Understanding the mechanism of DNA threshold elongation
The mechanism of threshold elongation of DNA macromolecule (overstretching) is studied within the frame-work of phenomenological approach, accounting both external (stretching) and internal (conformational) dis-placement components. As shown, the overstretching of DNA under the action of an external...
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| Veröffentlicht in: | Физика низких температур |
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| Datum: | 2018 |
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| Sprache: | English |
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Фізико-технічний інститут низьких температур ім. Б.І. Вєркіна НАН України
2018
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| Zitieren: | Understanding the mechanism of DNA threshold elongation / S.N. Volkov // Физика низких температур. — 2018. — Т. 44, № 7. — С. 893-904. — Бібліогр.: 64 назв. — англ. |
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Volkov, S.N. 2021-02-04T07:10:03Z 2021-02-04T07:10:03Z 2018 Understanding the mechanism of DNA threshold elongation / S.N. Volkov // Физика низких температур. — 2018. — Т. 44, № 7. — С. 893-904. — Бібліогр.: 64 назв. — англ. 0132-6414 PACS: 87.14.gk, 87.15.La, 87.15.hp, 63.20.Pw https://nasplib.isofts.kiev.ua/handle/123456789/176203 The mechanism of threshold elongation of DNA macromolecule (overstretching) is studied within the frame-work of phenomenological approach, accounting both external (stretching) and internal (conformational) dis-placement components. As shown, the overstretching of DNA under the action of an external force can occur in two stages. Firstly, due to the coupling between the components, at a some critical value of external force a conformational bistability is formed in the macromolecule structure. In turn, the appearance of bistability stimulates the formation of domains in the DNA chain with two different conformations (B and S). Secondly, under favorable boundary conditions, the conformationally induced deformation acquires the possibility to propagate along the macromolecule as domain walls. In this way the bistability occurrence in the macromolecule conformation provides a threshold effect of elongation. The calculated contributions in DNA overstretching show agreement with the observed data, and allow to explain the dependence of macromolecule threshold elongation on nucleotide content. I am grateful to Arnold Markovich Kosevich for fruitful cooperation and stimulating discussions for a number of years. I also would like to thank Maxim Frank-Kamenetskii for critical remarks and useful discussion of the presented results. As well, I want to thank Richard Lavery for sending the data on conformational analysis of a stretched DNA, and Polina Kanevska for help in making drawings and discussion. The present work was partially supported by the Program of Fundamental Research of the Department of Physics and Astronomy of the National Academy of Sciences of Ukraine. en Фізико-технічний інститут низьких температур ім. Б.І. Вєркіна НАН України Физика низких температур Свойства нелинейных биофизических систем со сложной внутренней структурой Understanding the mechanism of DNA threshold elongation Article published earlier |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| title |
Understanding the mechanism of DNA threshold elongation |
| spellingShingle |
Understanding the mechanism of DNA threshold elongation Volkov, S.N. Свойства нелинейных биофизических систем со сложной внутренней структурой |
| title_short |
Understanding the mechanism of DNA threshold elongation |
| title_full |
Understanding the mechanism of DNA threshold elongation |
| title_fullStr |
Understanding the mechanism of DNA threshold elongation |
| title_full_unstemmed |
Understanding the mechanism of DNA threshold elongation |
| title_sort |
understanding the mechanism of dna threshold elongation |
| author |
Volkov, S.N. |
| author_facet |
Volkov, S.N. |
| topic |
Свойства нелинейных биофизических систем со сложной внутренней структурой |
| topic_facet |
Свойства нелинейных биофизических систем со сложной внутренней структурой |
| publishDate |
2018 |
| language |
English |
| container_title |
Физика низких температур |
| publisher |
Фізико-технічний інститут низьких температур ім. Б.І. Вєркіна НАН України |
| format |
Article |
| description |
The mechanism of threshold elongation of DNA macromolecule (overstretching) is studied within the frame-work of phenomenological approach, accounting both external (stretching) and internal (conformational) dis-placement components. As shown, the overstretching of DNA under the action of an external force can occur in two stages. Firstly, due to the coupling between the components, at a some critical value of external force a conformational bistability is formed in the macromolecule structure. In turn, the appearance of bistability stimulates the formation of domains in the DNA chain with two different conformations (B and S). Secondly, under favorable boundary conditions, the conformationally induced deformation acquires the possibility to propagate along the macromolecule as domain walls. In this way the bistability occurrence in the macromolecule conformation provides a threshold effect of elongation. The calculated contributions in DNA overstretching show agreement with the observed data, and allow to explain the dependence of macromolecule threshold elongation on nucleotide content.
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| issn |
0132-6414 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/176203 |
| fulltext |
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| citation_txt |
Understanding the mechanism of DNA threshold elongation / S.N. Volkov // Физика низких температур. — 2018. — Т. 44, № 7. — С. 893-904. — Бібліогр.: 64 назв. — англ. |
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AT volkovsn understandingthemechanismofdnathresholdelongation |
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2025-11-24T15:05:27Z |
| last_indexed |
2025-11-24T15:05:27Z |
| _version_ |
1850847462598639616 |