Нанополімери на основі полі-ДМАЕМ – перспективні вектори для доставки генетичного матеріалу у рослинні клітини
Aims. Water soluble grafted polymers based on PDMAEMA (N,N-polydimethylaminoethyl metacrylate) are widely used in water systems for DNA and drug delivery to a target organs, tissues and cells. The significance of this research was in study of transformation efficiency and cytotoxic effects of nove...
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| Datum: | 2015 |
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| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | Ukrainian |
| Veröffentlicht: |
Інститут молекулярної біології і генетики НАН України
2015
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| Schriftenreihe: | Фактори експериментальної еволюції організмів |
| Schlagworte: | |
| Online Zugang: | https://nasplib.isofts.kiev.ua/handle/123456789/177492 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Нанополімери на основі полі-ДМАЕМ – перспективні вектори для доставки генетичного матеріалу у рослинні клітини / А.Ю. Бузіашвілі, Н.С. Фінюк, Р.С. Стойка, Я.Б. Блюм, А.І. Ємець // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2015. — Т. 17. — С. 117-120. — Бібліогр.: 10 назв. — укр. |
Institution
Digital Library of Periodicals of National Academy of Sciences of Ukraine| Zusammenfassung: | Aims. Water soluble grafted polymers based on PDMAEMA (N,N-polydimethylaminoethyl metacrylate) are widely used
in water systems for DNA and drug delivery to a target organs, tissues and cells. The significance of this research was in
study of transformation efficiency and cytotoxic effects of novel synthesized PDMAEMA based nanopolymers, namely:
BG-2, BG-2с, BG-2f, BG-2cq, BG-2cf, MP-1 and 83/6 on Nicotiana tabaccum L. protoplasts. Methods. In the process of
this study there was developed a new method of protoplast transformation with the use of PDMAEMA based nanopolymers.
Moreover, there were used standart plant cell culture and fluorescence light microsсopy methods. Results. The most effective
for protoplast transformation was nanopolymers of 83/6 type in concentration of 0.1 %. Such transformation conditions
showed the best efficiency and did not lead to significant effects on protoplast morphology. Conclusions. Finally, in a further
studies related to the use of PDMAEMA based nanopolymers in plant transformation there should be used nanopolymers of
83/6 type in concentration of 0.1 %.
Keywords: PDMAEMA, protoplasts, plasmid DNA, plant transformation. |
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