Introgressive wheat lines Triticum Aestivum/Amblyopyrum muticum as to structure of homoeologous group 5 chromosomes

• Опубліковано в: : Фактори експериментальної еволюції організмів
• Дата: 2015
• Автори: Iefimenko, T.S., Fedak, G., Antonyuk, M.Z., Ternovska, T.K.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2015
• Теми: Еволюція геномів у природі та експерименті
• Онлайн доступ: https://nasplib.isofts.kiev.ua/handle/123456789/177367
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Introgressive wheat lines Triticum Aestivum/Amblyopyrum muticum as to structure of homoeologous group 5 chromosomes / T.S. Iefimenko, G. Fedak, M.Z. Antonyuk, T.K. Ternovska // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 16. — С. 83-86. — Бібліогр.: 14 назв. — англ.

Aims. Using SSR loci specifi c to homeological group 5 chromosomes, identify T genome introgressions in the genomes of F5 wheat plants from crossing Aurora x Aurotica. Methods. DNA extraction using CTAB method, PCR with primers to SSR loci, electrophoresis in PAAG, silver staining. Results. Comparative microsatellite analysis of Aurora and Aurotica identifi ed 9 SSR loci specifi c to D genome which produced differing amplicons with Aurotica DNA. These loci were used for screening (Aurora x Aurotica) F5 progeny (234 plants); 24 plants with disomic substitutions 5D/5T were detected, and a signifi cant number of plants containing recombinant chromosome 5D/5T. The recombination event could occur in any generation from F2 . Conclusions. Microsatellite loci could be used for identifi cation of introgressions from Aegilops mutica in wheat genome (although with some limitations). Identifi ed plants with recombinant 5D/5Т chromosomes are perspective for future work. Keywords: introgressive hybridization, Amblyopyrum muticum, microsatellite analysis, SSR loci, alien-substitution lines, recombinant lines.