form of nightshade – Solanum nigrum L.
was used as a donor. Salt-tolerant form of nightshade is
a line of the plant, taken from natural population, and
308
KATSAN V.A., POTOPALSKY A.I.
adapted to growing in the conditions of complex
salinization (Rybakivka village, Ochakivsky district,
Mykolayivsky region).
Obtaining DNA preparations. DNA extraction of
nightshade and alkylation of obtained preparations by
thiophosphamide were performed according to the
methods, elaborated in our laboratory [18, 19]. Purified
DNA pCAMVNEO, containing the resistance gene to
kanamycin from the bacterial transposon Tn5, was
kindly given to us by O.P. Smirnov (Vavilov Institute
of General Genetics, ,Russian Academy of Sciences,
Moscow). DNA pTi8628 was extracted from
Agrobacterium tumefaciens strain, infecting the
cultures of plants [20].
The methods of statistical mathematics. To process
the obtained results of the study we used the methods of
estimating the average probability and the probability
of differences between the experiment variants and the
control by Student’s criterion, selection of samples
with a large variation range in classes as well as one
factor scheme of variance analysis [21, 22].
Results and Discussion The optimal concentration
of DNA preparations in the solutions for tobacco seeds
infiltration was chosen in the experimental way,
considering the data, obtained in our laboratory on
tomatoes and other plants before, it amounted to
500µg/ml. DNA was introduced into the germinating
seeds by the infiltration method. New approaches, used
by us for technology elaboration, are as follows:
-to extend variability range of investigated features,
the DNAs of the donor plant were used in the complex
with the DNA of plasmids, which are the source of
known mobile genetic elements (pCAMVNEO,
pTi8628);
-the duration of DNA preparations action was
changed;
-the conditions of performing the germinating seeds
infiltration by DNA solutions were changed.
The results of the study showed expressed genotype
specificity of the influence of the DNA, used by us, on
the selected plants, especially concerning genotoxic
action, output of viable fertile plants and the ones,
which had desired features as early as in the first
generation (Table). The DNA of plasmids manifested
the highest toxicity on the model system plants. The
DNA of the nightshade in the chosen concentration was
genotoxic for tobacco, therefore, its concentration in
the complex preparation was decreased 3-fold. The
differences between the action of the alkylated and
native DNA of nightshade also depended on the
recipient genotype. The alkylation of nightshade DNA
with thiophosphamide allowed not only removing its
genotoxic effect on millet and tobacco at its usage in a
high concentration (500µg/ml), but also obtaining
viable fertile plants, while alkylated DNA of
nightshade turned out genotoxic for poppy. DNA
pTi8628 was genotoxic for both tobacco and sorghum;
DNA pTi8628 alkylation allowed obtaining fertile
tobacco plants, but the germination of nightshade seeds
decreased. The alkylation with thiophosphamide DNA
pCAMVNEO did not allow removing its genotoxic
influence on all plants cultivars, studied by us, except
tobacco: using e-DNT pCAMVNEO we managed to
obtain one tobacco plant (#73) which turned out to be
fertile. In general, using the approaches, elaborated by
us, fertile plants were obtained for all the investigated
cultivars, except corn. In the majority of experiment
variants the desired changes in the presence of viable
plants were observed on the tobacco cultivar KR 20.
The technology, elaborated by us, allowed
obtaining tobacco plants with changed phenotype
concerning leaves colouring as well as with the
preservation of chlorophylls in the leaves of industrial
layers before the development completion, which also
were distinguished by fast development and early terms
of blooming comparatively to the control, they had
large leaves and these features were inherited [25-27].
Salt resistance heredity was shown for plants from
the variants where DNA or DNT of salt tolerant form of
nightshade were used (6.3 ± 0.14 – 7.7 ± 0.11 and 9.8 ±
0.10 – 10.4 ± 0.12% of viable sprouts respectively,
obtained in the medium with 20g/l sea salt from the
plant seeds of the first generation). Likewise tomatoes
[3, 9], selectively useful changes occurred as early as
the first generation.
Obtaining of salt tolerant tobacco forms [25, 26]
may prove genetic transformation of tobacco using the
DNA of salt tolerant nightshade. Global changes in
metabolism are known to take place in plants at salt
stress which is caused by expression changes of about
1500 genes [27]. The nature of salt resistance in plants
is controlled by a great number of genes and may be
309
DNAS MAY INFLUENCE PLANT ADAPTATION REACTIONS
conditioned by phenomena which are provided by the
mechanisms of control of ions transport and antiport
through the plasmatic membrane, the transport of ions
surplus from the cytoplasm into vacuoles and by the
biosynthesis of substances that have protection features
at abiotic stresses (proline, betaines, trehalose,
myoinositol, polyamines, etc) as well as by the
activation of systems, neutralizing reactive oxygen
forms at the participation of glutathione and by the
expression increase of nucleic acids processing genes,
DNA-helicase and biosynthesis of PR-proteins, in
particular [28-36]. Salt resistance in plants is practically
310
KATSAN V.A., POTOPALSKY A.I.
The influence of e-DNA and e-DNT preparations on the germination and appearance of the most characteristic phenotype changes in T
1
plants
DNA
preparation
DNA
concentration,
µg/ml
Krupnolistny tobacco 20 Datura str amonium L. Millet Chervona Vatra
Germina-tion,
%
Phenotype
changes, %
Germin-ation,
%
Phenotype
changes, %
Germina-tion,
%
Phenotype
changes, %
Nightshade DNA 500 0 - 11.7* A, 46.7* 0 -
Nightshade DNT 500 46.7** Gr, 100.0; EB, 94.9* 10.3** A, 50.0** 0.7** D, 50.0**
pTi8628 DNA 500 0 - 15.0* A, 48.9* 6.0* D, 100.0
pTi8628 DNT 500 4.0* Gr, 100.0; EB 75.0* 2.7** A, 49.9* 0 -
pCAMVNEO DNA 500 2.7* Gr, 100.0; EB 75.0* 0 - 0 -
pCAMVNEO DNT 500 0.3 (one plant) Gr, EB 0 - 0 -
DNA1 complex 500 (total) 31.7* Gr, 100.0; EB, 89.1* 0 - 1.0* D, 100.0
DNT2 complex 500(of each DNT) 0 - 4.3* A, 56.0* 0 -
Control H
2
O 97.3** 197 98.5** - 97.8** -
DNA
preparation
DNA
concentration,
µg/ml
Sorghum Horizont Corn Delikatesna Somniferous poppy Novynka
Germina-tion,
%
Phenotype
changes, %
Germina-tion,
%
Phenotype
changes, %
Germina-tion,
%
Phenotype
changes, %
Nightshade DNA 500 2.7* LH, 100.0 0 - 30.0*
L (DF), 85.3*; Chl,
25.0*; SL, 50.0*
Nightshade DNT 500 2.0 LH, 100.0 0 - 0 -
pTi8628 DNA 500 0 - 46.7*
ALS, 46.2*; SI,
30.8*; S, 100.0
45.0
L (DF), 76.3*; Chl,
15.4*; SL, 21.9*
pTi8628 DNT 500 0 - 0 - 0 -
pCAMVNEO DNA 500 0 - 0 - 0 -
pCAMVNEO DNT 500 0 - 0 - 0 -
DNA1 complex 500 (total) 1.7* LH, 100.0 6.7* L, 100.0 0 -
DNT2 complex 500(of each DNT) 0 - 0 - 0 -
Control H
2
O 96.9** - 96.7** - 98.3** -
Note. 1DNA complex of nightshade and pCAMVNEO and pTi8628 plasmids, each DNA concentration is the third of 500µg/ml; 2DNT complex of the same
origin, each DNT concentration – 500µg/ml. The variability degree of the given maximum values of the investigated parameters: *p>0.95; **p>0.99 at
n=5. Phenotypes deciphering: A – anthocyane colouring of flowers and plants (stramonium): L – lethality on early stages of development; DF – dwarfish
plants with anthocyane colouring (poppy); Chl – light (light green) colour of leaves; SL – small leaves; D– dwarfish plants; LH – low height plants; Gr –
“green” development type (chlorophylls preservation in the leaves of industrial layers to the development completion, tobacco); EB – early blossoming
phenotype (tobacco); ALS - anthocyane colouring of leaves and stems (corn); SI – “spike-like male inflorescence” (corn); S – sterility (connected with
heterochronous development anthers and ears of corn).
a quantitative feature as it can be increased due to the
reinforcement of one of these metabolism pathways by
employing genetic construction containing 1-2 genes
[28-35]. Several mechanisms can participate in the
acquisition of salt resistance by plants, therefore, using
e-DNA, it is much simpler and more reliable to obtain
salt tolerant plants which was proved by our
investigations on tobacco and by obtaining a salt
tolerant tomato cultivar [3, 9].
The characteristic feature of the influence of
e-DNAs, used according to the elaborated technology,
is the induction of a large amount of changes in the first
generation plants. Practically all the obtained tobacco
plants turned out to be changed – in height,
development terms, form and colouring of leaves. The
most characteristic feature was the recovery of the wild
green phenotype by plants . The inheritance of this
feature was shown in the lines of plants, selected from
different variants [23-26]. Also we revealed e-DNA
capability of inducing both quantitative and qualitative
hereditary changes in the accumulation of
photosynthetic pigments, which were analysed by us in
detail [23, 24].
Among modifications, obtained from plants using
e-DNA, there are some which may serve as a proof of
e-DNA influence on genetic systems, responsible for
the adaptation to changed environment. First of all, this
is the inheritance of salt resistance and some changes in
photosynthetic pigments accumulation, the changes in
chlorophylls a and b ratio, obtained from tobacco using
DNA of nightshade, in particular [24-26]. The reaction
of chlorophyll a conversion into chlorophyll b is known
to be catalysed by chlorophyllid a oxygenase, it
regulates the size of the main light-harvesting complex
(LHC II) and is underlying the plants adaptation to the
changes of lightening conditions [37]. The increase of
violaxanthine cycle pigments amount [24-26] can also
be important for adaptation, as the compounds, which
are generated by violaxanthine cycle, regulate safe
dissipation of the excess of absorbed energy [38].
Besides, violaxanthine is a substrate for the
biosynthesis of the abscisic acid, which is very
important for the stress responses in plants [39]. In its
turn, the general increase of xanthophylls and carotene
content [24] may be important for adaptation processes,
considering a significant biological function of these
pigments as scavengers of free radicals, that are formed
out of oxygen and chlorophylls and may become a
reason of damaging photosynthetic membranes. The
increased portion of chlorophyll a, which is a part of the
reaction centres, and lutein [24, 26] which is a
component of pigment complexes, surrounding the
reaction centre, may indicate the increase of
photosystems amount. The increased amount of
chlorophylls in tobacco testifies to the resistance to
diseases: tobacco mosaic, peronosporosis, and
phytophthorosis [40]. Quantitative and qualitative
changes in the photosynthetic apparatus are likely to be
directed towards generating compositions and energy,
necessary for plant adaptation to changed environment,
as photosynthesis is the most sensitive function of
plants, which reacts to stressors first and foremost,
therefore the composition and qualitative correlations
of photosynthesis products change considerably
depending on the organism condition [41].
Both the appearance of anthocyanin pigments in the
plant top and the change of flowers colouring from
usual white to violet colour may have protective
meaning, which was revealed in the investigation of
stramonium (D. stramonium), and this feature was also
inherited. The change of stramonium crown colouring
also reminds phenomena, conditioned by the
transposon transfer [42].
Another group of features, caused by e-DNA in T
1
tobacco plants, is non specific reactions of resistance to
kanamycin and to spontaneous infecting by viruses,
which are present in the environment [43]. It may
testify to the induction of changes in metabolism,
connected with the adaptation to some stress agents.
The proof of e-DNA influence on regulatory
systems, responsible for the adaptation to changed
environment, may be in morphological changes,
obtained in T
1
plants under the influence of e-DNA:
reduced stems of Chervona Vatra millet (like mountain
plants), smaller leaves and narrowed leaf plate in
tobacco and poppy (a xenomorphic feature), the
decrease of height and the appearance of brachytic
forms among the plants of all the investigated cultivars
(Table).
Hereditary changes in chlorophylls accumulation,
revealed in tobacco, as well as hereditary changes of
plants habitus (height decrease, inhibition of apical
311
DNAS MAY INFLUENCE PLANT ADAPTATION REACTIONS
domination with the formation of a significant amount
of side shoots) resemble the complex of hereditary
changes, obtained in tobacco mutants with the
increased expression of phytochrome A gene,
modulating the level of gibberellins in the vascular
tissues [44]. Tobacco mutants with decreased
expression of phytochrome B gene are characterised by
a complex of other changes – apical domination and
chlorophylls content like mutants of chlorine [45].
Functioning chloroplasts are necessary for the
establishment of system acquired resistance in plants.
The interaction of signal transduction pathways,
induced by light, with signal pathways, initiated by
phytopathogens and mediated by salicylic acid, takes
place in this case [46, 47], while the resistance to
pathogens may be caused by changing phytochrome
genes expression [46].
The analysis of our investigation results as well as
literature data concerning the molecular nature of
phenomena and processes, which are influenced by
e-DNA, may become the foundation of the hypothesis
concerning one of important ways of e-DNA influence
on plants – it is implemented at the action on sprouting
seeds and it causes the changes of expression and genes
mutations, responsible for the recognition of signals
about changed environment and the direction of
responses to these changes. The key genes of signal
systems are surely the most open and available ones,
hence they change in the first place; they are also cross
points of signal ways interacting, initiated by
phytochromes, nutrients and agents of abiotic and
biotic stresses [48-50], which conditions pleiotropic
effect of these mutations.
Instability of the chlorophylls content in tobacco
(the appearance of plants phenotypes in T
1
which were
1.4-3.7 times different in chlorophylls content, and
splitting of selected plants in T
2
generation into forms
that were two times and more different in chlorophylls
content [23, 24] as well as the appearance of flowers
with white and mosaic colouring in T
1
(Figure)) may
evidence to e-DNA influence on systems, regulating
transposon transfer. The phenomenon of form-building
process activation in plants with the participation of
mobile genetic elements under the influence of changed
environment was discovered by McClintock [51]. The
universality of the transposon transfer activation of
drosophila at the influence of different stress agents
(heat-shock, chemical mutagenes, and e-DNA of viral
origin) was shown by Georgiev [52].
A considerable contribution into the study of
systems functioning of genome regulation using mobile
genetic elements was made by Ratner (the study of
block-module principle of genome organization and
mobile systems of genetic information regulation)
[53-55]. The study of Moscow scientists is devoted to
the significance of mobile genetic elements in the
adaptive changes occurrence [56, 57]. The similarity of
genes changes, caused by e-DNA in drosophila, with
the effects, conditioned by the “controlling elements
action” (exceptional locus-specificity, prolonged
action, and instability in generations) was remarked by
Gershenson who supposed that e-DNA fragments can
act as transposons, destabilising the recipient genes [58,
59], later Alexandrov and Gershenson suggested a
hypothesis concerning the e-DNA influence
mechanism due to the activation of transposons transfer
in the recipient [60]. The participation of transposons in
the expression of hereditary changes in drosophila due
to e-DNA action was also proven by experiments [8].
In our opinion, the spectrum of changes, induced in
drosophila by e-DNA, is worth considering as these are
mainly mutations connected with the change of sizes,
forms, and nervation [59], which can have adaptive
significance for insects. Adaptive changes in plants
were revealed in our investigations as well as by many
other scientists [9-12]. Thus, one of the action
mechanisms of exogenous DNA on hereditary features
of the recipient, which is very important for
selectionists, can be the activation of regulation
systems, which are responsible for the adaptation to
changed environment that may find its implementation
not only in the expression changes of the most
important genes but also in their hereditary
modifications.
At the e-DNA influence on plants, used in our
experiments, we observed the appearance of features,
which are characteristic of plants of related taxons that
may be the demonstration of parallel variability
according to Vavilov [61]. These changes may include
the appearance of violet flowers of usual stramonium
(the hereditary features, which are similar to that of
Indian stramonium), the spike-like form of male
312
KATSAN V.A., POTOPALSKY A.I.
313
DNAS MAY INFLUENCE PLANT ADAPTATION REACTIONS
Plants with changed colouring of leaves in T
1
generation: a – white flowers of plant #60 of the 1st generation in experiment #1 variant, where we used DNA
complex of nightshade and pTi8628 and pCAMVNEO; b – mosaic petal colouring of plant #73 from experiment #3 variant (DNT pCAMVNEO)
inflorescence of corn, the formation of a number of side
shoots of tobacco, the decrease of cycle duration of its
development and the change of flowers colour from
pink to white (the features of nightshade). In our
opinion, it is Vavilov’s law that is one of the most
important manifestations of formative processes in
plants, conditioned by fundamental features of the
systems of preservation and regulation of genetic
information, which were specified by Ratner [53-55].
Conclusions
1. The original technology of obtaining tobacco
plants with desired hereditary features namely,
resistance to complex salinization, early
maturation, increased productivity, using e-DNA
was elaborated. The result of its usage was the
obtaining of tobacco lines which may become the
basis for the breeding of modern highly productive
cultivars, resistant to complex salinization.
2. On the basis of the analysis of changes, obtained
in tobacco using e-DNA, as well as changes which were
observed at the influence of e-DNA on plants and
drosophila by other researchers, the theoretical
mechanism of e-DNA influence on plant heredity was
suggested. It can be used in both biotechnology and
selection.
Â. À. Êà öàí, À. È. Ïî òî ïà ëüñêèé
Ýêçî ãåí íûå ÄÍÊ ìî ãóò âëè ÿòü íà ðå ãó ëÿ òîð íûå ñèñ òå ìû ðàñ òå íèé, îò -
âå ÷à þ ùèå çà àäàï òà öèþ ê èç ìå íå íè ÿì â îêðó æà þ ùåé ñðåäå
Ðå çþ ìå
Ðàç ðà áî òà íà îðè ãè íàëü íàÿ òåõ íî ëî ãèÿ ïî ëó ÷å íèÿ ðàñ òå íèé òà áà êà,
îá ëà äà þ ùèõ êîì ïëåê ñîì ñå ëåê öè îí íî öåí íûõ ïðè çíà êîâ (óñêî ðåí íîå
ðàç âè òèå, ïî âû øåí íàÿ ïðî äóê òèâ íîñòü, óñòîé ÷è âîñòü ê êîì ïëåê ñíî -
ìó çà ñî ëå íèþ ïî ÷âû) ïðè ïî ìî ùè ïðå ïà ðà òîâ ýê çî ãåí íûõ ÄÍÊ
(ý-ÄÍÊ), èç ó÷å íû ôè çè î ëî ãî-áè î õè ìè ÷åñ êèå îñî áåí íîñ òè òà êèõ ðàñ -
òå íèé è èõ íà ñëå äî âà íèå. Äëÿ èí äó öè ðî âà íèÿ æå ëà å ìûõ èç ìå íå íèé ó
æåë òî ëèñ òíî ãî ñî ðòà òà áà êà Êðóï íî ëèñ òíûé 20 èñ ïîëü çî âà íà ÄÍÊ
ñî ëå óñ òîé ÷è âîé ôîð ìû ïàñ ëå íà ÷åð íî ãî Solanum nigrum L., à òàê æå
ÄÍÊ ïëàç ìèä pCAMVNEO è pTi8628. Âàæ íîå ïðå è ìó ùåñ òâî ðàç ðà áî -
òàí íîé òåõ íî ëî ãèè ? îá åñ ïå ÷å íèå áî ëåå øè ðî êî ãî ñïåê òðà èç ìå íå íèé
è áîëåå çíà÷èòåëüíûé âûõîä èçìåíåííûõ æèçíåñïîñîáíûõ ðàñòåíèé.
Íà îñíî âå àíà ëè çà ìî äè ôè êà öèé, îá íà ðó æåí íûõ ó òà áà êà è ðàñ òå íèé
ìî äåëü íîé ñèñ òå ìû, èñ ïîëü çî âàí íûõ ïðè ðàç ðà áîò êå òåõ íî ëî ãèè,
ïðåä ëî æåí ãè ïî òå òè ÷åñ êèé ìå õà íèçì âëè ÿ íèÿ ý-ÄÍÊ íà
íàñëåäòâåííûå ïðèçíàêè ðàñòåíèé.
Êëþ ÷å âûå ñëî âà: ïàñ ëå íî âûå, ýê çî ãåí íûå ÄÍÊ, àë êè ëè ðî âàí íàÿ òè -
î ôîñ ôà ìè äîì ÄÍÊ, õëî ðî ôèë ëû, êà ðî òè íû, êñàí òî ôèë ëû, âè î ëàê ñàí -
òèí, ëþ òå èí, ñî ëå óñ òîé ÷è âîñòü.
REFERENCES:
1. Flowers T. G. Improving crop salt tolerance // J. Exp.
Bot.—2004.—55.—P. 307—319.
2. Schoups G., Hopmans J. W., Young C. A., Vrugt J. A., Wallender W.
W., Tanji K. K. Sustainability of irridated agriculture in the San
Joaquin Valley, California // Proc. Nat. Acad. Sci.
USA.—2005.—102.—P. 15352—15356.
3. Ïî òî ïà ëüñêèé À. È., Êà öàí Â. À., Þðêå âè÷ Ë. Í., Êî âà ëåâ Â. À.
Òî ìà òû ñî ðòà Óêðà èí ñêèé ñî ëå óñ òîé ÷è âûé è ïåð ñïåê òèâ íûå ëè -
íèè, ïî ëó ÷åí íûå íà èõ îñíî âå // Îâî ùå âî äñòâî è áàõ ÷å âî -
äñòâî.—2005.—51.—Ñ. 168—180.
4. Òàð íàâ ñêèé Í. Ä. Ê âîï ðî ñó î ðîëè íóê ëå è íî âîé êèñ ëî òû ïðè
êîíú þ ãà öèè õðî ìî ñîì // Äîêë. ÀÍ ÑÑÑÐ.—1938.—20, ¹ 9.—Ñ.
721—724.
5. Òàð íà âñüêèé Ì. Ä. Äî ïè òàí íÿ ïðî ðîëü íóê ëå¿ íî âî¿ êèñ ëî òè ïðè
âèê ëè êàííi íà ïðàâ ëå íèõ ìó òàöié // Äîï. ÀÍ ÓPÑÐ.—1939.—¹
1.—Ñ. 47—49.
6. Ãåð øåí çîí Ñ. Ì. Âû çû âà íèå íà ïðàâ ëåí íûõ ìó òà öèé ó Drosophila
melanogaster // Äîêë. ÀÍ ÑÑÑÐ.—1939.—25, ¹ 3.—Ñ.
224—227.
7. Ãåð øåí çîí Ñ. Ì., Àëåêñàíäðîâ Þ. Ì., Ìà ëþ òà Ñ. Ñ. Ìó òà ãåí íîå
äå éñòâèå ÄÍÊ è âè ðó ñîâ ó äðî çî ôè ëû.—Êèåâ: Íàóê. äóì êà,
1975.—159 ñ.
8. Ãåð øåí çîí Ñ. Ì. Èçáè ðà òåëü íîñòü ìó òà ãåí íî ãî äå éñòâèÿ ÄÍÊ è
äðó ãèõ ïî ëè íóê ëå î òè äîâ // Æóðí. îáù. áè î ëî ãèè.—1996.—57,
¹ 6.—P. 661—682.
9. Êà öàí Â. À., Ïî òî ï àëüñüêèé À. I., Þðêå âè÷ Ë. Í. Îòðè ìàí íÿ ðîñ -
ëèí ç ãîñ ïî äà ðñüêî öiííè ìè îçíà êà ìè çà äî ïî ìî ãîþ åê çî ãåí íèõ
ÄÍÊ // Ìà òåð ià ëè ìiæíàð. ôî ðó ìó «Îñíî âè ìî ëå êó ëÿð íî-ãå íå -
òè÷ íî ãî îçäî ðîâ ëåí íÿ ëþ äè íè i äîâêiëëÿ» (Êè¿â, 31 òðàâ íÿ—1
÷åð âíÿ 2005 ð.).—Êè¿â, 2005.—Ñ. 84—87.
10. Ñè âî ëàï Þ. Ì., Îáðàç öîâ Ñ. È. Âîç ìîæ íîñòü ãå íå òè ÷åñ êîé
òðàíñ ôîð ìà öèè ó âû ñøèõ ðàñ òå íèé // Ðåñ ïóáë. ìåæ âåä. ñá. «Ìî -
ëå êó ëÿð íàÿ áè î ëî ãèÿ» (Êèåâ).—1980.—Âûï. 26.—Ñ. 3—8.
11. Êàð òåëü Í. À. Ýôôåê òû ýê çî ãåí íîé ÄÍÊ ó âû ñøèõ ðàñ òå -
íèé.—Ìèíñê: Íà ó êà è òåõ íè êà, 1981.—143 ñ.
12. Ëàð ÷åí êî Å. À., Ìîð ãóí Â. Â. Ýêñïå ðè ìåí òàëü íàÿ èç ìåí ÷è âîñòü
êó êó ðó çû.—Êèåâ: Íàóê. äóì êà, 1993.—173 ñ.
13. Êîñ ìî äåìü ÿí ñêèé Â. Í., Ðó áàí Ý. Â., Èâà íî âà Ò. Ç. Äî ìè íàí òíàÿ
ìó òà öèÿ White, èí äó öè ðî âàí íàÿ N-íè òðî çî ìî ÷å âè íîé ó òà áà êà //
Ïðàê òè êà õè ìè ÷åñ êî ãî ìó òà ãå íå çà.—Ì.: Íà ó êà, 1971.—Ñ.
179—183.
14. Áó ÷èí ñêèé À. Ô. Îñî áåí íîñ òè æåë òî ëèñ òíûõ ôîðì Nicotiana
tabacum L. // Ñá. ðà áîò ïî ñå ëåê öèè, ãå íå òè êå è ñå ìå íî âå äå íèþ
òà áà êà è ìà õîð êè (ÂÍÈÈÒèÌ).—Êðàñ íî äàð, 1936.—Âûï.
132.—Ñ. 37—54.
15. Èâà íî âà Ò. Ç. Ïî ïó ëÿ òèâ íîñòü ñî ðòîâ òà áà êà ïî öâå òó è êà ÷åñ òâó
// Òà áàê.—1967.—¹ 4.—Ñ. 53—54.
16. Øìóê À. À. Õè ìèÿ è òåõ íî ëî ãèÿ òà áà êà.—Ì.: Ïè ùåï ðî ìèç äàò,
1953.—T. 3.—776 ñ.
17. Áà ðà íî âà Å. Ã. Ãå íå òè ÷åñ êèå îñî áåí íîñ òè íà ñëå äî âà íèÿ ïðè çíà -
êîâ õè ìè ÷åñ êî ãî ñî ñòà âà ñî ðòîâ òà áà êà è èñ ïîëü çî âà íèå èõ â ñå -
ëåê öèè: Àâòîðåô. äèñ. ... êàíä. áèîë. íàóê / ÍÈÈ çåì ëå äå ëèÿ ÀÍ
Àðìåíèè.—Ý÷ìè àä çèí, 1990.—25ñ.
18. À. ñ. ÑÑÑÐ ¹ 1170871 Ò, ÌÊÈ C 12 N 15/00, C 07 H 21/00. Ñïî -
ñîá ïî ëó ÷å íèÿ äåç îêñè ðè áî íóê ëå è íî âîé êèñ ëî òû èç ðàñ òè òåëü -
íî ãî ñûðüÿ / Ç. Þ. Òêà ÷óê, À. È. Ïî òî ïà ëüñêèé // Âû äà íî
01.04.85.
19. Âî ëî ùóê Ò. Ï., Ïàö êîâ ñêèé Þ. Â., Ïî òî ïà ëüñêèé À. È.
Àëêèëèðîâàíèå êîì ïî íåí òîâ íóê ëå è íî âûõ êèñ ëîò ýòè ëå íè ìè -
íîì è åãî ïðî èç âîä íû ìè. 4. Àëêèëèðîâàíèå ãî ìî íóê ëå î òè äîâ è
ÄÍÊ // Áè î îðã. õè ìèÿ.—1999.—25, ¹ 6.—Ñ.464—473.
20. Ìà íè à òèñ Ò., Ôðè÷ Ý., Ñýì áðóê Äæ. Ìî ëå êó ëÿð íîå êëî íè ðî âà -
íèå.—Ì.: Ìèð, 1984.—479 ñ.
21. Êî êó íèí Â. À. Ñòà òèñ òè ÷åñ êàÿ îá ðà áîò êà äàí íûõ ïðè ìà ëîì ÷èñ -
ëå îïû òîâ // Óêð. áè î õèì. æóðí.—1975.—47, ¹ 6.—Ñ. 776—790.
22. Ïëî õèí ñêèé Í. À. Áè î ìåò ðèÿ.—Ì.: Èçä-âî ÌÃÓ, 1970.—367 ñ.
314
KATSAN V.A., POTOPALSKY A.I.
23. Ïî òî ïà ëüñêèé À. È., Êà öàí Â. À., Ëåñü êèâ Ì. Å. Âëè ÿ íèå ýê çî ãåí -
íûõ íà òèâ íûõ è ìî äè ôè öè ðî âàí íûõ íóê ëå è íî âûõ êèñ ëîò íà áè -
î ñèí òåç ôî òî ñèí òå òè ÷åñ êèõ ïèã ìåí òîâ ó Nicotiana tabacum L. 1.
Ñî äåð æà íèå õëî ðî ôèë ëîâ è êà ðî òè íî è äîâ ó ðàñ òå íèé ïåð âî ãî
ïî êî ëå íèÿ // Áè î ïî ëè ìå ðû è êëåò êà.—1995.—11, ¹ 2.—Ñ.
88—99.
24. Êà öàí Â. À., Ïî òî ïà ëüñêèé À. È. Âëè ÿ íèå ýê çî ãåí íûõ íà òèâ íûõ
è îäè ôè öè ðî âàí íûõ íóê ëå è íî âûõ êèñ ëîò íà áè î ñèí òåç ôî òî -
ñèí òå òè ÷åñ êèõ ïèã ìåí òîâ ó Nicotiana tabacum L. 2. Äè íà ìè êà ñî -
äåð æà íèÿ õëî ðî ôèë ëîâ è êà ðî òè íî è äîâ ó ðàñ òå íèé âòî ðî ãî è
òðåòü å ãî ïî êî ëå íèé // Áè î ïî ëè ìå ðû è êëåò êà.—2000.—16, ¹
1.—Ñ. 22—34.
25. Êà öàí Â. À., Ïî òî ïà ëüñêèé À. È., Þðêå âè÷ Ë. Í. Âè êî ðèñ òàí íÿ
åê çî ãåí íèõ ÄÍÊ äëÿ êî ðåêöi¿ ïðî ãðà ìè ðîç âèò êó æîâ òî ëèñ òî ãî
ìó òàí òó Nicotiana tabacum L. // Ôàê òî ðè åê ñïå ðè ìåí òàëü íî¿ åâî -
ëþöi¿ îðãàíiçìiâ.—Êè¿â: Àãðàðíà íà óêà, 2003.—Ñ. 339—345.
26. Ïî òî ïà ëüñêèé À. È., Êà öàí Â. À., Þðêå âè÷ Ë. Í. Èòî ãè è ïåð -
ñïåê òè âû ïî ëó ÷å íèÿ ðàñ òå íèé ñå ìå éñòâà ïàñ ëå íî âûõ ñ ïî -
ìîùüþ íà òèâ íûõ è ìî äè ôè öè ðî âàí íûõ ÄÍÊ // Îâî ùå âî äñòâî è
áàõ ÷å âî äñòâî.—2005.—51.—Ñ. 181—197.
27. Ma S., Gong Q., Bohnert H. J. Dissecting salt stress pathways // J.
Exp. Bot.—2006.—57.—P. 1097—1107.
28. Wu C. A., Yang G. D., Meng Q. W., Zheng C. C. The cotton GhNHX1
gene encoding a novel putative tonoplast Na+/H+ antiporter plays an
important role in salt stress // Plant Cell Physiol.—2004.—45.—P.
600—607.
29. Kishor K., Hong Z., Miao G.-H., Hu C.-A. A., Verma D. P. S.
Overexpression of pyrroline-5-carboxylate synthetese increases
proline production and confers osmotolerance in transgenic plants //
Plant Physiol.—1995.—108.—P. 1387—1394.
30. Holmstrom K.-O., Somersalo S., Mandal A., Palva T. E., Welin B.
Improved tolerance to salinity and low temperature in transgenic
tobacco producing glycine betaine // J. Exp. Bot.—2000.—51.—P.
177—185.
31. Jang I. C., Oh S. J., Seo J. S., Choi W. B., Song S. I., Kim C. H., Kim Y.
S., Seo H. S., Choi Y. D., Nahm B. H., Kim J. K. Expression of a
bifunctional fusion of a Escherichia coli genes for
tregalose-6-phosphate synthase and tregalose-6-phosphate
phosphatase in transgenic rice plants increases tregalose
accumulation and abiotic stress tolerance without stunting growth //
Plant Physiol.—2003.—131.—P. 516—524.
32. Das-Chatterjee A., Goswami L., Maitra S., Dastidar K. G., Ray S.,
Majumder A. L. Introgression as a novel salt-tolerant L-myo-inositol
1-phosphate synthase from Posterresia coarctata (Roxb.) Tateoka
(PcINO1) confers salt tolerance to evolutionary diverse organisms //
FEBS Lett.—2006.—580.—P. 3980—3988.
33. Wi S. G., Kim W. T., Park K. Y. Overexpression of carnation
S-adenosinmethionine decarboxylase gene generates a
broad-spectrum tolerance to abiotic stresses in transgenic tobacco
plants // Plant Cell Rep.—2006.—Epub. ahead of print.
34. Singla-Pareek S. L., Reddy M. K., Sopory S. K. Genetic engineering of
the glyoxalase pathway in tobacco leads to enhanced salinity
tolerance // Proc. Nat. Acad. Sci. USA.—2003.—100.—P.
14672—14677.
35. Sanan-Mishra N., Pham X. H., Sopory S. K., Tuteja N. Pea DNA
helicase 45 overexpression in tobacco confers high salinity tolerance
without affecting yield // Proc. Nat. Acad. Sci.
USA.—2005.—102.—P. 509—514.
36. Yen H. E., Edwards G. E., Grimes H. D. Characterization of a
salt-responsive 24-kilodalton glycoprotein in Mesembryanthemum
crystallinum // Plant Physiol.—1994.—105.—P. 1179—1187.
37. Yamasato A., Nagata N., Tanaka R., Tanaka A. The N-terminal
domain of chlorophyllide a oxygenase confers protein instability in
response to chlorophyll b accumulation in Arabidopsis // Plant
Cell.—2005.—17.—P. 1585—1597.
38. Pogson B., McDonald K., Tround M., Britton G., DellaPenna D.
Arabidopsis carotenoid mutants demonstrate that lutein is not
essential for photosynthesis in higher plants // Plant
Cell.—1996.—8.—P. 1627—1639.
39. Gonzalez-Guzman M., Abia D., Salinas J., Serrano L., Rodriguez P.
L. Two alleles of the abscisic oxygenase 3 gene reveals its role in
abscisic acid biosynthesis in seeds // Plant
Physiol.—2004.—135.—P. 325—333.
40. Espino E., Xiomara R., Garsia V., Garsia Y. H., Habana P. R. Nueva
variaded de tabaco negro (Nicotiana tabacum) con resistancia
multiple y buenas caracteristicas comerciales // Agrotech.
Cuba.—1989.—2.—P. 9—13.
41. Ýíåð ãå òè ÷åñ êèå àñ ïåê òû óñòîé ÷è âîñ òè ðàñ òå íèé / Ïîä ðåä. È. À.
Òàð ÷åâ ñêî ãî.—Êà çàíü: Èçä-âî Êà çàí. óí-òà, 1986.—140 ñ.
42. Õå ñèí Ð. Â. Íå ïîñ òî ÿ íñòâî ãå íî ìà.—Ì.: Íà ó êà, 1984.—472 ñ.
43. Êà öàí Â. À., Ïî òî ïà ëüñêèé À. È., Ëåñü êèâ Ì. Å. Óñòîé ÷è âîñòü ê
êà íà ìè öè íó ðàñ òå íèé òà áà êà ïðè âîç äå éñòâèè ïðå ïà ðà òîâ ÄÍÊ
íà ñå ìå íà // Áè î ïî ëè ìå ðû è êëåò êà.—1996.—12, ¹ 2.—Ñ.
47—55.
44. Jordan E. T., Hatfield P. M., Hondrid D., Talon M., Zeevart A. D.,
Viestra R. D. Phytochrome A overexpression in transgenic tobacco //
Plant Physiol.—1995.—107.—P. 797—805.
45. Peng J., Harberd N. P. Gibberellin deficiency and response mutations
suppress the stem elongation phenotype and phytochrome-deficient
mutants of Arabidopsis // Plant Physiol.—1997.—113.—P.
1051—1058.
46. Genoud T., Buchala A. J., Chua N. H., Metaux J. P. Phytochrome
signalling modulates the SA-perceptive pathway in Arabidopsis //
Plant J.—2002.—31.—P. 87—95.
47. Zeiter J., Pink B., Mueller B. J., Berger S. Light conditions influence
specific defence responses in incopatible plant-pathogen
interactions: uncoupling systemic resistance from salicylic acid and
PR-1 accumulation // Planta.—2004.—219.—P. 673—683.
48. Buchanan-Wollaston V., Page T., Harrison E., Breeze E., Lim P. O.,
Nam H. G., Lin J.-F., Wu S.-H., Swidzinski J., Ishizaki K., Leaver C.
Comparative transcriptome analysis reveals significant differences in
gene expression and signalling pathways between developmental and
dark/starvation induced senescence in Arabidopsis // Plant
J.—2005.—42.—P. 567—585.
49. Gibson S. I. Sugar and phytochrome response pathways: navigating a
signalling network // J. Exp. Bot.—2004.—55.—P. 253—264.
50. Monte E., Alonso J. M., Ecker J. R., Zhang Y., Li X., Young J.,
Austin-Philips S., Quail P. H. Isolation and characterization of phyC
mutants in Arabidopsis reveals complex crosstalk between
phytochrome signalling pathways // Plant Cell.—2003.—15.—P.
1962—1980.
51. McClintock B. The significance of responses of the genome to
challenge // Science.—1984.—226.—P. 792—801.
52. Ãå îð ãè åâ Ï. Ã. Ðîëü ìî áèëü íûõ ãå íå òè ÷åñ êèõ ýëå ìåí òîâ â ìó òà -
ãå íå çå, èí äó öè ðî âàí íîì õè ìè ÷åñ êè ìè è ôè çè ÷åñ êè ìè àãåí òà ìè:
Àâòîðåô. äèñ. ... êàíä. áèîë. íàóê / Èíñòè òóò ìî ëå êó ëÿð íîé áè î -
ëî ãèè èì. Í. Ã. Ýíãåëü ãàð äòà.—Ìîñ êâà, 1991.—23 ñ.
53. Ðàò íåð Â. À. Áëî÷ íî-ìî äóëü íûé ïðè íöèï îðãà íè çà öèè è ýâî ëþ -
öèè ìî ëå êó ëÿð íî-ãå íå òè ÷åñ êèõ ñèñ òåì óïðàâ ëå íèÿ (ÌÃÑÓ) //
Ãå íå òè êà.—1992.—28, ¹ 2.—Ñ. 5—24.
54. Ðàò íåð Â. À., Âà ñèëü å âà Ë. À. Ðîëü ìî áèëü íûõ ãå íå òè ÷åñ êèõ ýëå -
ìåí òîâ â ìèê ðî ý âî ëþ öèè // Ãå íå òè êà.—1992.—28, ¹ 12.—Ñ.
5—17.
55. Ðàò íåð Â. À., Âà ñèëü å âà Ë. À. Êðè òè ÷åñ êèå îãðà íè ÷å íèÿ ãå íîì -
íîé ñèñ òå ìû ìî áèëü íûõ ãå íå òè ÷åñ êèõ ýëå ìåí òîâ (ÌÃÝ) // Ãå íå -
òè êà.—1994.—30, ¹ 5.—Ñ. 593—599.
56. Ãâîç äåâ Â. À., Êàé äà íîâ Ë. Ç. Ñèñ òåì íûå èç ìå íå íèÿ ëî êà ëè çà öèè
ìî áèëü íûõ ýëå ìåí òîâ â ãå íî ìå Drosophila melanogaster, ñî ïðî -
âîæ äà þ ùèå ïðî öåñ ñû ñå ëåê öèè // Ìî ëå êó ëÿð íûå ìå õà íèç ìû ãå -
íå òè ÷åñ êèõ ïðî öåñ ñîâ.—Ì.: Íà ó êà, 1990.—Ñ. 26—36.
57. Áå ëÿ å âà Å. Ñ., Ïà ñþ êî âà Å. Ã., Ãâîç äåâ Â. À. “Àäàïòèâíûå òðàíñ -
ïî çè öèè” ðåò ðîò ðàí ñïî çî íîâ â ãå íî ìå Drosophila melanogaster,
ñî ïðî âîæ äà þ ùè å ñÿ óâå ëè ÷å íè åì ïðè ñïî ñîá ëåí íîñ òè îñî áåé //
Ãå íå òè êà.—1994.—30, ¹ 6.—Ñ. 725—730.
315
DNAS MAY INFLUENCE PLANT ADAPTATION REACTIONS
58. Ãåð øåí çîí Ñ. Ì. Ìó òà ãåí íîå äå éñòâèå ÄÍÊ è ïðî áëå ìà íà ïðàâ -
ëåí íûõ ìó òà öèé // Ãå íå òè êà.—1966.—¹ 5.—Ñ. 3—15.
59. Ãåð øåí çîí Ñ. Ì. Ìó òà ãåí íîå äå éñòâèå ÄÍÊ, èí ñåð öèè, òðàíñ ïî -
çè öèè è íå ñòà áèëü íûå ãåíû // Ãå íå òè êà è áëà ãî ñîñ òî ÿ íèå ÷å ëî -
âå ÷åñ òâà: Òð. XV Ìåæ äó íàð. ãå íåò. êîíãð.—Ì.: Íà ó êà,
1981.—Ñ. 304—318.
60. Ãåð øåí çîí Ñ. Ì., Àëåêñàíäðîâ Þ. Ì. Ìó òà ãåí íîå äå éñòâèå ïðè -
ðîä íûõ è ñèí òå òè ÷åñ êèõ ïî ëè íóê ëå î òè äîâ è ïðî áëå ìû íà ïðàâ -
ëåí íûõ ìó òà öèé // Æóðí. îáù. áè î ëî ãèè.—1982.—43, ¹ 6.—Ñ.
747—763.
61. Âà âè ëîâ Í. È. Çà êîí ãî ìî ëî ãè ÷åñ êèõ ðÿ äîâ â íà ñëå äñòâåí íîé èç -
ìåí ÷è âîñ òè.—Ëå íèí ãðàä: Íà ó êà, 1987.—260 ñ.
ÓÄÊ 575.224:577.113
Íàäiéøëà äî ðå äàêöi¿ 19.06.06
316
KATSAN V.A., POTOPALSKY A.I.
|
| id | nasplib_isofts_kiev_ua-123456789-156776 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 0233-7657 |
| language | Ukrainian |
| last_indexed | 2025-12-07T16:09:35Z |
| publishDate | 2006 |
| publisher | Інститут молекулярної біології і генетики НАН України |
| record_format | dspace |
| spelling | Кацан, В.А. Потопальський, А.І. 2019-06-18T20:22:39Z 2019-06-18T20:22:39Z 2006 Екзогенні ДНК можуть впливати на регуляторні системи рослин, відповідальні за адаптацію до змін у довкіллі / В.А. Кацан, А.І. Потопальський // Біополімери і клітина. — 2006. — Т. 22, № 4. — С. 307-316. — Бібліогр.: 61 назв. — укр., англ. 0233-7657 DOI: http://dx.doi.org/10.7124/bc.00073D https://nasplib.isofts.kiev.ua/handle/123456789/156776 575.224:577.113 Розроблено оригінальну технологію отримання рослин тютюну з комплексом селекційно цінних ознак (прискорений розвиток, висока продуктивність та стійкість до комплексного засолення грунтів) за допомогою препаратів екзогенної ДНК (е-ДНК), вивчено фізіолого-біохімічні особливості таких рослин та їхнє спадкування. Для індукування бажаних змін у жовтолистого сорту тютюну Крупнолистный 20 (КР20) використано ДНК солестійкої форми пасльону чорного Solanum nigrum L. і плазмід pCAMVNEO та рТі8628. Важлива її перевага — забезпечення ширшого спектра змін і більшого виходу змінених життєздатних рослин. На основі аналізу модифікацій, виявлених у тютюну та рослин модельної системи, використаних при розробці технології, запропоновано гіпотетичний механізм впливу е-ДНК на спадковість рослин. Разработана оригинальная технология получения растений табака, обладающих комплексом селекционно ценных призна ков (ускоренное развитие, повышенная продуктивность, устойчивость к комплексному засолению почвы) при помощи препаратов экзогенных ДНК (э-ДНК), изучены физиолого-биохимические особенности таких растений и их наследование. Для индуцирования желаемых изменений у желтолистного сорта табака Крупнолистный 20 использована ДНК солеустойчивой формы паслена черного Solanum nigrum L., а также ДНК плазмид pCAMVNEO и pTi8628. Важное преимущество разработанной технологии — обеспечение более широкого спектра изменений и более значительный выход измененных жизнеспособных растений. На основе анализа модификаций, обнаруженных у табака и растений модельной системы, использованных при разработке технологии, предложен гипотетический механизм влияния э-ДНК на наследтвенные признаки растений. The original technology of obtaining the tobacco plants possessing the complex of selectively useful features (accelerated development, high productivity, and resistance to complex salinization) has been elaborated, and inheritance of the physiological and biochemical peculiarities of such plants have been investigated. To get the important and selective changes of yellow-leaved tobacco Krupnolistny 20 (KR 20, Large-leaved 20) cultivar, the native and alkylated by thiophosphamide DNA of salt-tolerant nightshade (Solanum nigrum L.) and DNA of pCAMVNEO and pTi8628 plasmids have been used. The valuable advantage of such technology is the provision of a wider range of changes and larger output of changed viable plants. The changes obtained by DNA action on tobacco and other plants exploited in technology elaboration have been analysed, and possible mechanism of exogenic DNAs influence on plant heredity has been proposed. uk Інститут молекулярної біології і генетики НАН України Біополімери і клітина Геном та його регуляція Екзогенні ДНК можуть впливати на регуляторні системи рослин, відповідальні за адаптацію до змін у довкіллі Экзогенные ДНК могут влиять на регуляторные системы растений, отвечающие за адаптацию к изменениям в окружающей среде Exogenic DNAs may influence plant adaptation reactions to changed environment Article published earlier |
| spellingShingle | Екзогенні ДНК можуть впливати на регуляторні системи рослин, відповідальні за адаптацію до змін у довкіллі Кацан, В.А. Потопальський, А.І. Геном та його регуляція |
| title | Екзогенні ДНК можуть впливати на регуляторні системи рослин, відповідальні за адаптацію до змін у довкіллі |
| title_alt | Экзогенные ДНК могут влиять на регуляторные системы растений, отвечающие за адаптацию к изменениям в окружающей среде Exogenic DNAs may influence plant adaptation reactions to changed environment |
| title_full | Екзогенні ДНК можуть впливати на регуляторні системи рослин, відповідальні за адаптацію до змін у довкіллі |
| title_fullStr | Екзогенні ДНК можуть впливати на регуляторні системи рослин, відповідальні за адаптацію до змін у довкіллі |
| title_full_unstemmed | Екзогенні ДНК можуть впливати на регуляторні системи рослин, відповідальні за адаптацію до змін у довкіллі |
| title_short | Екзогенні ДНК можуть впливати на регуляторні системи рослин, відповідальні за адаптацію до змін у довкіллі |
| title_sort | екзогенні днк можуть впливати на регуляторні системи рослин, відповідальні за адаптацію до змін у довкіллі |
| topic | Геном та його регуляція |
| topic_facet | Геном та його регуляція |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/156776 |
| work_keys_str_mv | AT kacanva ekzogennídnkmožutʹvplivatinaregulâtornísistemiroslinvídpovídalʹnízaadaptacíûdozmínudovkíllí AT potopalʹsʹkiiaí ekzogennídnkmožutʹvplivatinaregulâtornísistemiroslinvídpovídalʹnízaadaptacíûdozmínudovkíllí AT kacanva ékzogennyednkmogutvliâtʹnaregulâtornyesistemyrasteniiotvečaûŝiezaadaptaciûkizmeneniâmvokružaûŝeisrede AT potopalʹsʹkiiaí ékzogennyednkmogutvliâtʹnaregulâtornyesistemyrasteniiotvečaûŝiezaadaptaciûkizmeneniâmvokružaûŝeisrede AT kacanva exogenicdnasmayinfluenceplantadaptationreactionstochangedenvironment AT potopalʹsʹkiiaí exogenicdnasmayinfluenceplantadaptationreactionstochangedenvironment |