Influence of chloroquine on kinetics of single-cell gel electrophoresis

• Published in: Вiopolymers and Cell
• Date: 2012
• Main Authors: Zazhytska, M.O., Afanasieva, K.S., Chopei, M.I., Vikhreva, M.A., Sivolob, A.V.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2012
• Subjects: Structure and Function of Biopolymers
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/156929
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Influence of chloroquine on kinetics of single-cell gel electrophoresis / M.O. Zazhytska, K.S. Afanasieva, M.I. Chopei, M.A. Vikhreva, A.V. Sivolob // Вiopolymers and Cell. — 2012. — Т. 28, № 4. — С. 292–297. — Бібліогр.: 20 назв. — англ.

In single-cell gel electrophoresis (the comet assay) the DNA of lysed cells, the nucleoids, extends towards the anode in a track resembling a comet tail. The aim of this work was to investigate the effects of changes in DNA topology on this process. Methods. We used the kinetic approach, proposed ea...