Видовой состав и генетическая вариабельность бактериальных симбионтов тлей фауны Беларуси

• Published in: Фактори експериментальної еволюції організмів
• Date: 2015
• Main Authors: Воронова, Н.В., Сироткина, Д.П.
• Format: Article
• Language: Russian
• Published: Інститут молекулярної біології і генетики НАН України 2015
• Subjects: Еволюція геномів у природі та експерименті
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/177356
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Видовой состав и генетическая вариабельность бактериальных симбионтов тлей фауны Беларуси / Н.В. Воронова, Д.П. Сироткина // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 16. — С. 32-36. — Бібліогр.: 3 назв. — рос.

Aims. Symbiotic bacteria are of interest to biotechnology as potential tool for pest management. The aim of the study
 was to research the occurrence and diversity of secondary symbionts of specifi c genera in different species of aphids
 from Belarus. Methods. Biodiversity of bacterial symbionts was studied by PCR with genus-specifi ed primers. Genetic
 diversity was revealed with methods of bioinformatics. Results. The research showed that aphids of Belarusian fauna
 possess a wide range of symbiotic microorganisms. Among secondary symbionts of Belarusian aphids Rickettsia and
 Spiroplasma have almost 100 % frequency of occurrence despite the fact in literature they are considered as rare. Degree
 of the phylogenetic homogeneity of 16S rRNA and gltA genes in symbiotic rickettsia has been studied. It is shown that
 both the mean and the maximum interspecifi c genetic distances of 16S rRNA gene in pathogenic rickettsia have been
 signifi cantly lower than in symbiotic ones. Phylogenetic analysis of symbiotic rickettsia, based on 16S rRNA and gltA
 genes, has shown the existence of 4 separate clusters with high bootstrap value. Conclusions. The obtained data show
 possible existence of at least four different species of symbiotic bacteria in Rickettsia genus.
 Keywords: aphids, symbiotic bacteria, Rickettsia, PCR markers.