Биотехнологические подходы к улучшению хозяйственно-ценных признаков тритикале

• Published in: Фактори експериментальної еволюції організмів
• Date: 2015
• Main Authors: Дубовец, Н.И., Сычева, Е.А., Бондаревич, Е.Б., Соловей, Л.А.
• Format: Article
• Language: Russian
• Published: Інститут молекулярної біології і генетики НАН України 2015
• Subjects: Прикладна генетика і селекція
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/177351
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Биотехнологические подходы к улучшению хозяйственно-ценных признаков тритикале / Н.И. Дубовец, Е.А. Сычева, Е.Б. Бондаревич, Л.А. Соловей // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 16. — С. 105-109. — Бібліогр.: 6 назв. — рос.

Aims. Development of triticale secondary recombinant gene pool for use in breeding for semidwarfness and resistance
 to pre-harvest sprouting. Methods. Chromosome-engineering technologies in combination with molecular-cytogenetic
 and DNA labelling of genotypes. Results. Hybridization of substituted 6x-triticale forms with modern crop cultivars
 was carried out. Eight stable recombinant lines with different D(A)- and D(B)-chromosome sunstitutions were selected
 out of hybrid populations F4
 by chromosome differential Giemsa staining (C-banding). Allelic composition of genes
 affecting formation of semidwarfness (Rht-B1, Rht8) and resistance to pre-harvest sprouting (Vp-1B) was investigated in
 the selected forms. Conclusions. A high frequency of D-genome chromosome inclusion in karyotype of triticale cultivars
 and dependence of the effectiveness of the introgression process on the genotypic environment of the developed hybrid
 form were revealed. Recombinant triticale lines, promising for breeding, were selected according to the data of moleculargenetic analysis.
 Keywords: triticale, chromosome-substitution lines, semidwarfness, resistance to pre-harvest sprouting, molecular
 markers.