Сигнальна функція β-катеніну при адаптації дорослого міокарда ссавців до фізичних навантажень

• Date: 2015
• Main Authors: Пальчевська, О.Л., Балацький, В.В., Мацевич, Л.Л., Півень, О.О., Лукаш, Л.Л.
• Format: Article
• Language: Ukrainian
• Published: Інститут молекулярної біології і генетики НАН України 2015
• Series: Фактори експериментальної еволюції організмів
• Subjects: Генетика людини та медична генетика
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/177400
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Сигнальна функція β-катеніну при адаптації дорослого міокарда ссавців до фізичних навантажень / О.Л. Пальчевська, В.В. Балацький, Л.Л. Мацевич, О.О. Півень, Л.Л. Лукаш // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 16. — С. 225-229. — Бібліогр.: 19 назв. — укр.

Aims. Our current work is devoted to the adaptation of the myocardium of wild-type and animals with embryonic cardiospecifi c haploinsuffi ciency of β-catenin to physical training, namely smimming. Methods. Trangenic and wild-type animals were subjected to swimming test. The qPCR analysis of hypertrohic (ANP, BNP, β-MHC and α-MHC) and Wnt/ β-catenin pathway target genes (TCF4 and c-myc) was performed for control and trained mice. The HW/BW index was calculated to estimate myocardium hypertrophy development. Results. The increase of HW/BW index for trained animals was shown. The increase of hypertrophic genes (ANP, BNP, β-MHC, and α-MHC) were detected in both trained and control animals with β-catenin gene haploinsuffi ciency. It is also important to note that Wnt target genes were upregulated only in the β-catenin haploinsuffi cient stressed animals. Conclusions. The results show that β-catenin gene defi ciency leads to fetal (hypertrophic) genes violation even for unstressed animals. The activation of Wnt-signaling pathway was detected during adult myocardium adaptation to physical stress. These observations point to the conclusion that β-catenin gene function plays important role in the heart adaptation and remodeling during stress. Keywords: physiological cardiac hypertrophy, β-catenin haploinsuffi ciency, canonical Wnt signaling, heart adaptation, physical training.