The Shine-Dalgarno hybrid during initiation of translation and elongation

• Published in: Біополімери і клітина
• Date: 2007
• Main Authors: Maraschio, D., La Teana, A., Volpe, P.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2007
• Subjects: Матеріали семінару
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/157528
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: The Shine-Dalgarno hybrid during initiation of translation and elongation / D. Maraschio, Teana La, P. Volpe // Біополімери і клітина. — 2007. — Т. 23, № 3. — С. 243-249. — Бібліогр.: 18 назв. — англ.

It was unknown whether a synthetic Shine-Dalgarno (SD) oligonucleotide labelled with ³²P at its 5'-end ([³²P]oct) would be able to reach the anti-SD sequence of 16S rRNA at the early stages of translation only or during elongation. To verify this, [³²P]oct was incubated with 30S ribosomal subun...