Сравнительный анализ аллельного состава микросателлитных локусов Brassica oleracea var. capitata L.

• Опубліковано в: Фактори експериментальної еволюції організмів
• Дата: 2015
• ISSN: 2219-3782
• Автори: Шаптуренко, М.Н., Печковская, Т.В., Вакула, С.И., Хотылева, Л.В.
• Формат: Стаття
• Мова: Російська
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2015
• Теми: Прикладна генетика і селекція
• Онлайн доступ: https://nasplib.isofts.kiev.ua/handle/123456789/177381
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Сравнительный анализ аллельного состава микросателлитных локусов Brassica oleracea var. capitata L. / М.Н. Шаптуренко, Т.В. Печковская, С.И. Вакула, Л.В. Хотылева // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 16. — С. 170-173. — Бібліогр.: 8 назв. — рос.

Aims. White cabbage is characterized by high level of intraspecifi c heterogeneity due to some biological features that
 causes diffi culties for breeding at creation constant form and maintaining of their genetic typicality. Thus, we studied
 the effectiveness the set of SSR markers for both white cabbage diversity analysis and pure lines genotyping. Methods.
 Molecular-genetic studies were performed trough SSR PCR. Effectiveness the set of markers was evaluated by counting
 allelic richness, PICs, heterogeneity, allele frequencies using GenAlEx 6.5. To determine B.o.var capitata diversity with
 low and high variations, Jaccard dissimilarity index was estimated by Darwin software. Results. 15 microsatellite loci
 were characterized for the information content, allelic frequencies and heterogeneity level. The effective multiallelic
 markers (Bo20TR, BoDCTD4, BoPC34, BoPLD1, BoCalc) with high information content (PIC > 0.7) that could be
 successfully used for the analysis of inter- and intravarietal polymorphism B. oleracea var.capitata were defi ned. Based
 on the SSR-evaluation and subsequent clustering the genetic structure of breeding collection was established, which
 showed that most experimental forms have a common ancestral genetic basis, in spite of different origins. Conclusions.
 Proposed set of informative SSR markers is a useful tool for the selection of genetically constant material, as well as
 divergent cross combinations for the hybrid breeding. Identifi ed donors of rare SSR alleles potentially could be a source
 of valuable genetic segregation for further B. oleracea improvement.
 Keywords: Brassica oleracea var. capitata L. f. alba DC, genetic diversity, SSR.