The level of blood plasma mitochondrial DNA upon acute myocardium damage in experiment
The aim of the present investigation is to study the level of plasma mtDNA as a potential marker of cardiomyocyte damage in 2 and 4 h after subcutaneous injection of adrenaline and during the formed morphological alterations of the myocardium (3 days). Methods. Real time PCR. Male Wistar rats were u...
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| Cite this: | The level of blood plasma mitochondrial DNA upon acute myocardium damage in experiment / N.P. Sudakov, T.P. Popkova, M.A. Novikova, A.I. Katyshev, S.B. Nikiforov, B.G. Pushkarev, O.A. Goldberg, I.B. Klimenkov, S.A. Lepekhova, S.D. Ezhikeeva, M.N. Ten, V.G. Osipov, Yu.M. Konstantinov // Вiopolymers and Cell. — 2012. — Т. 28, № 4. — С. 322-325. — Бібліогр.: 7 назв. — англ. |
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Sudakov, N.P. Popkova, T.P. Novikova, M.A. Katyshev, A.I. Nikiforov, S.B. Pushkarev, B.G. Goldberg, O.A. Klimenkov, I.B. Lepekhova, S.A. Ezhikeeva, S.D. Ten, M.N. Osipov, V.G. Konstantinov, Yu.M. 2019-06-19T11:01:28Z 2019-06-19T11:01:28Z 2012 The level of blood plasma mitochondrial DNA upon acute myocardium damage in experiment / N.P. Sudakov, T.P. Popkova, M.A. Novikova, A.I. Katyshev, S.B. Nikiforov, B.G. Pushkarev, O.A. Goldberg, I.B. Klimenkov, S.A. Lepekhova, S.D. Ezhikeeva, M.N. Ten, V.G. Osipov, Yu.M. Konstantinov // Вiopolymers and Cell. — 2012. — Т. 28, № 4. — С. 322-325. — Бібліогр.: 7 назв. — англ. 0233-7657 DOI: http://dx.doi.org/10.7124/bc.00006B https://nasplib.isofts.kiev.ua/handle/123456789/156936 577.21 The aim of the present investigation is to study the level of plasma mtDNA as a potential marker of cardiomyocyte damage in 2 and 4 h after subcutaneous injection of adrenaline and during the formed morphological alterations of the myocardium (3 days). Methods. Real time PCR. Male Wistar rats were used as the experimental animals. Results. It was shown that during the increase in the activity of cytolysis biomarkers, at the first hours after adrenaline injection, no reliable increase is observed in the level of free circulating blood mtDNA. A tendency of 2.5-fold increase in this parameter was established at the third day after adrenaline injection during the development of acute inflammatory process in the myocardium. On the whole, further researches are needed on the dynamics of mtDNA level upon acute damage of the myocardium in experimental and clinical investigations for unbiased estimation of the prospects of using the parameter in laboratory diagnostics. Keywords: mitochondrial DNA, cardiovascular diseases, adrenaline myocarditis, cytolysis biomarkers. Цель. Изучить уровень мтДНК плазмы крови как возможного маркера повреждений кардиомиоцитов через 2 и 4 ч после подкожной инъекции адреналина и на фоне сформированных морфологических изменений миокарда (3-и сут). Методы. Полимеразная цепная реакция в реальном времени. В экспериментах использовали самцов крыс линии Вистар. Результаты. Показано, что наряду с увеличением активности биомаркеров цитолиза в первые часы после введения адреналина значимого повышения уровня свободно циркулирующей мтДНК крови не происходит. Установлена тенденция к 2,5-кратному возрастанию данного показателя на 3-и сут после инъекции адреналина на фоне развития острого воспалительного процесса в миокарде. Выводы. В целом для объективной оценки перспектив этого показателя в лабораторной диагностике инфаркта миокарда необходимо дальнейшее изучение динамики уровня мтДНК при острых повреждениях миокарда в экспериментальных и клинических исследованиях. Ключевые слова: митохондриальная ДНК, сердечно-сосудистые заболевания, адреналиновый миокардит, биомаркеры цитолиза. Мета. Вивчити рівень мтДНК плазми крові як можливого маркера пошкоджень кардіоміоцитиів через 2 і 4 год після підшкірної ін’єкції адреналіну та на фоні сформованих морфологічних змін міокарда (3-тя доба). Методи. Полімеразна ланцюгова реакція у реальному часі. В експериментах використано самців щурів лінії Вістар. Результати. Показано, що поряд із збільшенням активності біомаркерів цитолізу в перші години після введення адреналіну суттєвого підвищення рівня вільно циркулюючої мтДНК крові не відбувається. Встановлено тенденцію до 2,5-разового зростання даного показника на 3-тю добу після ін’кції адреналіну на фоні розвитку гострого запального процесу в міокарді. Висновки. У цілому для об’єктивної оцінки перспектив цього показника у лабораторній діагностиці інфаркта міокарда необхідно подальше вивчення динаміки рівня мтДНК при гострих ураженнях міокарда в експериментальних і клінічних дослідженнях. Ключові слова: мітохондріальна ДНК, серцево-судинні захворювання, адреналіновий міокардит, біомаркери цитолізу. en Інститут молекулярної біології і генетики НАН України Вiopolymers and Cell Short Communications The level of blood plasma mitochondrial DNA upon acute myocardium damage in experiment Рівень мітохондріальної ДНК плазми крові за гострого пошкодження міокарда в експерименті Уровень митохондриальной ДНК плазмы крови при остром повреждении миокарда в эксперименте Article published earlier |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| title |
The level of blood plasma mitochondrial DNA upon acute myocardium damage in experiment |
| spellingShingle |
The level of blood plasma mitochondrial DNA upon acute myocardium damage in experiment Sudakov, N.P. Popkova, T.P. Novikova, M.A. Katyshev, A.I. Nikiforov, S.B. Pushkarev, B.G. Goldberg, O.A. Klimenkov, I.B. Lepekhova, S.A. Ezhikeeva, S.D. Ten, M.N. Osipov, V.G. Konstantinov, Yu.M. Short Communications |
| title_short |
The level of blood plasma mitochondrial DNA upon acute myocardium damage in experiment |
| title_full |
The level of blood plasma mitochondrial DNA upon acute myocardium damage in experiment |
| title_fullStr |
The level of blood plasma mitochondrial DNA upon acute myocardium damage in experiment |
| title_full_unstemmed |
The level of blood plasma mitochondrial DNA upon acute myocardium damage in experiment |
| title_sort |
level of blood plasma mitochondrial dna upon acute myocardium damage in experiment |
| author |
Sudakov, N.P. Popkova, T.P. Novikova, M.A. Katyshev, A.I. Nikiforov, S.B. Pushkarev, B.G. Goldberg, O.A. Klimenkov, I.B. Lepekhova, S.A. Ezhikeeva, S.D. Ten, M.N. Osipov, V.G. Konstantinov, Yu.M. |
| author_facet |
Sudakov, N.P. Popkova, T.P. Novikova, M.A. Katyshev, A.I. Nikiforov, S.B. Pushkarev, B.G. Goldberg, O.A. Klimenkov, I.B. Lepekhova, S.A. Ezhikeeva, S.D. Ten, M.N. Osipov, V.G. Konstantinov, Yu.M. |
| topic |
Short Communications |
| topic_facet |
Short Communications |
| publishDate |
2012 |
| language |
English |
| container_title |
Вiopolymers and Cell |
| publisher |
Інститут молекулярної біології і генетики НАН України |
| format |
Article |
| title_alt |
Рівень мітохондріальної ДНК плазми крові за гострого пошкодження міокарда в експерименті Уровень митохондриальной ДНК плазмы крови при остром повреждении миокарда в эксперименте |
| description |
The aim of the present investigation is to study the level of plasma mtDNA as a potential marker of cardiomyocyte damage in 2 and 4 h after subcutaneous injection of adrenaline and during the formed morphological alterations of the myocardium (3 days). Methods. Real time PCR. Male Wistar rats were used as the experimental animals. Results. It was shown that during the increase in the activity of cytolysis biomarkers, at the first hours after adrenaline injection, no reliable increase is observed in the level of free circulating blood mtDNA. A tendency of 2.5-fold increase in this parameter was established at the third day after adrenaline injection during the development of acute inflammatory process in the myocardium. On the whole, further researches are needed on the dynamics of mtDNA level upon acute damage of the myocardium in experimental and clinical investigations for unbiased estimation of the prospects of using the parameter in laboratory diagnostics.
Keywords: mitochondrial DNA, cardiovascular diseases, adrenaline myocarditis, cytolysis biomarkers.
Цель. Изучить уровень мтДНК плазмы крови как возможного маркера повреждений кардиомиоцитов через 2 и 4 ч после подкожной инъекции адреналина и на фоне сформированных морфологических изменений миокарда (3-и сут). Методы. Полимеразная цепная реакция в реальном времени. В экспериментах использовали самцов крыс линии Вистар. Результаты. Показано, что наряду с увеличением активности биомаркеров цитолиза в первые часы после введения адреналина значимого повышения уровня свободно циркулирующей мтДНК крови не происходит. Установлена тенденция к 2,5-кратному возрастанию данного показателя на 3-и сут после инъекции адреналина на фоне развития острого воспалительного процесса в миокарде. Выводы. В целом для объективной оценки перспектив этого показателя в лабораторной диагностике инфаркта миокарда необходимо дальнейшее изучение динамики уровня мтДНК при острых повреждениях миокарда в экспериментальных и клинических исследованиях.
Ключевые слова: митохондриальная ДНК, сердечно-сосудистые заболевания, адреналиновый миокардит, биомаркеры цитолиза.
Мета. Вивчити рівень мтДНК плазми крові як можливого маркера пошкоджень кардіоміоцитиів через 2 і 4 год після підшкірної ін’єкції адреналіну та на фоні сформованих морфологічних змін міокарда (3-тя доба). Методи. Полімеразна ланцюгова реакція у реальному часі. В експериментах використано самців щурів лінії Вістар. Результати. Показано, що поряд із збільшенням активності біомаркерів цитолізу в перші години після введення адреналіну суттєвого підвищення рівня вільно циркулюючої мтДНК крові не відбувається. Встановлено тенденцію до 2,5-разового зростання даного показника на 3-тю добу після ін’кції адреналіну на фоні розвитку гострого запального процесу в міокарді. Висновки. У цілому для об’єктивної оцінки перспектив цього показника у лабораторній діагностиці інфаркта міокарда необхідно подальше вивчення динаміки рівня мтДНК при гострих ураженнях міокарда в експериментальних і клінічних дослідженнях.
Ключові слова: мітохондріальна ДНК, серцево-судинні захворювання, адреналіновий міокардит, біомаркери цитолізу.
|
| issn |
0233-7657 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/156936 |
| citation_txt |
The level of blood plasma mitochondrial DNA upon acute myocardium damage in experiment / N.P. Sudakov, T.P. Popkova, M.A. Novikova, A.I. Katyshev, S.B. Nikiforov, B.G. Pushkarev, O.A. Goldberg, I.B. Klimenkov, S.A. Lepekhova, S.D. Ezhikeeva, M.N. Ten, V.G. Osipov, Yu.M. Konstantinov // Вiopolymers and Cell. — 2012. — Т. 28, № 4. — С. 322-325. — Бібліогр.: 7 назв. — англ. |
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322
UDC 577.21
The level of blood plasma mitochondrial DNA upon
acute myocardium damage in experiment
N. P. Sudakov1, 3, T. P. Popkova3, M. A. Novikova1, A. I. Katyshev2, S. B. Nikiforov1,
B. G. Pushkarev1, O. A. Goldberg1, I. B. Klimenkov4, 6, S. A. Lepekhova1,
S. D. Ezhikeeva3, M. N. Ten3, V. G. Osipov5, Yu. M. Konstantinov2, 6
1Scientific Center for Reconstructive and Restorative Surgery, Siberian Branch, Russian Academy of Medical Sciences
1, Bortsov Revolyutsii Str., Irkutsk, Russian Federation, 664003
2Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences
132, Lermontova Str., Irkutsk, Russian Federation, 664033
3Regional Clinical Hospital
100, m-n Yubileinyi, Irkutsk, Russian Federation, 66409
4Limnological Institute, Siberian Branch of the Russian Academy of Sciences
3, Ulan-Batorskaya Str., Irkutsk, Russian Federation, 665033
5Territorial Center of Catastrophe Medicine
100 k3, m-n Yubileinyi, Irkutsk, Russian Federation, 664049
6Irkutsk State University
1, Karl Marks Str., Irkutsk, Russian Federation, 664003
npsudakov@rambler.ru; yukon@sifibr.irk.ru
The aim of the present investigation is to study the level of plasma mtDNA as a potential marker of cardiomyo-
cyte damage in 2 and 4 h after subcutaneous injection of adrenaline and during the formed morphological al-
terations of the myocardium (3 days). Methods. Real time PCR. Male Wistar rats were used as the experimental
animals. Results. It was shown that during the increase in the activity of cytolysis biomarkers, at the first hours
after adrenaline injection, no reliable increase is observed in the level of free circulating blood mtDNA. A ten-
dency of 2.5-fold increase in this parameter was established at the third day after adrenaline injection during the
development of acute inflammatory process in the myocardium. On the whole, further researches are needed on
the dynamics of mtDNA level upon acute damage of the myocardium in experimental and clinical investigations
for unbiased estimation of the prospects of using the parameter in laboratory diagnostics.
Keywords: mitochondrial DNA, cardiovascular diseases, adrenaline myocarditis, cytolysis biomarkers.
Introduction. Cardiovascular diseases are currently one
of the major causes of mortality incidence increase in
developed countries. The efficiency of prevention and
therapy of acute ischemic damage of the myocardium
is determined by the potential of diagnostic screening
and monitoring techniques [1]. The utilization of the
currently known range of cytolysis biomarkers does not
always allow definite determination of patient state and
cardiac infarction cure option selection [2]. Therefore,
the search for novel cardiomyocyte damage biomarkers
and their pathological basis is an urgent task [1]. A pro-
mising nonspecific marker of cytological processes is the
level of free circulating mitochondrial DNA (mtDNA)
of blood plasma. The level of free and cellular blood
mtDNA was established to be a criterion for disease
complication and mortality prognosis in different patho-
logical processes (tumors, bacterial meningitis) [3–5].
Currently, the dynamics of this parameter remains un-
explored for cardiovascular diseases. Despite the lack of
tissue specificity, the possibility to utilize this parame-
ISSN 0233–7657. Biopolymers and Cell. 2012. Vol. 28. N 4. P. 322–325
Ó Institute of Molecular Biology and Genetics, NAS of Ukraine, 2012
323
THE LEVEL OF BLOOD PLASMA MITOCHONDRIAL DNA UPON ACUTE MYOCARDIUM DAMAGE
ter in addition to the cardiospecific cytolysis biomar-
kers for acute and chronic cardiovascular disease diag-
nostics and prognosis is of scientific and practical inte-
rest. Therefore, the aim of the present research is to in-
vestigate the level of plasma mtDNA as a potential mar-
ker of cardiomyocyte damage during the first hours of
adrenaline myocarditis modeling and upon developed
morphological alterations in the myocardium.
Materials and methods. Male Wistar rats were used
as the experimental animals that were divided into two
groups: 1) control (n = 6, subcutaneous injection of the
physiological solution); 2) experimental myocarditis
(n = 18, subcutaneous injection of 0.2 mg of adrenaline
hydrochloride per 100 g of animal body weight). The
animals were sacrificed in 2 h (n = 6), 4 h (n = 6), and 3
days (n = 6); blood was collected and autopsy was per-
formed [6].
Blood of the experimental animals stabilized with
nitrate citrate was used to obtain blood plasma free of
thrombocytes [3]. DNA was extracted from blood using
the DNA-probe reagent kit («DNA-technology», Russia).
Quantitative analysis of mtDNA was performed using
the real time PCR approach with the iCycler IQ4 amp-
lifier («Bio-Rad», USA) and DTlite («DNA-technolo-
gy»). The 16S RNA fragment (direct primer: 5'-TGCA
GAAGCTATTAATGGTTCG-3', reverse primer: 5'-T
TGGCTCTGCCACCCTAATA-3') was amplified [4].
A reaction mixture containing SYBR Green (MaximaTM
SYBR Green/ROX qPCR Master Mix – «Fermentas»,
Lithuania) was used for real time PCR. All the manipu-
lations with the experimental animals were conducted
according to the regulations for experimental research
(Ministry of Health, Soviet Union, Order of August 12,
1977, N 755). The results were analyzed using non-pa-
rametric statistics and median, and quartiles were calcu-
lated; intergroup differences were assessed with the
Mann-Whitney and Kraskel-Wallace criteria.
Results and discussion. In 2 h after adrenaline in-
jection, statistically reliable increase (p £ 0.05) was re-
corded in the activity of cytolysis biomarker enzymes:
creatine kinase (120 %), creatine phosphokinase-MB
(150 %), lactate dehydrogenase (130 %), and aspartate
transaminase (140 %) in the blood of the experimental
animals. The values of creatine kinase, lactate dehyd-
rogenase, and aspartate transaminase activity continued
to increase reliably for 4 h (Fig. 1). The data obtained
definitely evidence to the occurrence of cardiomyocyte
damage after adrenaline injection at these points of the
IU/L A B
C D
0 2 4 72 h
0 2 4 72 h 0 2 4 72 h
0 2 4 72 h
IU /L
IU/L
IU /L
2000
4000
6000
8000
1000
2000
3000
0 0
50
100
150
200
250
0 0
1000
2000
3000
Fig. 1. Activity of cytolysis biomarker enzymes during adrenaline myocarditis: A – creatine kinase; B – creatine phosphokinase-MB; C – lactate
dehydrogenase; D – aspartate transaminase; *p £ 0.05 versus control group
324
SUDAKOV N. P. ET AL.
experiment. The level of free circulating plasma mtDNA
had no statistically reliable difference from the control
group in 2 and 4 h after adrenaline injection (Fig. 2).
In 3 days, the myocardium of the animals with adre-
naline injections was characterized by marked myocar-
ditis symptoms: multiple lysis focuses of muscle fibers
infiltrated with macrophages were observed on the pre-
parations stained with the hematoxylin and eosin (Fig.
3,). The focuses of inflammation were found both in
the central part of the myocardium and in direct contact
with the endocardium and pericardium. Myocardium
infiltration with macrophages was focal in some cases.
In the experimental group, the activity of cytolysis
biomarkers decreased and virtually had no difference
from the values of the control group at this period. Ne-
vertheless, it was registered 2.5 fold increase in free
plasma mtDNA level in the experimental animals as
compared to the control subjects under 3 days of experi-
mental myocarditis modeling (p = 0.16). It is likely that
detailed analysis of mtDNA level dynamics is needed
between 4 and 72 h of myocarditis modeling as well as at
later periods after adrenaline injection for unbiased in-
terpretation of the obtained data.
Thus, during the first hours after adrenaline injec-
tion, no reliable increase occurs in the level of free circu-
lating mtDNA, due to its potential efflux from the dama-
ged tissues. A tendency was observed of increase in this
parameter at the third day after injection during acute in-
flammatory process development in the myocardium.
Conclusion. The data obtained evidence to the fact
that despite strong efflux of cytolysis biomarkers from
the damaged myocardium in 2 and 4 h after subcutane-
ous adrenaline injection, no increase occurs in the level
of free circulating blood mtDNA of the experimental
animals. This fact excludes the possibility to employ this
parameter for cell damage monitoring at these periods of
adrenaline myocarditis. Relatively insignificant increase
in this parameter is observed at the third day after adre-
naline injection upon a decrease in cytolysis marker ac-
tivity and the development of acute inflammation pro-
cess in the myocardium. Possible explanation for the in-
crease in mtDNA level observed at the third day of the
experiment is mtDNA release from the polymorpho-
nuclear phagocytes in the form of extracellular traps,
when inflammation is activated in the damaged myocar-
dium [7]. This possibility is of interest for the construc-
tion of techniques for inflammation process after acute
ischemic myocardium damage and requires further in-
vestigations. It is undoubtedly that the characterized
points of adrenaline myocarditis development are not
sufficient for the objective representation of possible alte-
rations in mtDNA level. One more probable cause of
the obtained results can be the insufficiency of myocar-
dium damage induced in this experiment that predeter-
mines to perform ligation of coronary arteries.
All the above stated facts make it urgent to explore
in detail the dynamics of this parameter during acute da-
mage and inflammatory processes in the myocardium
in experiment and clinics that will later favor the deve-
lopment of novel techniques for acute myocardium da-
mage and diagnostics.
0 2 4 72 h
0
1
2
3
4
5
Copie s/ml
· 106
Fig. 2. Alterations in the level of free circulating blood mtDNA during
adrenaline myocarditis; *p = 0.16 versus control group
Fig. 3. Development of morphological alterations in the myocardium
at the third day after adrenaline injection. The lysis focuses of myo-
cardium fibers infiltrated with macrophages (arrow)
Acknowledgements. This work was financially sup-
ported by the Presidium RAS Program «Fundamental
Science for Medicine» (project FNM-16) and Russian
Fund for Basic Research (09-04-01341, 12-04-01400).
Í. Ï. Ñó äà êîâ, Ò. Ï. Ïîï êî âà, Ì. À. Íîâ³êîâà, À. ². Êà òè øåâ,
Ñ. Á. Íè êè ôî ðîâ, Á. Ã. Ïóø êà ðåâ, Î. À. Ãî ëüäáåðã,
². Â. Êëè ìåí êîâ, Ñ. À. Ëå ïå õî âà, Ñ. Ä. ªæèêåºâà, Ì. Í. Òåí,
Â. Ã. Îñè ïîâ, Þ. Ì. Êîí ñòàí òè íîâ
гâåíü ì³òî õîíäð³àëü íî¿ ÄÍÊ ïëàç ìè êðîâ³ çà ãîñ òðî ãî
ïî øêîä æåí íÿ ì³îêàð äà â åê ñïå ðè ìåíò³
Ðå çþ ìå
Ìåòà. Âèâ ÷è òè ð³âåíü ìòÄÍÊ ïëàç ìè êðîâ³ ÿê ìîæ ëè âî ãî ìàð êå -
ðà ïî øêîä æåíü êàðä³îì³îöè òè³â ÷å ðåç 2 ³ 4 ãîä ï³ñëÿ ï³äøê³ðíî¿
³í’ºêö³¿ àä ðå íàë³íó òà íà ôîí³ ñôîð ìî âà íèõ ìîð ôî ëîã³÷íèõ çì³í
ì³îêàð äà (3-òÿ äîáà). Ìå òî äè. Ïîë³ìå ðàç íà ëàí öþ ãî âà ðå àêö³ÿ ó
ðå àëü íî ìó ÷àñ³.  åê ñïå ðè ìåí òàõ âè êî ðèñ òà íî ñàìö³â ùóð³â ë³í³¿
³ñòàð. Ðå çóëü òà òè. Ïî êà çà íî, ùî ïî ðÿä ³ç çá³ëüøåí íÿì àê òèâ íî-
ñò³ á³îìàð êåð³â öè òîë³çó â ïåðø³ ãî äè íè ï³ñëÿ ââå äåí íÿ àä ðå íàë³íó
ñóòòºâîãî ï³äâè ùåí íÿ ð³âíÿ â³ëüíî öèð êó ëþ þ ÷î¿ ìòÄÍÊ êðîâ³ íå
â³äáó âàºòüñÿ. Âñòà íîâ ëå íî òåí äåíö³þ äî 2,5-ðàç îâî ãî çðîñ òàí íÿ
äà íî ãî ïî êàç íè êà íà 3-òþ äîáó ï³ñëÿ ³í’êö³¿ àä ðå íàë³íó íà ôîí³ ðîç -
âèò êó ãîñ òðî ãî çà ïàëü íî ãî ïðî öå ñó â ì³îêàðä³. Âèñ íîâ êè. Ó ö³ëîìó
äëÿ îá’ºêòèâ íî¿ îö³íêè ïåð ñïåê òèâ öüî ãî ïî êàç íè êà ó ëà áî ðà òîð-
í³é ä³àã íîñ òèö³ ³íôàð êòà ì³îêàð äà íå îáõ³äíî ïîä àëü øå âèâ ÷åí íÿ
äè íàì³êè ð³âíÿ ìòÄÍÊ ïðè ãîñ òðèõ óðà æåí íÿõ ì³îêàð äà â åê ñïå -
ðè ìåí òàëü íèõ ³ êë³í³÷íèõ äîñë³äæåí íÿõ.
Êëþ ÷îâ³ ñëî âà: ì³òî õîíäð³àëü íà ÄÍÊ, ñåð öå âî-ñó äèíí³ çà õâî -
ðþ âàí íÿ, àä ðå íàë³íî âèé ì³îêàð äèò, á³îìàð êå ðè öè òîë³çó.
Í. Ï. Ñó äà êîâ, Ò. Ï. Ïîï êî âà, Ì. À. Íî âè êî âà, À. È. Êà òû øåâ,
Ñ. Á. Íè êè ôî ðîâ, Á. Ã. Ïóø êà ðåâ, Î. À. Ãî ëüäáåðã,
È. Â. Êëè ìåí êîâ, Ñ. À. Ëå ïå õî âà, Ñ. Ä. Åæè êå å âà, Ì. Í. Òåí,
Â. Ã. Îñè ïîâ, Þ. Ì. Êîí ñòàí òè íîâ
Óðî âåíü ìè òî õîí äðè àëü íîé ÄÍÊ ïëàç ìû êðî âè ïðè îñòðîì
ïî âðåæ äå íèè ìè î êàð äà â ýêñ ïå ðè ìåí òå
Ðå çþ ìå
Öåëü. Èçó ÷èòü óðî âåíü ìòÄÍÊ ïëàç ìû êðî âè êàê âîç ìîæ íî ãî
ìàð êå ðà ïî âðåæ äå íèé êàð äè î ìè î öè òîâ ÷å ðåç 2 è 4 ÷ ïî ñëå ïîä -
êîæ íîé èíú åê öèè àä ðå íà ëè íà è íà ôîíå ñôîð ìè ðî âàí íûõ ìîð ôî-
ëî ãè ÷åñ êèõ èç ìå íå íèé ìè î êàð äà (3-è ñóò). Ìå òî äû. Ïî ëè ìå ðàç íàÿ
öåï íàÿ ðå àê öèÿ â ðå àëü íîì âðå ìå íè. Â ýêñ ïå ðè ìåí òàõ èñ ïîëü çî âà -
ëè ñàì öîâ êðûñ ëè íèè Âèñ òàð. Ðå çóëü òà òû. Ïî êà çà íî, ÷òî íà ðÿ äó
ñ óâå ëè ÷å íè åì àê òèâ íîñ òè áè î ìàð êå ðîâ öè òî ëè çà â ïåð âûå ÷àñû
ïî ñëå ââå äå íèÿ àä ðå íà ëè íà çíà ÷è ìî ãî ïî âû øå íèÿ óðîâ íÿ ñâî áîä íî
öèð êó ëè ðó þ ùåé ìòÄÍÊ êðî âè íå ïðî èñ õî äèò. Óñòà íîâ ëåíà òåí -
äåí öèÿ ê 2,5-êðàò íî ìó âîç ðàñ òà íèþ äàí íî ãî ïî êà çà òå ëÿ íà 3-è
ñóò ïî ñëå èíú åê öèè àä ðå íà ëè íà íà ôîíå ðàç âè òèÿ îñòðî ãî âîñ-
ïà ëè òåëü íî ãî ïðî öåñ ñà â ìè î êàð äå. Âû âî äû. Â öå ëîì äëÿ îá ú åê -
òèâ íîé îöåí êè ïåð ñïåê òèâ ýòî ãî ïî êà çà òå ëÿ â ëà áî ðà òîð íîé äè-
àã íîñ òè êå èí ôàð êòà ìè î êàð äà íå îá õî äè ìî äàëü íåé øåå èç ó÷å íè -
åï äè íà ìè êè óðîâ íÿ ìòÄÍÊ ïðè îñòðûõ ïî âðåæ äå íè ÿõ ìè î êàð äà
â ýêñ ïå ðè ìåí òàëü íûõ è êëè íè ÷åñ êèõ èñ ñëå äî âà íè ÿõ.
Êëþ ÷å âûå ñëî âà: ìè òî õîí äðè àëü íàÿ ÄÍÊ, ñåð äå÷ íî-ñî ñó äè-
ñòûå çà áî ëå âà íèÿ, àä ðå íà ëè íî âûé ìè î êàð äèò, áè î ìàð êå ðû öè -
òî ëè çà.
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Received 10.05.11
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THE LEVEL OF BLOOD PLASMA MITOCHONDRIAL DNA UPON ACUTE MYOCARDIUM DAMAGE
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