Introgressive wheat lines Triticum Aestivum/Amblyopyrum muticum as to structure of homoeologous group 5 chromosomes
Aims. Using SSR loci specifi c to homeological group 5 chromosomes, identify T genome introgressions in the genomes of F5 wheat plants from crossing Aurora x Aurotica. Methods. DNA extraction using CTAB method, PCR with primers to SSR loci, electrophoresis in PAAG, silver staining. Results. Comp...
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Інститут молекулярної біології і генетики НАН України
2015
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| Zitieren: | Introgressive wheat lines Triticum Aestivum/Amblyopyrum muticum as to structure of homoeologous group 5 chromosomes / T.S. Iefimenko, G. Fedak, M.Z. Antonyuk, T.K. Ternovska // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 16. — С. 83-86. — Бібліогр.: 14 назв. — англ. |
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Iefimenko, T.S. Fedak, G. Antonyuk, M.Z. Ternovska, T.K. 2021-02-15T13:36:01Z 2021-02-15T13:36:01Z 2015 Introgressive wheat lines Triticum Aestivum/Amblyopyrum muticum as to structure of homoeologous group 5 chromosomes / T.S. Iefimenko, G. Fedak, M.Z. Antonyuk, T.K. Ternovska // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 16. — С. 83-86. — Бібліогр.: 14 назв. — англ. 2219-3782 https://nasplib.isofts.kiev.ua/handle/123456789/177367 631.523:581.13 Aims. Using SSR loci specifi c to homeological group 5 chromosomes, identify T genome introgressions in the genomes of F5 wheat plants from crossing Aurora x Aurotica. Methods. DNA extraction using CTAB method, PCR with primers to SSR loci, electrophoresis in PAAG, silver staining. Results. Comparative microsatellite analysis of Aurora and Aurotica identifi ed 9 SSR loci specifi c to D genome which produced differing amplicons with Aurotica DNA. These loci were used for screening (Aurora x Aurotica) F5 progeny (234 plants); 24 plants with disomic substitutions 5D/5T were detected, and a signifi cant number of plants containing recombinant chromosome 5D/5T. The recombination event could occur in any generation from F2 . Conclusions. Microsatellite loci could be used for identifi cation of introgressions from Aegilops mutica in wheat genome (although with some limitations). Identifi ed plants with recombinant 5D/5Т chromosomes are perspective for future work. Keywords: introgressive hybridization, Amblyopyrum muticum, microsatellite analysis, SSR loci, alien-substitution lines, recombinant lines. en Інститут молекулярної біології і генетики НАН України Фактори експериментальної еволюції організмів Еволюція геномів у природі та експерименті Introgressive wheat lines Triticum Aestivum/Amblyopyrum muticum as to structure of homoeologous group 5 chromosomes Article published earlier |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| title |
Introgressive wheat lines Triticum Aestivum/Amblyopyrum muticum as to structure of homoeologous group 5 chromosomes |
| spellingShingle |
Introgressive wheat lines Triticum Aestivum/Amblyopyrum muticum as to structure of homoeologous group 5 chromosomes Iefimenko, T.S. Fedak, G. Antonyuk, M.Z. Ternovska, T.K. Еволюція геномів у природі та експерименті |
| title_short |
Introgressive wheat lines Triticum Aestivum/Amblyopyrum muticum as to structure of homoeologous group 5 chromosomes |
| title_full |
Introgressive wheat lines Triticum Aestivum/Amblyopyrum muticum as to structure of homoeologous group 5 chromosomes |
| title_fullStr |
Introgressive wheat lines Triticum Aestivum/Amblyopyrum muticum as to structure of homoeologous group 5 chromosomes |
| title_full_unstemmed |
Introgressive wheat lines Triticum Aestivum/Amblyopyrum muticum as to structure of homoeologous group 5 chromosomes |
| title_sort |
introgressive wheat lines triticum aestivum/amblyopyrum muticum as to structure of homoeologous group 5 chromosomes |
| author |
Iefimenko, T.S. Fedak, G. Antonyuk, M.Z. Ternovska, T.K. |
| author_facet |
Iefimenko, T.S. Fedak, G. Antonyuk, M.Z. Ternovska, T.K. |
| topic |
Еволюція геномів у природі та експерименті |
| topic_facet |
Еволюція геномів у природі та експерименті |
| publishDate |
2015 |
| language |
English |
| container_title |
Фактори експериментальної еволюції організмів |
| publisher |
Інститут молекулярної біології і генетики НАН України |
| format |
Article |
| description |
Aims. Using SSR loci specifi c to homeological group 5 chromosomes, identify T genome introgressions in the genomes of
F5
wheat plants from crossing Aurora x Aurotica. Methods. DNA extraction using CTAB method, PCR with primers to SSR
loci, electrophoresis in PAAG, silver staining. Results. Comparative microsatellite analysis of Aurora and Aurotica identifi ed
9 SSR loci specifi c to D genome which produced differing amplicons with Aurotica DNA. These loci were used for screening (Aurora x Aurotica) F5
progeny (234 plants); 24 plants with disomic substitutions 5D/5T were detected, and a signifi cant
number of plants containing recombinant chromosome 5D/5T. The recombination event could occur in any generation from
F2
. Conclusions. Microsatellite loci could be used for identifi cation of introgressions from Aegilops mutica in wheat genome
(although with some limitations). Identifi ed plants with recombinant 5D/5Т chromosomes are perspective for future work.
Keywords: introgressive hybridization, Amblyopyrum muticum, microsatellite analysis, SSR loci, alien-substitution lines,
recombinant lines.
|
| issn |
2219-3782 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/177367 |
| citation_txt |
Introgressive wheat lines Triticum Aestivum/Amblyopyrum muticum as to structure of homoeologous group 5 chromosomes / T.S. Iefimenko, G. Fedak, M.Z. Antonyuk, T.K. Ternovska // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 16. — С. 83-86. — Бібліогр.: 14 назв. — англ. |
| work_keys_str_mv |
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| first_indexed |
2025-12-07T21:18:36Z |
| last_indexed |
2025-12-07T21:18:36Z |
| _version_ |
1850885870070005760 |