Rhizopus sp. 2000 FM – the active fungi exolipase producer
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Інститут молекулярної біології і генетики НАН України
2009
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| Zitieren: | Rhizopus sp. 2000 FM – the active fungi exolipase producer / A.V. Borisenko, M.N. Antonuk, V.L. Aisenberg, A.P. Kapichon, V.A. Stoyko // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2009. — Т. 6. — С. 202-204. — Бібліогр.: 9 назв. — eng. |
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Borisenko, A.V. Antonuk, M.N. Aisenberg, V.L. Kapichon, A.P. Stoyko, V.A. 2021-02-02T21:18:15Z 2021-02-02T21:18:15Z 2009 Rhizopus sp. 2000 FM – the active fungi exolipase producer / A.V. Borisenko, M.N. Antonuk, V.L. Aisenberg, A.P. Kapichon, V.A. Stoyko // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2009. — Т. 6. — С. 202-204. — Бібліогр.: 9 назв. — eng. 2219-3782 https://nasplib.isofts.kiev.ua/handle/123456789/175923 en Інститут молекулярної біології і генетики НАН України Фактори експериментальної еволюції організмів Аналіз і оцінка генетичних ресурсів Rhizopus sp. 2000 FM – the active fungi exolipase producer Article published earlier |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine |
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DSpace DC |
| title |
Rhizopus sp. 2000 FM – the active fungi exolipase producer |
| spellingShingle |
Rhizopus sp. 2000 FM – the active fungi exolipase producer Borisenko, A.V. Antonuk, M.N. Aisenberg, V.L. Kapichon, A.P. Stoyko, V.A. Аналіз і оцінка генетичних ресурсів |
| title_short |
Rhizopus sp. 2000 FM – the active fungi exolipase producer |
| title_full |
Rhizopus sp. 2000 FM – the active fungi exolipase producer |
| title_fullStr |
Rhizopus sp. 2000 FM – the active fungi exolipase producer |
| title_full_unstemmed |
Rhizopus sp. 2000 FM – the active fungi exolipase producer |
| title_sort |
rhizopus sp. 2000 fm – the active fungi exolipase producer |
| author |
Borisenko, A.V. Antonuk, M.N. Aisenberg, V.L. Kapichon, A.P. Stoyko, V.A. |
| author_facet |
Borisenko, A.V. Antonuk, M.N. Aisenberg, V.L. Kapichon, A.P. Stoyko, V.A. |
| topic |
Аналіз і оцінка генетичних ресурсів |
| topic_facet |
Аналіз і оцінка генетичних ресурсів |
| publishDate |
2009 |
| language |
English |
| container_title |
Фактори експериментальної еволюції організмів |
| publisher |
Інститут молекулярної біології і генетики НАН України |
| format |
Article |
| issn |
2219-3782 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/175923 |
| citation_txt |
Rhizopus sp. 2000 FM – the active fungi exolipase producer / A.V. Borisenko, M.N. Antonuk, V.L. Aisenberg, A.P. Kapichon, V.A. Stoyko // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2009. — Т. 6. — С. 202-204. — Бібліогр.: 9 назв. — eng. |
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| first_indexed |
2025-11-24T15:54:02Z |
| last_indexed |
2025-11-24T15:54:02Z |
| _version_ |
1850849152670367744 |
| fulltext |
202
интенсивность изменчивости в поколении М2 могут быть показателями генетической
нестабильности организмов.
It is established, that action of low doses γ-rays causes in the second generation high
frequency of the changed signs in winter wheat plants. The minimum dose 0,5 Гр has appeared
genetically highly effective. At low doses of irradiation frequency and intensity of variability in
generation М2 can be indicators of genetic instability of organisms.
1BORISENKO A.V., 1ANTONUK M.N., 2AISENBERG V.L., 2KAPICHON A.P.,
2 STOYKO V.A.
1National University of Food Technology vul. Volodymyrska, 68, Kyiv, 01033. Ukraine
2D.K.Zabolotny Institute of Microbiology and Virology, Ukrainian National Academy of Sciences,
vul. Acad-Zabolotny, 154, Kyiv, 03680. Ukraine
RHIZOPUS sp. 2000 FM – THE ACTIVE FUNGI EXOLIPASE PRODUCER
Some physiological-biochemical properties of the new fungus strain Rhizopus sp. 2000 FM
being active source of exolipase. The highest levels of lipolytic activity (LA) were obtained at the
fungus cultivation at the substrate with: sun-flower oil, sucrose and with starch. The LA evaluation
was performed with the use of the spectrophotometric method and n-nitrophenyl-palmitate as a
chromogenic substrate. This method was approved experimentally in the Department of
Physiology and Systematic of Micromycetes, D.K.Zabolotny Institute of Microbiology and
Virology National Academy of Sciences of Ukraine (NASU)/ We have not established the direct
correlation between the level of biomass accumulation and LA level. The data obtained on the
studies of carbon nutrition on the growth and LA of the new perspective source of lipase allows to
optimize its nutrition substrate in future. Selection work on the fungi cultures creation and
modification to use them as the sources of the ferments with the new properties is one of the
most significant biotechnology direction. Among the variety of the known ferments only few have
the same perspectives as the lipases do.
The lipolytic ferments could be determined as he hydrolases of the fat acids' esters with the
long chain. The substrates for the lipases action are the lipids. According to the Ferments
Nomenclature, the lipase has the name of triacylglycerolhydralase (KF 3.1.1.3). Lipases are the
ferments of the surface action and activize being localized on the surface of the sub -strate non-
dissolvable in water [1]. Being the natural, fats' splitters, the lipolytic ferments are very interesting
for those branches of industry, where the total or partial hydrolysis of fats and oils is needed and
for the medical and industrial branches of application [2]. Lipases are widely used in food industry
(cheese production and non-alcoholic drinks); in confectionery for chocolate and caramel
production: in flour-milling and bakery for bread quality amelioration and its storage term
prolongation. Lipases will find application in the technology creation of essential fat acids for
food and drugs production [2]. Lipolytic ferments could be used as well for esterification and re-
estenfication of fat acids in glycerins, what, in turn, opens wider possibilities for creation of the
fat products with needed functional properties. New technologies with the use of immobilized
microbial lipases are being introduced. Lipases are actively used in medicine as a therapeutic means
for the gastroenterological diseases and in medical diagnostics [4].
The lipases' significance is high both in cosmetics, in fur and skin industries for bettering the
elasticity of the products and excellent natural appearance, in natural silk production for fat removal
through its hydrolysis with the use of lipolytic fermentative preparations. The need in thermophilic
lipases' sources of microbial origin is high in textile industry as well where the wax type substances
are removed by lipases under the temperatures in the range from 40 ºС to 60 °C. The introduction of
the thermostable lipases into national industry of washing products is not less perspective for the
fats removal from waste waters, especially for the canalization communications and wastes
203
processing. Lipases could be successfully used in agriculture for the development of animal food
preparation with the scope of metabolism bettering [1, 3, 5].
Nowadays, the lipolytic ferments production in Ukraine is not organized and, thus, the
creation of the competitive national technology of these compounds is an actual. The determining
and limiting factor in the lipolytic ferments' production is the lack of the stable and productive
microorganisms - the producents, which could be introduced into technological circle effectively.
The fungi, in difference from bacteria, which accumulate mainly the inner cell lipase produce
mainly the out-cell ferment [6].
The significant input to the experimental mycology was made by the staff members of the
Department of Physiology and Systematic of Micromycetes, D.K.Zabolomy Institute of
Microbiology and Virology, NASU, (DPSM-Zabolotny-NASU) who perform the research on the
extraction and identification of fungi isolates from various substrates [7]. One of the main directions
of the biologically active fungal metabolites studies is the estimation of the conditions of their
effective biosynthesis and demonstration their properties.
The scope of the work was the research of physiologyical and biochemical properties of the
new active producer of out-cell lipase - Rhizopus sp. 2000 FM, especially, of the influence of the
carbon nutrition sources on the activity of the fungi produced ferment.
Materials and methods
The object of the research was the thermotolerant fungus Rhizopus sp. 2000 FM. The deep
cultivation was performed on the nutritive substrate under investigation during 72 hours at the
temperature of 39 – 40 °C. For Rhizopus sp. 2000 FM cultivation the nutritive substrates with
Chapek ambient were used with the addition of different carbon sources in various concentrations.
The influence of the carbon nutrition components on LA and fungus Rhizopus sp. 2000 FM growth
was studied experimentally through the change of carbon sources in equivalent relation. The
following plants oils were used as the carbon sources: sunflower, olive, lemon, castor, raps, walnut,
maize; carbohydrates (glucose, arabinose, maltose, xylose, sucrose, lactose, starch); polyatomic
alcohols (inosit, sorbit, doulcit) and surface-active compound (SAC) - twin-20.
One of the causes met by researchers studying the microorganisms' ability to exolipase
synthesis is і he absence of the express and correct method of LA evaluation. Spectrophotometric
method (SPM) used in the present work for LA evaluation was approved at the DPSM-Zabolotny-
NASU.
The reasoning of the SPM: the micromycetes cultural liquid's lipase action on chromogenic
substrate -phenolic ester of palmitate acid with the release of n-nitrophenole (n-NP) as a result of
reaction what. :n turn, stimulates the changes of optical density of the reaction mixture what is
registered by spec-trophotometer at the wavelength of 410 nm. As the unit of LA was taken such
quantity of the ferment in 1 ml which catalizes the freeing of 1 nM of n-NP from the emulgated
substrate during 1 minute at 3 ºC. The level of the biomass accumulation in the cultural ambient
was detected by weighting method [9].
Results and discussion
During research, the intensive growth of aerobe culture was detected on mash agare. It is
isolated from the soil specimen. The strain creates white colonies with light yellow coloring. The
fungus is the thermotolerant one with temperature range of the growth from 19 ºС to 50 °C.
Temperature optimum of the growth is 36-39ºC speculation 40-42 °C
The results obtained during the cultivation of the fungus Rhizopus sp. 2000 FM on the
nutritive substrate with different oils as the carbon sources in various concentrations have shown
that the highest level of LA for the fungus is achieved on the substrate with the sunflower oil (1 %).
It was shown that the presence of the oils: sunflower, castor, walnut taken in concentration induces
the exolipase synthesis by fungus Rhizopus sp. 2000 FM.
Substantial LA was detected on the substrates with the use of sucrose and starch. During the
Rhizopus sp. 2000 FM cultivation on the substrates with polyatomic alcohols and with twin-20 as
the carbon sources, the low growth and the LA absence were registered. The fungus Rhizopus sp.
2000 FM strain under the cultivation on the studied sources has demonstrated moderate growth rate.
204
The greatest biomass accumulation was registered during the fungus cultivation on the substrate
with the addition of 1% of walnut oil at the comparatively low LA. The direct correlation between
the biomass accumulation and. its LA was not established. The culture growth on the substrate with
the addition of sunflower oil was accompanied by medium oxidation from 7.0 (pH of the medium
before cultivation) to 5.0.
The experimental results support the fact that the sunflower oil is the effective inductor of
exolipase biosynthesis. The high values of LA demonstrated on the medium with sucrose and starch
addition are exponents of the physiological and biochemical properties of the strain Rhizopus sp.
2000 FM.
Maximal activity is registered for the substrate with addition of the 1% sunflower oil.
Further increase of this oil caused the decrease of the fermentative activity, thus, one could consider
the sunflower oil to be not only the inductor but also the represser of the lipase biosynthesis. What
concerns the growth and biomass accumulation we could state that the addition of the significant
quantity of the sunflower oil, stimulates the growth of the micromycete Rhizopus sp. 2000 FM.
The data on the growth rate and LA of the fungus strain Rhizopus sp. 2000 ФМ on the
carbon sources, along with the detection of other components of the medium, is believed to support
the optimization the content of the nutritive medium for the cultivation of the new active exolipase
producent – fungus Rhizopus sp. 2000 FM.
Literature
1. Gracbeva I.M. Technology of Fermentative Preparations (in Russian) - 3rd Edition. Moscow:
ELEVAR Publishing, 2000.–512 pp.
2. Brockerhof H., Jensen R. Lipolytic Ferments - Moscow: MIR Publishers, 1978.- 388 pp.
3. Yakovenko E.P. Fermentative Preparations in Clinical Practice (in Russian) // Clinical
Pharmacology and Therapy.– 2004.-N1– p. 17-20.
4. Pyrog T.P. General Microbiology (in Ukrainian): Manual. -Kyiv. National University of
Chemical Technology, 2004.–р.187-204.
5. Bilay VI. Fundamentals of General Mycology (in Russian) 2nd Edition. - Kyiv: Vyshcha Shkola
Publishers, 1980. -360 pp.
6. Zbdanova N.N. Development of the Research in the Field of Experimental Mycology (in
Russian)//Journal of Microbiology (Russia) – 2002 – v.60. N 5. – p.48 - 54.
7. Bekker Z.E. Physiology and Biochemistry of Fungi. -Moscow: MIR Publishers 1988. – 230 pp.
8. Aisenberg V.L., Karpel V.I., Svrchm S.A., Kapicbon A.P. Quantitative Met hod of Lipolvtic
Actiritv Evaluation Approbation with the Use of Cbromogenic Substrate (in Russian)//Journal of
Microbiolog (Russia). - 1995- -v.5 -N5 - p.84-89.
9. Bilay Y.l. Methods of Experimental Mycology (in Russian). Data Book - Kyiv: Naukova Dumka
Publishers. 1982. -550 pp.
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