Isolectins from Sambucus nigra fl owers and their effect on MGMT and P53 proteins amount in human cells in vitro
Aim. To characterise S.nigra fl ower isolectins in the aspect of their effect on MGMT and p53 proteins amount. Methods. Lectin preparations P1, P2 and P3 with defferent pI were obtained by isoelectric focusing. The Hep-2 cells were treated with S. nigra lectins: P1, P2, and a commercial SNA-I. Chang...
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| Datum: | 2015 |
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Інститут молекулярної біології і генетики НАН України
2015
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| Zitieren: | Isolectins from Sambucus nigra fl owers and their effect on MGMT and P53 proteins amount in human cells in vitro / L.L. Lylo, I.S. Karpova, K.V. Kotsarenko, L.L. Macewicz, T.A. Ruban, G.V. Dziuba, L.L. Lukash // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 16. — С. 273-276. — Бібліогр.: 22 назв. — англ. |
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Lylo, V.V. Karpova, I.S. Kotsarenko, K.V. Macewicz, L.L. Ruban, T.A. Dziuba, G.V. Lukash, L.L. 2021-02-15T15:53:58Z 2021-02-15T15:53:58Z 2015 Isolectins from Sambucus nigra fl owers and their effect on MGMT and P53 proteins amount in human cells in vitro / L.L. Lylo, I.S. Karpova, K.V. Kotsarenko, L.L. Macewicz, T.A. Ruban, G.V. Dziuba, L.L. Lukash // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 16. — С. 273-276. — Бібліогр.: 22 назв. — англ. 2219-3782 https://nasplib.isofts.kiev.ua/handle/123456789/177424 575.224 Aim. To characterise S.nigra fl ower isolectins in the aspect of their effect on MGMT and p53 proteins amount. Methods. Lectin preparations P1, P2 and P3 with defferent pI were obtained by isoelectric focusing. The Hep-2 cells were treated with S. nigra lectins: P1, P2, and a commercial SNA-I. Changes of protein amounts were detected by Western blot analysis. Results. Isolectins were characterized accoding to their carbohydrate specificity. They increased MGMT amount with different severity. p53 level under the P2 and SNA-I treatments reduced. These lectins were shown to effect the amount of MARP protein as well. Conclusions. All lectins studied enhanced the amount of MGMT and MARP proteins. Effect of lectins on p53 amount was the opposite. MGMT is supposed to be the primary target of lectin action which uses own lectin-dependent regulatory pathways of mammalian cells, and p53 level may be affected as a result of MGMT modulation. en Інститут молекулярної біології і генетики НАН України Фактори експериментальної еволюції організмів Генетика людини та медична генетика Isolectins from Sambucus nigra fl owers and their effect on MGMT and P53 proteins amount in human cells in vitro Article published earlier |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine |
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DSpace DC |
| title |
Isolectins from Sambucus nigra fl owers and their effect on MGMT and P53 proteins amount in human cells in vitro |
| spellingShingle |
Isolectins from Sambucus nigra fl owers and their effect on MGMT and P53 proteins amount in human cells in vitro Lylo, V.V. Karpova, I.S. Kotsarenko, K.V. Macewicz, L.L. Ruban, T.A. Dziuba, G.V. Lukash, L.L. Генетика людини та медична генетика |
| title_short |
Isolectins from Sambucus nigra fl owers and their effect on MGMT and P53 proteins amount in human cells in vitro |
| title_full |
Isolectins from Sambucus nigra fl owers and their effect on MGMT and P53 proteins amount in human cells in vitro |
| title_fullStr |
Isolectins from Sambucus nigra fl owers and their effect on MGMT and P53 proteins amount in human cells in vitro |
| title_full_unstemmed |
Isolectins from Sambucus nigra fl owers and their effect on MGMT and P53 proteins amount in human cells in vitro |
| title_sort |
isolectins from sambucus nigra fl owers and their effect on mgmt and p53 proteins amount in human cells in vitro |
| author |
Lylo, V.V. Karpova, I.S. Kotsarenko, K.V. Macewicz, L.L. Ruban, T.A. Dziuba, G.V. Lukash, L.L. |
| author_facet |
Lylo, V.V. Karpova, I.S. Kotsarenko, K.V. Macewicz, L.L. Ruban, T.A. Dziuba, G.V. Lukash, L.L. |
| topic |
Генетика людини та медична генетика |
| topic_facet |
Генетика людини та медична генетика |
| publishDate |
2015 |
| language |
English |
| container_title |
Фактори експериментальної еволюції організмів |
| publisher |
Інститут молекулярної біології і генетики НАН України |
| format |
Article |
| description |
Aim. To characterise S.nigra fl ower isolectins in the aspect of their effect on MGMT and p53 proteins amount. Methods. Lectin preparations P1, P2 and P3 with defferent pI were obtained by isoelectric focusing. The Hep-2 cells were treated with S. nigra lectins: P1, P2, and a commercial SNA-I. Changes of protein amounts were detected by Western blot analysis. Results. Isolectins were characterized accoding to their carbohydrate specificity. They increased MGMT amount with different severity. p53 level under the P2 and SNA-I treatments reduced. These lectins were shown to effect the amount of MARP protein as well. Conclusions. All lectins studied enhanced the amount of MGMT and MARP proteins. Effect of lectins on p53 amount was the opposite. MGMT is supposed to be the primary target of lectin action which uses own lectin-dependent regulatory pathways of mammalian cells, and p53 level may be affected as a result of MGMT modulation.
|
| issn |
2219-3782 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/177424 |
| citation_txt |
Isolectins from Sambucus nigra fl owers and their effect on MGMT and P53 proteins amount in human cells in vitro / L.L. Lylo, I.S. Karpova, K.V. Kotsarenko, L.L. Macewicz, T.A. Ruban, G.V. Dziuba, L.L. Lukash // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 16. — С. 273-276. — Бібліогр.: 22 назв. — англ. |
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2025-12-01T18:30:20Z |
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