Гомологічна рекомбінація у гетерозиготних за мейотичними мутаціями рослин томату

• Published in: Фактори експериментальної еволюції організмів
• Date: 2015
• Main Author: Лісовська, Т.П.
• Format: Article
• Language: Ukrainian
• Published: Інститут молекулярної біології і генетики НАН України 2015
• Subjects: Еволюція геномів у природі та експерименті
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/177363
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Гомологічна рекомбінація у гетерозиготних за мейотичними мутаціями рослин томату / Т.П. Лісовська // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 16. — С. 58-62. — Бібліогр.: 17 назв. — укр.

Signifi cant progress in understanding the genetic control of meiosis and crossing-over, in particular, was obtained
 through research eukaryotic mutant organisms that violate the normal course of meiosis, called meiotic mutants. The 
 article presents the results of estimating the frequency of crossing-over in four heterozygotes for meiotic mutations
 tomato. Methods. The infl uence of mei-genes on recombination frequency was performed using lines that contain marker
 genes in the 2nd (wv, aw, d), 4th (e, ful), 6th (m-2, c) and 11 (hl, a) chromosomes. Sex difference in rf was determined
 on the basis of splitting of marker signs in populations F2
 and testcrosses of Fl
 as maternal form with multiple-marker
 tester. Expected value of rf the maximum likelihood method. Results. Mutations in the heterozygous state did not affect
 signifi cantly the frequency of crossing-over in marked areas of the genome according to F2, except heterozygotes As1/
 as1, which revealed rf reduction in area wv-aw. Assessment crossover frequency separately in female and male meiosis
 showed that in control — the initial variety Gloria and heterozygote Dsm1/dsm1 crossover frequency is higher in male
 meiosis (microsporogenesis) in heterozygotes Sti/sti rf frequency was signifi cantly higher in female meiosis. Conclusions.
 The meiotic mutation as1 and stі is recessive completely by cytological manifestation, but not completely recessive to
 infl uence the homologous recombination.
 Keywords: meiotic mutants, heterozygotes, crossing over, sex difference, tomato.