PIP2;1 aquaporin gene expression in maize hybrids different for drought tolerance to water deficit
At the mRNA level, different PIP2;1 aquaporin gene expressions in roots of drought-tolerant and drought-sensitive maize genotypes with sufficient substrate moisture, as well as their distinct responses towards water deficit, are shown. An analysis of the obtained data allow us to consider PIP2;1 e...
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| Cite this: | PIP2;1 aquaporin gene expression in maize hybrids different for drought tolerance to water deficit / I.I. Ovrutska, E.L. Kordyum // Доповіді Національної академії наук України. — 2019. — № 5. — С. 97-101. — Бібліогр.: 15 назв. — англ. |
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Ovrutska, I.I. Kordyum, E.L. 2019-07-15T15:54:47Z 2019-07-15T15:54:47Z 2019 PIP2;1 aquaporin gene expression in maize hybrids different for drought tolerance to water deficit / I.I. Ovrutska, E.L. Kordyum // Доповіді Національної академії наук України. — 2019. — № 5. — С. 97-101. — Бібліогр.: 15 назв. — англ. 1025-6415 DOI: doi.org/10.15407/dopovidi2019.05.097 https://nasplib.isofts.kiev.ua/handle/123456789/158112 579.258:577.112:581.43:582.542.11:58.032.1 At the mRNA level, different PIP2;1 aquaporin gene expressions in roots of drought-tolerant and drought-sensitive maize genotypes with sufficient substrate moisture, as well as their distinct responses towards water deficit, are shown. An analysis of the obtained data allow us to consider PIP2;1 expression as a possible molecular marker in maize breeding for drought tolerance. На рівні mRNA показано відмінності в експресії гена PIP2;1 аквапорину в коренях стійких і чутливих до посухи гібридів кукурудзи при достатній вологості субстрату, а також різні реакції генотипів на водний дефіцит. На підставі одержаних даних експресію гена PIP2;1 можна розглядати як можливий молекулярний маркер в селекції кукурудзи на посухостійкість. На уровне mRNA показаны отличия в експрессии гена PIP2;1 аквапорина в корнях стойких и чувствительных к засухе гибридов кукурузы при достаточной влажности субстрата, а также различные реакции генотипов на водный дефицит. Анализ полученных данных позволяет рассматривать экспрессию гена PIP2;1 в качестве возможного молекулярного маркера в селекции кукурузы на засухоустойчивость. en Видавничий дім "Академперіодика" НАН України Доповіді НАН України Біологія PIP2;1 aquaporin gene expression in maize hybrids different for drought tolerance to water deficit Експресія гена аквапорину PIP2;1 у різних за стійкістю до посухи гібридів Zea mays l. Экспрессия гена аквапорина PIP2;1 у различных по стойкости к засухе гибридов Zеa mays l. Article published earlier |
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PIP2;1 aquaporin gene expression in maize hybrids different for drought tolerance to water deficit |
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PIP2;1 aquaporin gene expression in maize hybrids different for drought tolerance to water deficit Ovrutska, I.I. Kordyum, E.L. Біологія |
| title_short |
PIP2;1 aquaporin gene expression in maize hybrids different for drought tolerance to water deficit |
| title_full |
PIP2;1 aquaporin gene expression in maize hybrids different for drought tolerance to water deficit |
| title_fullStr |
PIP2;1 aquaporin gene expression in maize hybrids different for drought tolerance to water deficit |
| title_full_unstemmed |
PIP2;1 aquaporin gene expression in maize hybrids different for drought tolerance to water deficit |
| title_sort |
pip2;1 aquaporin gene expression in maize hybrids different for drought tolerance to water deficit |
| author |
Ovrutska, I.I. Kordyum, E.L. |
| author_facet |
Ovrutska, I.I. Kordyum, E.L. |
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Біологія |
| topic_facet |
Біологія |
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2019 |
| language |
English |
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Доповіді НАН України |
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Видавничий дім "Академперіодика" НАН України |
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Article |
| title_alt |
Експресія гена аквапорину PIP2;1 у різних за стійкістю до посухи гібридів Zea mays l. Экспрессия гена аквапорина PIP2;1 у различных по стойкости к засухе гибридов Zеa mays l. |
| description |
At the mRNA level, different PIP2;1 aquaporin gene expressions in roots of drought-tolerant and drought-sensitive
maize genotypes with sufficient substrate moisture, as well as their distinct responses towards water deficit, are
shown. An analysis of the obtained data allow us to consider PIP2;1 expression as a possible molecular marker in
maize breeding for drought tolerance.
На рівні mRNA показано відмінності в експресії гена PIP2;1 аквапорину в коренях стійких і чутливих до
посухи гібридів кукурудзи при достатній вологості субстрату, а також різні реакції генотипів на водний
дефіцит. На підставі одержаних даних експресію гена PIP2;1 можна розглядати як можливий молекулярний маркер в селекції кукурудзи на посухостійкість.
На уровне mRNA показаны отличия в експрессии гена PIP2;1 аквапорина в корнях стойких и чувствительных к засухе гибридов кукурузы при достаточной влажности субстрата, а также различные реакции генотипов на водный дефицит. Анализ полученных данных позволяет рассматривать экспрессию гена PIP2;1 в
качестве возможного молекулярного маркера в селекции кукурузы на засухоустойчивость.
|
| issn |
1025-6415 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/158112 |
| citation_txt |
PIP2;1 aquaporin gene expression in maize hybrids different for drought tolerance to water deficit / I.I. Ovrutska, E.L. Kordyum // Доповіді Національної академії наук України. — 2019. — № 5. — С. 97-101. — Бібліогр.: 15 назв. — англ. |
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AT ovrutskaii pip21aquaporingeneexpressioninmaizehybridsdifferentfordroughttolerancetowaterdeficit AT kordyumel pip21aquaporingeneexpressioninmaizehybridsdifferentfordroughttolerancetowaterdeficit AT ovrutskaii ekspresíâgenaakvaporinupip21uríznihzastíikístûdoposuhigíbridívzeamaysl AT kordyumel ekspresíâgenaakvaporinupip21uríznihzastíikístûdoposuhigíbridívzeamaysl AT ovrutskaii ékspressiâgenaakvaporinapip21urazličnyhpostoikostikzasuhegibridovzeamaysl AT kordyumel ékspressiâgenaakvaporinapip21urazličnyhpostoikostikzasuhegibridovzeamaysl |
| first_indexed |
2025-11-25T12:57:46Z |
| last_indexed |
2025-11-25T12:57:46Z |
| _version_ |
1850514985309962240 |
| fulltext |
97ISSN 1025-6415. Допов. Нац. акад. наук Укр. 2019. № 5
doi: https://doi.org/10.15407/dopovidi2019.05.097
UDC 579.258:577.112:581.43:582.542.11:58.032.1
I.I. Ovrutska, E.L. Kordyum
M.G. Kholodny Institute of Botany of the NAS of Ukraine, Kiev
E-mail: cellbiol@ukr.net, airin1900@gmail.com
PIP2;1 aquaporin gene expression in maize hybrids
different for drought tolerance to water deficit
Presented by Corresponding Member of the NAS of Ukraine E.L. Kordyum
At the mRNA level, different PIP2;1 aquaporin gene expressions in roots of drought-tolerant and drought-sensitive
maize genotypes with sufficient substrate moisture, as well as their distinct responses towards water deficit, are
shown. An analysis of the obtained data allow us to consider PIP2;1 expression as a possible molecular marker in
maize breeding for drought tolerance.
Keywords: Zea mays L., drought tolerance, PIP2;1-aquaporin expression, RT-PCR.
To date, it is well known that aquaporins, which are membrane proteins forming the water chan-
nels, ensure the transport of water through membranes and/or facilitate the transport of small
neutral solutes (urea, boric acid, silicic acid) or gases (ammonia, carbon dioxide), and thus they
are involved in many basic vital important processes such as cell signalling, stress responses and
nutrient acquisition [1–3]. The multiple mechanisms, including the regulation of transcript or
protein abundance, subcellular trafficking, or gating by phosphorylation or cytosolic protons, can
regulate the aquaporin transport activity [2].
Aquaporins of the plasmalemma (plasma membrane intrinsic proteins, PIPs) are the most
numerous family in plants, which is divided in 2 subfamilies: РІР1 and РІР2, the latter is charac-
terized with a significantly more capability of the water tranport in comparison with PIP1[4]. In
the Arabidopsis thaliana L. genome, there are 35 genes coding aquaproteins [5, 6], 36 genes — in
Zea mays L. [7], and 33 genes in Oryza sativa L. [8]. Different responses of PIP aquaporin genes
to water stress — enhancement or weakening of the expression or absence of any response — have
been established that supposes their unequal role in the regulation of the water transport through
a membrane. It is suggested that aquaporins play distinct roles in facilitating a water flux and
maintaining the water potential in cells along with various water transport activities. In addition,
aquaporins are involved in the plant adaptation to water deficiency [3, 9]. Therefore, an aim of our
work was to study the PIP2;1 aquaporin gene expression in four, different for drought resistant
hybrids of Z. mays grown under conditions of low soil moisture.
© I.I. Ovrutska, E.L. Kordyum, 2019
98 ISSN 1025-6415. Dopov. Nac. akad. nauk Ukr. 2019. № 5
I.I. Ovrutska, E.L. Kordyum
Material and Methods. Four corn hybrids were selected, namely Dostatok (drought re-
sistant) and Pereiaslavskii (moderately drought resistant) kindly provided by the Institute of
Physiology and Genetics of the National Academy of Sciences of Ukraine (Kyiv), and Flagman
(drought resistant) and Yacht (moderately drought resistant) kindly provided by the Breeding
and Genetic Institute – a National Center for Seed Research and Variety Research (Odessa). 120
plants were grown in vegetation vessels filled with sandy substrate of optimum 70 % moisture
under the tent during 10 days. Then 60 plants continued to grow under these conditions and were
kept as controls, 60 plants continued to grow in the substrate of 30 % moisture for 10 days as this
period of drought is critical for plant growth. Experiments were performed in three replicates.
Substrate moisture was controlled every two days according to [10, pp. 75-77].
The relative amounts of expression of mRNA encoding PIP2;1 aquaporin were determined
in corn roots by the reverse transcription-polymerase chain reaction (RT-PCR). Total RNA was
extracted using TRI-REAGENT (Sigma) according to the manufacturer protocol. Yield was de-
termined using the absorbance of RNA at 260 nm. RT-PCR was carried on the FERMENTAS
protocol (Lithuana) with an amplificator Terzik (DNA-technology, Russia). PCR was carried us-
ing the obtained cDNA and primers with 19-21 nucleotides:
Forward 5′- GTT CCA GAG CGC CTA CTT C -3′
Reverse 5′- GGG CTT GTC CTT GTT GTA GAT -3′.
The PCR products were separated on 1.5 % agarous gel, visualized in ultraviolet light and
photographed using the system of gel visualization Bio-Vision (VILBER LOURMAT). Actin
gene expression was used as an endogenic control on passing the reaction with primers of 18–20
nucleotides.
Forward 5′- GTT CCA ACC ATC CCT TGT-3′
Reverse 5′- CGT GAT CTC CTT GCT CAT AC-3′.
The level of gene expression was measured using GelAnalyzer2010a (www.GelAnalyzer.exe).
Software Image Mater Total Lab™ was used to estimate the quantity of products. The obtained
data are processed statistically using Microsoft Excel 2013.
Results and Discussion. At the mRNA level, different expressions of PIP2;1 in roots of
drought-tolerant and drought-sensitive genotypes in normal conditions (substrate moisture
70 %), as well as their distinct responses towards water deficit (substrate moisture 30 %) (Fig. 1,
2), are shown.
Expression of PIP2;1 increased in Dostatok and Flagman growing on the substrate with
30 % moisture, while expression of this gene lowered or remained at about the same level in
Pereiaslavskii and Yacht under these conditions. In addition, these data indicate up-regulation of
PIP2;1 transcripts in drought-resistance hybrid and down-regulation of transcripts in drought-
sensitive upon water deficit, which supposes the genotype-specific aquaporin transcription in in-
vestigated maize genotypes.
The obtained results are consistent with available literature data on the participation of PIP
aquaporins in plant responses to drought stress. So, distinct responses of PIP aquaporin genes
to water stress by treatment with 20 % PEG have been revealed in Oryza sativa L. cultivars:
drought-resistance upland Zhonghan 3 (spp. Indica) and drought-sensitive lowland Xiushui 63
(spp. Japonica) [11]. PIP aquaporin gene expression was significantly up-regulated in upland rice,
whereas it remained unchanged or down-regulated in lowland rice. Up- or down-regulation of
99ISSN 1025-6415. Допов. Нац. акад. наук Укр. 2019. № 5
PIP2;1 aquaporin gene expression in maize hybrids different for drought tolerance to water deficit
PIP genes was also described in A. thaliana under water deficit after the treatment with a 250-
mM mannitol solution [6, 7]. Aquaporin gene expression varied in a Vitis hybrid (V. berlandieri ×
V. rupestris) Richter-110 depending on the type of aquaporin and the water stress intensity [12].
The essential differences in РІР2 aquaporin gene expression has been revealed in closely re-
lated, but ecologically different Sium latifolium L. and Sium sisaroideum L. The level of mRNA
of РІР2 aquaporin was higher in S. sisaroideum terrestrial plants growing under the conditions
of moderate water deficit in comparison with that in aerial-aquatic S. latifolium growing in a
river near the bank, and it increased when the temperature rose to 35—40 °C [13]. PIP aquaporin
gene divergent expressions were shown in tolerant and sensitive genotypes of Saccharum offici-
narum L. under normal and drought stress conditions, pointing to the aquaporin transcription
m
R
N
A
, r
el
. u
n.
1.0
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
Soil moisture 70 %
Soil moisture 30 %
Pereiaslaskii Dostatok Yacht Flagman
Fig. 1. Agarous gel-electrophoresis with the products of RT–PCR, PIP2;1 gene. 1 –
Pereiaslavskii; 2 – Dostatok; 3 – Yacht; 4 – Flagman. М — marker, C — control (sub-
strate moisture 70 %), E — experiment (substrate moisture 30 %)
Fig. 2. PIP2;1 aquaporin gene expression in roots of maize hybrids different for drought
tolerance grown in substrates with 70 % and 30 % moisture
100 ISSN 1025-6415. Dopov. Nac. akad. nauk Ukr. 2019. № 5
I.I. Ovrutska, E.L. Kordyum
in this species to be potentially genotype-specific [14], that is especially important for scanning
molecular markers for plant breeding.
In our experiments, the genotype-specific regulation of PIP aquaporin gene expression was
also revealed in responses of investigated maize hybrids different for drought tolerance to water
deficiency. In addition, up-regulation of PIP2;1 aquaporin gene in maize drought-resistance hy-
brid Dostatok under 30 % substrate moisture correlated with the essential enhancement in the
H+-ATPase hydrolytic activity, which was higher about three times in comparison with that in
more drought-sensitive hybrid Pereiaslavskii [15]. The obtained data allow us to consider the
PIP2;1 aquaporin gene expression as a molecular marker in maize breeding for drought toler-
ance.
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arplant.59.032607.092734
3. Li, G., Santoni, V. & Maurel, C. (2014). Plant aquaporins: roles in plant physiology. Biochim. Biophys.
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5. Jang, J. Y., Kim, D. G., Kim, Y. O., Kim, J. S. & Kang, H. (2004). An expression analysis of a gene family encod-
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highly divergent protein family in maize. Plant Physiol., 125, pp. 1206-1215. PMCID: PMC65601
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9. Kapilan, R., Vaziri, M. & Zwiazek, J. J.(2018). Regulation of aquaporins in plants under stress. Biol Res., 51,
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10. Voizechivska, O. V., Kapustian, A. V., Kosyk, O. I., Musienko, M. M., Olchovich, O. P., Paniuta, O. O., Parshi-
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101ISSN 1025-6415. Допов. Нац. акад. наук Укр. 2019. № 5
PIP2;1 aquaporin gene expression in maize hybrids different for drought tolerance to water deficit
15. Ovrutska, I. I. & Bluma, D. A. (2016). Water deficit induces changes in H+-ATPase activity and its gene
expression in Zea mays L. roots. Dopov. Nac. acad. nauk Ukr., 10, pp. 84-87. doi: https://doi.org/10.15407/
dopovidi2016.10.084
Received 28.02.2019
I.I. Овруцька, Є.Л. Кордюм
Інститут ботаніки ім. М.Г. Холодного НАН України, Київ
E-mail: cellbiol@ukr.net, airin1900@gmail.com
ЕКСПРЕСІЯ ГЕНА АКВАПОРИНУ PIP2;1 У РІЗНИХ
ЗА СТІЙКІСТЮ ДО ПОСУХИ ГІБРИДІВ ZEA MAYS L.
На рівні mRNA показано відмінності в експресії гена PIP2;1 аквапорину в коренях стійких і чутливих до
посухи гібридів кукурудзи при достатній вологості субстрату, а також різні реакції генотипів на водний
дефіцит. На підставі одержаних даних експресію гена PIP2;1 можна розглядати як можливий молекуляр-
ний маркер в селекції кукурудзи на посухостійкість.
Ключові слова: Zea mays L., посухостійкість, експресія гена PIP2;1-аквапорин, ПЛР зі зворотною тран-
скрипцією.
И.И. Овруцкая, Е.Л. Кордюм
Институт ботаники им. Н.Г. Холодного НАН Украины, Киев
E-mail: cellbiol@ukr.net, airin1900@gmail.com
ЭКСПРЕССИЯ ГЕНА АКВАПОРИНА PIP2;1 У РАЗЛИЧНЫХ
ПО СТОЙКОСТИ К ЗАСУХЕ ГИБРИДОВ ZЕA MAYS L.
На уровне mRNA показаны отличия в експрессии гена PIP2;1 аквапорина в корнях стойких и чувствитель-
ных к засухе гибридов кукурузы при достаточной влажности субстрата, а также различные реакции гено-
типов на водный дефицит. Анализ полученных данных позволяет рассматривать экспрессию гена PIP2;1 в
качестве возможного молекулярного маркера в селекции кукурузы на засухоустойчивость.
Ключевые слова: Zea mays L., засухоустойчивость, экспрессия гена PIP2;1-аквапорин, ПЦР с обратной
транскрипцией.
|