Introgressive wheat lines Triticum Aestivum/Amblyopyrum muticum as to structure of homoeologous group 5 chromosomes

• Veröffentlicht in: Фактори експериментальної еволюції організмів
• Datum: 2015
• Hauptverfasser: Iefimenko, T.S., Fedak, G., Antonyuk, M.Z., Ternovska, T.K.
• Format: Artikel
• Sprache: Englisch
• Veröffentlicht: Інститут молекулярної біології і генетики НАН України 2015
• Schlagworte: Еволюція геномів у природі та експерименті
• Online Zugang: https://nasplib.isofts.kiev.ua/handle/123456789/177367
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Zitieren: Introgressive wheat lines Triticum Aestivum/Amblyopyrum muticum as to structure of homoeologous group 5 chromosomes / T.S. Iefimenko, G. Fedak, M.Z. Antonyuk, T.K. Ternovska // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 16. — С. 83-86. — Бібліогр.: 14 назв. — англ.

Aims. Using SSR loci specifi c to homeological group 5 chromosomes, identify T genome introgressions in the genomes of
 F5
 wheat plants from crossing Aurora x Aurotica. Methods. DNA extraction using CTAB method, PCR with primers to SSR
 loci, electrophoresis in PAAG, silver staining. Results. Comparative microsatellite analysis of Aurora and Aurotica identifi ed
 9 SSR loci specifi c to D genome which produced differing amplicons with Aurotica DNA. These loci were used for screening (Aurora x Aurotica) F5
 progeny (234 plants); 24 plants with disomic substitutions 5D/5T were detected, and a signifi cant
 number of plants containing recombinant chromosome 5D/5T. The recombination event could occur in any generation from
 F2
 . Conclusions. Microsatellite loci could be used for identifi cation of introgressions from Aegilops mutica in wheat genome
 (although with some limitations). Identifi ed plants with recombinant 5D/5Т chromosomes are perspective for future work.
 Keywords: introgressive hybridization, Amblyopyrum muticum, microsatellite analysis, SSR loci, alien-substitution lines,
 recombinant lines.